Bioactivity of the endophytic fungus Fusarium sp.



J Pharm Pharmacogn Res 9(6): 755-765, 2021.

Original article

Antibacterial, antioxidant and neuroprotective activities of crude extract from the endophytic fungus Fusarium sp. isolate OQ-Fus-2-F from Euphorbia sp. plant

[Actividades antibacterianas, antioxidantes y neuroprotectoras del extracto crudo del hongo endofítico Fusarium sp. aislado OQ-Fus-2-F de Euphorbia sp. planta]

Omar S. Al-Qaralleh, Wael A. Al-Zereini*, Ahmed H. Al-Mustafa

Department of Biological Sciences, Faculty of Science, Mutah University, 61710 Al-Karak, Jordan.

*E-mail: wzereini@mutah.edu.jo

Abstract

Context: Human life is contested with the emergence of new infectious diseases and antibiotic-resistant pathogens, cellular deterioration due to oxidative stress, and age-related neurodegenerative disorders.

Aims: To evaluate the antibacterial, antioxidant, and acetylcholinesterase inhibitory activities of crude extract from the endophytic fungus Fusarium sp. isolate OQ-Fus-2-F as a basis for medical application.

Methods: Fusarium sp. isolate OQ-Fus-2-F was isolated from the stem of Euphorbia sp. plant. It was identified morphologically and based on 18S rRNA gene partial sequence. The isolated was cultivated in a malt extract glucose medium. Cell filtrate was extracted with ethyl acetate, and the crude extract was dried in vacuum. The crude extract was assessed for its antimicrobial activity through agar diffusion and micro-broth dilution assays; antioxidant activity was measured using DPPH and ABTS methods, and the anticholinergic activity was evaluated in terms of inhibition in acetylcholinesterase (AChE) activity.

Results: Crude extract of Fusarium sp. isolate OQ-Fus-2-F was more potent in inhibiting the growth of B. subtilis with a MIC value of 125 µg/mL. Its antioxidant activity was highly detected in ABTS than DPPH assay with IC50 values 37.5 ± 3.5 µg/mL and 191.3 ± 17.6 µg/mL, respectively. Moreover, the extract inhibited the AChE activity with IC50 177.0 ± 13.7 µg/mL.

Conclusions: Crude extract of Fusarium sp. isolate OQ-Fus-2-F has potential biomedical applications as it contains secondary metabolites with antimicrobial, antioxidant, and neuroprotective activates.

Keywords: acetylcholinesterase inhibition; Alzheimer’s disease; antibacterial activity; antioxidant activity; Fusarium sp.

This image has an empty alt attribute; its file name is jppres_pdf_free.png
Resumen

Contexto: La vida humana se enfrenta a la aparición de nuevas enfermedades infecciosas y patógenos resistentes a los antibióticos, el deterioro celular debido al estrés oxidativo y los trastornos neurodegenerativos relacionados con la edad.

Objetivos: Evaluar las actividades antibacterianas, antioxidantes e inhibidoras de la acetilcolinesterasa del extracto crudo del hongo endofítico Fusarium sp. aislado OQ-Fus-2-F como base para aplicaciones médicas.

Métodos: Fusarium sp. el aislado OQ-Fus-2-F se aisló del tallo de Euphorbia sp. planta. Se identificó morfológicamente y se basó en la secuencia parcial del gen ARNr 18S. El aislado se cultivó en medio de glucosa con extracto de malta. El filtrado celular se extrajo con acetato de etilo y el extracto crudo se secó al vacío. Se evaluó la actividad antimicrobiana del extracto crudo mediante ensayos de difusión en agar y dilución en microcaldo; La actividad antioxidante se midió utilizando métodos DPPH y ABTS y la actividad anticolinérgica se evaluó en términos de inhibición de la actividad acetilcolinesterasa (AChE).

Resultados: Extracto crudo de Fusarium sp. aislado OQ-Fus-2-F fue más potente para inhibir el crecimiento de B. subtilis con un valor de MIC de 125 µg/mL. Su actividad antioxidante fue altamente detectada en ABTS que en el ensayo DPPH con valores de CI50 de 37,5 ± 3,5 µg/mL y 191,3 ± 17,6 µg/mL, respectivamente. Además, el extracto inhibió la actividad de AChE con IC50 177,0 ± 13,7 µg/mL.

Conclusiones: Extracto crudo de Fusarium sp. aislado OQ-Fus-2-F tiene aplicaciones biomédicas potenciales ya que contiene metabolitos secundarios con activos antimicrobianos, antioxidantes y neuroprotectores.

Palabras Clave: acetilcolinesterasa inhibición; actividad antibacterial; actividad antioxidante; enfermedad de Alzheimer; Fusarium sp.

This image has an empty alt attribute; its file name is jppres_pdf_free.png
Citation Format: Al-Zereini W, Al-Mustafa A, Al-Qaralleh O (2021) Antibacterial, antioxidant and neuroprotective activities of crude extract from the endophytic fungus Fusarium sp. isolate OQ-Fus-2-F from Euphorbia sp. plant. J Pharm Pharmacogn Res 9(6): 755–765.

© 2021 Journal of Pharmacy & Pharmacognosy Research (JPPRes)