Antimicrobial, antioxidant, and antimutagenic activities of Gladiolus illyricus

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J Pharm Pharmacogn Res 2(4): 93-99, 2014. Original Article| Artículo Original Antimicrobial, antioxidant, and antimutagenic activities of Gladiolus illyricus. [Actividades antimicrobiana, antioxidante y antimutagénica de Gladiolus illyricus] Burcu Basgedika, Aysel Ugurb*, Nurdan Saraca aDepartment of Biology, Faculty of Sciences, Mugla Sitki Kocman University, Mugla, Turkey. bSection of Medical Microbiology, Department of Basic Sciences, Faculty of Dentistry, Gazi University, … Continue reading Antimicrobial, antioxidant, and antimutagenic activities of Gladiolus illyricus

J Pharm Pharmacogn Res 2(4): 93-99, 2014.

Original Article| Artículo Original

Antimicrobial, antioxidant, and antimutagenic activities of Gladiolus illyricus.

[Actividades antimicrobiana, antioxidante y antimutagénica de Gladiolus illyricus]

Burcu Basgedika, Aysel Ugurb*, Nurdan Saraca

aDepartment of Biology, Faculty of Sciences, Mugla Sitki Kocman University, Mugla, Turkey.
bSection of Medical Microbiology, Department of Basic Sciences, Faculty of Dentistry, Gazi University, Ankara, Turkey.
*E-mail:ayselugur@hotmail.com
Abstract

Context: In the present study, the ethanolic extracts of the aerial parts and the rhizomes of G. illyricus were obtained.
Aims: To determine the antimicrobial, antioxidant, and antimutagenic properties of G. illyricus extracts.
Methods: The antimicrobial activity was studied with the disc diffusion method and the antioxidant capacity by inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and the total antioxidant activity. The antimutagenic activity was investigated by Ames Salmonella/microsome mutagenic test. The bacterial mutant strains Salmonella typhimurium TA98 and TA100 were used to determine antigenotoxic potentials of the extracts.
Results: The ethanolic extracts of the aerial parts and the rhizomes showed moderate antimicrobial activity only on Bacillus subtilis. The IC50 value for DPPH radical of the aerial parts and rhizomes were 57.1 ± 1.3 and 48.1 ± 1.1 mg/mL, respectively. The total antioxidant activities of the aerial parts and the rhizomes of G. illyricus at 3.15 mg/mL concentration were 92.3 ± 1.4% and 91.5 ± 2.1%, respectively. These extracts showed antimutagenic effects at 0.5 and 5 mg/plate concentrations.
Conclusions: To our knowledge, this is the first study on antimicrobial, antioxidant, and antimutagenic activity of the ethanolic extracts of the aerial parts and the rhizomes from G. illyricus. Our results indicate that the used of these extracts would exert several beneficial effects by virtue of their antioxidant and antimutagenic activities. These activities could be an important topic in the medical and cosmetic fields.

Keywords: Ames test; antimicrobial; antioxidant; Gladiolus illyricus.

Resumen

Contexto: En el presente estudio se obtuvieron extractos etanólicos de las partes aéreas y los rizomas de G. illyricus.
Objetivos: Determinar las propiedades antimicrobiana, antioxidante y antimutagénica de los extractos de G. illyricus.
Métodos: La actividad antimicrobiana fue estudiada mediante el método de difusión en disco y la capacidad antioxidante por la inhibición del radical 2,2-difenil-1-picrilhidracilo (DPPH) y la actividad antioxidante total. La actividad antimutagénica fue investigada mediante la prueba mutagénica de Ames Salmonella/microsoma. Para determinar el potencial antigenotóxico de los extractos se utilizaron las cepas mutantes de Salmonella typhimurium TA98 y TA100.
Resultados: Los extractos etanólicos de las partes aéreas y rizomas mostraron actividad antimicrobiana moderada solamente frente a Bacillus subtilis. Los valores de IC50 para el radical DPPH de las partes aéreas y rizomas fueron 57,1 ± 1,3 y 48,1 ± 1,1 mg/mL, respectivamente. La actividad antioxidante total de las partes aéreas y rizomas de G. illyricus, a concentración de 3,15 mg/mL, fueron 92,3 ± 1,4% y 91,5 ± 2,1%, respectivamente. Los extractos mostraron efectos antimutagénicos a concentraciones de 0,5 y 5 mg/plate.
Conclusiones: No se encontró en la literatura algún estudio sobre la actividad antimicrobiana, antioxidante o anti-mutagénica de los extractos etanólicos de las partes aéreas y los rizomas de G. illyricus. Estos resultados indican que el uso de estos extractos podrían ejercer efectos beneficiosos en virtud de su actividad antioxidante y anti-mutagénica. Estas actividades podrían ser un tema importante en los campos médicos y cosméticos.

Palabras Clave: Antimicrobiano; antioxidante; ensayo de Ames; Gladiolus illyricus.

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Citation Format: Burcu Basgedik, Aysel Ugur, Nurdan Sarac (2014) Antimicrobial, antioxidant, and antimutagenic activities of Gladiolus illyricus. J Pharm Pharmacogn Res 2(4): 93-99.
This article has been cited by:
Kaur R, Nagpal AK, Katnoria JK  (2016) Antimutagenic potential of pollen grains of some medicinal plant species. International Journal of Pharmacy and Pharmaceutical Sciences 8(7): 232-236. Website

© 2014 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

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