Alendronate and platelet-rich plasma in sheep anterior cruciate ligament

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J. Pharm. Pharmacogn. Res., vol. 12, no. 3, pp. 526-538, May-Jun 2024. DOI: https://doi.org/10.56499/jppres23.1845_12.3.526 Original Article Effect of alendronate and platelet-rich plasma in tendon-bones integration in sheep anterior cruciate ligament surgery [Efecto del alendronato y del plasma rico en plaquetas en la integración tendón-hueso en la cirugía del ligamento cruzado anterior ovino] Tangkas Sibarani1*, Bambang … Continue reading Alendronate and platelet-rich plasma in sheep anterior cruciate ligament

J. Pharm. Pharmacogn. Res., vol. 12, no. 3, pp. 526-538, May-Jun 2024.

DOI: https://doi.org/10.56499/jppres23.1845_12.3.526

Original Article

Effect of alendronate and platelet-rich plasma in tendon-bones integration in sheep anterior cruciate ligament surgery

[Efecto del alendronato y del plasma rico en plaquetas en la integración tendón-hueso en la cirugía del ligamento cruzado anterior ovino]

Tangkas Sibarani1*, Bambang Purwanto2, Ambar Mudigdo3, Brian Wasita3

1Doctoral Program, Faculty of Medicine, Universitas Sebelas Maret, Solo, Indonesia.

2Department of Internal Medicine, Dr. Moewardi Hospital/Faculty of Medicine UNS, Surakarta, Indonesia.

3Department of Anatomical Pathology, Dr. Moewardi Hospital/Faculty of Medicine UNS, Surakarta, Indonesia.

*E-mail: tangkas_sibarani@staff.uns.ac.id, sbrn.orto@gmail.com

Abstract

Context: Anterior cruciate ligament (ACL) reconstruction often faces challenges due to poor integration of the tendon with bone.

Aims: To evaluate the potential benefits of alendronate and platelet-rich plasma (PRP) in enhancing tendon-to-bone osteointegration in ACL surgery.

Methods: This was a post-test only control group experimental study with Ovis aries Linnaeus sheep as experimental animals. The sample was divided into four groups: the control group ACL reconstruction with calcaneal tendons given NaCl, the group given PRP, alendronate, and PRP and alendronate. Bone healing biomarkers (NF-kB, TNF-α, MMP-9, TGF-β1, and COL1A1) were examined through immunohistochemical analysis and histological studies to assess osteoblast counts and inflammatory tissue.

Results: There was a statistically significant (p<0.05) increase in MMP-9 and osteoblast count after alendronate and PRP administration. Administration of alendronate and PRP also increased other variables, namely TNF-α, COL1A1, and the level of inflammation, although not statistically significant (p>0.05). The intervention did not affect NF-kB and TGF-β1 (p> 0.05).

Conclusions: These results show that the administration of alendronate and PRP improves the healing of tendon-calcaneal ACL reconstruction surgery in sheep.

Keywords: alendronate; anterior cruciate ligament reconstruction; platelet-rich plasma.

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Resumen

Contexto: La reconstrucción del ligamento cruzado anterior (LCA) a menudo se enfrenta a desafíos debido a la mala integración del tendón con el hueso.

Objetivos: Evaluar los beneficios potenciales del alendronato y el plasma rico en plaquetas (PRP) en la mejora de la osteointegración tendón-hueso en la cirugía del LCA.

Métodos: Se trató de un estudio experimental de grupo control sólo postest con ovejas Ovis aries Linnaeus como animales de experimentación. La muestra se dividió en cuatro grupos: el grupo de control de reconstrucción del LCA con tendones calcáneos al que se administró NaCl, el grupo al que se administró PRP, alendronato, y PRP y alendronato. Se examinaron los biomarcadores de curación ósea (NF-kB, TNF-α, MMP-9, TGF-β1 y COL1A1) mediante análisis inmunohistoquímicos y estudios histológicos para evaluar el recuento de osteoblastos y el tejido inflamatorio.

Resultados: Hubo un aumento estadísticamente significativo (p<0,05) de la MMP-9 y del recuento de osteoblastos tras la administración de alendronato y PRP. La administración de alendronato y PRP también aumentó otras variables, concretamente el TNF-α, COL1A1 y el nivel de inflamación, aunque no de forma estadísticamente significativa (p>0,05). La intervención no afectó al NF-kB ni al TGF-β1 (p>0,05).

Conclusiones: Estos resultados demuestran que la administración de alendronato y PRP mejora la cicatrización de la cirugía de reconstrucción del LCA tendinoso-calcáneo en ovejas.

Palabras Clave: alendronato; reconstrucción del ligamento cruzado anterior; plasma rico en plaquetas.

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Citation Format: Sibarani T, Purwanto B, Mudigdo A, Wasita B (2024) Effect of alendronate and platelet-rich plasma in tendon-bones integration in sheep anterior cruciate ligament surgery. J Pharm Pharmacogn Res 12(3): 526–538. https://doi.org/10.56499/jppres23.1845_12.3.526
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