Category Archives: diabetic nephropathy

Fagonia cretica and Citrus paradise effects in diabetic rabbits

J Pharm Pharmacogn Res 5(6): 365-380, 2017.

DOI: https://doi.org/10.56499/jppres17.230_5.6.365

Original Article

Antidiabetic and renoprotective effect of Fagonia cretica L. methanolic extract and Citrus paradise Macfad. juice in alloxan induced diabetic rabbits

[Efecto antidiabético y renoprotector del extracto metanólico de Fagonia cretica L. y el jugo de Citrus paradise Macfad. en conejos diabéticos inducidos por aloxano]

Sairah H. Kamran1*, Rana M. Shoaib1, Mobasher Ahmad2, Saiqa Ishtiaq1, Rukhsana Anwar1

1Punjab University College of Pharmacy, University of the Punjab, Allama Iqbal Campus, Lahore – 54000, Pakistan.
2Pharmacy College, Gulab Devi Educational complex, Lahore, Pakistan.

*E-mail: sairah.shahid@gmail.com

Abstract

Context: Fagonia cretica is a medicinal herb reported to have flavanoids of potential therapeutic value and Citrus paradisi is a fruit, whose juice is of great therapeutic value due to its anti-hyperglycemic effects.

Aims: To determine anti-hyperglycemic and renal protective effect of methanolic extract of Fagonia cretica and Citrus paradisi juice (grapefruit juice) in alloxan induced diabetic rabbits.

Methods: Diabetes was induced in rabbits by alloxan monohydrate (150 mg/kg, i.p.). The therapies including Fagonia cretica methanolic extract (500 mg/kg), Citrus paradisi juice (7 mL/kg) and sitagliptin (10 mg/kg) were administered (p.o.) to diabetic groups for 14 days. The biochemical parameters, glucose, creatinine, urea, bilirubin, albumin, total protein, globulins and albumin/globulin ratio were estimated.

Results: Fagonia cretica extract and grapefruit juice therapy significantly (p<0.05) reduced glucose levels in diabetic rats. Fagonia cretica extract was more effective anti-hyperglycemic agent than Citrus paradisi juice and sitagliptin. Significant (p<0.05) improvement in kidney function was observed in treated groups, the plant extract showing significant improvement as compared to the other two treatments. The histopathological results verified improvement in structural damage of kidney, liver and pancreas with these treatments.

Conclusions: Fagonica cretica and Citrus paradisi juice therapy markedly improved hyperglycemia and kidney functions in alloxan-induced diabetic rabbits.

Keywords: alloxan; Citrus paradise; diabetes; Fagonia cretica; kidney.

Resumen

Contexto: Fagonia cretica es una hierba medicinal que posee flavonoides de potencial valor terapéutico y Citrus paradisi es una fruta cuyo jugo es de gran valor terapéutico debido a sus efectos antihiperglicémicos.

Objetivos: Determinar el efecto antihiperglicémico y protector renal del extracto metanólico de Fagonia cretica y el jugo de Citrus paradisi (jugo de toronja) en conejos diabéticos inducidos por aloxano.

Métodos: La diabetes fue inducida en conejos por monohidrato de aloxano (150 mg/kg, i.p.). Se administraron (p.o.) los tratamientos incluyendo el extracto metanólico de Fagonia cretica (500 mg/kg), jugo de Citrus paradisi (7 mL/kg) y sitagliptina (10 mg/kg) a grupos diabéticos durante 14 días. Se estimaron los parámetros bioquímicos, glucosa, creatinina, urea, bilirrubina, albúmina, proteína total, globulinas y relación albúmina/globulina.

Resultados: El extracto de Fagonia cretica y la terapia con jugo de toronja (p<0,05) redujeron significativamente los niveles de glucosa en ratas diabéticas. El extracto de Fagonia cretica fue un agente anti-hiperglucémico más eficaz que el jugo de Citrus paradisi y sitagliptina. Se observó una mejoría significativa (p<0,05) en la función renal en los grupos tratados, el extracto de la planta mostró una mejoría significativa en comparación con los otros dos tratamientos. Los resultados histopatológicos verificaron mejoría en el daño estructural del riñón, hígado y páncreas con estos tratamientos.

Conclusiones: La terapia con extracto de Fagónica cretica y jugo de Citrus paradisi mejoró notablemente la hiperglucemia y las funciones renales en conejos diabéticos inducidos por aloxano.

Palabras Clave: aloxano; Citrus paradise; diabetes; Fagonia cretica; riñón.

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Citation Format: Kamran SH, Shoaib RM, Ahmad M, Ishtiaq S, Anwar R (2017) Antidiabetic and renoprotective effect of Fagonia cretica L. methanolic extract and Citrus paradise Macfad. juice in alloxan induced diabetic rabbits. J Pharm Pharmacogn Res 5(6): 365–380. DOI: https://doi.org/10.56499/jppres17.230_5.6.365
This article has been cited by:
Dimple, Kumar A, Kumar V, Tomer V (2018) Traditional medicinal systems for treatment of diabetes mellitus: a review. International Journal of Pharmacy and Pharmaceutical Sciences 10(5): 7-17.

© 2017 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

Nephroprotective activity of Pterocarpus marsupium

J Pharm Pharmacogn Res 4(5): 174-186, 2016.

DOI: https://doi.org/10.56499/jppres16.136_4.5.174

Original Article

Nephroprotective role of alcoholic extract of Pterocarpus marsupium heartwood against experimentally induced diabetic nephropathy

[Papel nefroprotector del extracto alcohólico del duramen de Pterocarpus marsupium contra la nefropatía diabética inducida experimentalmente]

Pankaj Gupta1*, Preeti Sharma2, Kumari Shanno3, Vivek Jain3, Ashutosh Pareek3, Priyanka Agarwal4, Randhir Singh5, Veena Sharma2*

1Department of Pharmacology, DDPR Central Research Institute for Homoeopathy, Noida, India.
2Department of Biosciences & Biotechnology, Banasthali Vidyapeeth University, Banasthali, India.
3Department of Pharmacy, Banasthali Vidyapeeth University, Banasthali, India.
4Department of Plant Molecular Biology, University of Delhi, South Campus, New Delhi, India.
5College of Pharmacy, Maharishi Markandeshwar University, Mullana, Ambala, Harayana, India.

*E-mail: pankajgupta77@gmail.com (PG); drvshs@gmail.com (VS)

Abstract

Context: Heartwood of Pterocarpus marsupium has been widely reported for its effect on diabetes clinical or preclinically. However, role in diabetic complications is yet to be revealed.

Aims: To investigate the effect of alcoholic extract of the heartwood of Pterocarpus marsupiumin experimentally induced diabetic nephropathy in rats.

Methods: The streptozotocin (STZ) 55 mg/kg, i.p., once daily induced diabetes in Sprague-Dawley rats. These animals were treated orally with alcoholic extract of P. marsupium (100, 200 and 400 mg/kg) or glimepiride (10 mg/kg) for 60 days. Body weight, blood glucose, glycosylated hemoglobin (HBA1c), biochemical markers of renal function were estimated on day 30 and at the end of study (day 60). Kidney weight measurement, oxidative stress markers such as lipid peroxidation (TBARS), catalase, superoxide dismutase, reduced glutathione were estimated in kidney tissues, and histopathological evaluation was carried out at the end of study period.

Results: The administration of an alcoholic extract of P. marsupium showed a decrease in blood glucose, HBA1c, kidney weight, serum creatinine, blood urea nitrogen, serum uric acid, urea, urine volume, urine albumin and the level of TBARS. While the increase in urine creatinine, the activity of superoxide dismutase and glutathione were observed when compared to the diabetic control group. This effect was observed significantly at the highest dose of the plant extract. The histopathological study also confirmed that alcoholic extract prevented structural kidney damage.

Conclusions: These results suggest that the alcoholic extract of P. marsupium has renoprotective effects against STZ induced diabetic nephropathy.

Keywords: Antioxidant; biochemical markers; diabetic nephropathy; oxidative stress markers; Pterocarpus marsupium; renoprotective.

Resumen

Contexto: El duramen de Pterocarpus marsupium ha sido reportado ampliamente por su efecto sobre la diabetes, tanto clínica o preclínicamente. Sin embargo, el papel de las complicaciones diabéticas aún no se ha revelado.

Objetivos: Investigar el efecto del extracto alcohólico del duramen de Pterocarpus marsupiumin sobre la nefropatía diabética inducida experimentalmente en ratas.

Métodos: La estreptozotocina (STZ) 55 mg/kg, i.p., indujo diabetes en ratas Sprague-Dawley. Los animales se trataron oralmente con extracto alcohólico de P. marsupium (100, 200 y 400 mg/kg) o glimepirida (10 mg/kg) durante 60 días. El peso corporal, la glucosa en sangre, la hemoglobina glicosilada (HbA1c), los marcadores bioquímicos de la función renal se determinaron en el día 30 y al final del estudio (día 60). El peso del riñón, los marcadores de estrés oxidativo, tales como peroxidación lipídica (TBARS), catalasa, superóxido dismutasa y glutatión reducido se estimaron en los tejidos renales. La evaluación histopatológica se llevó a cabo al final del período de estudio.

Resultados: El extracto alcohólico de P. marsupium mostró una disminución en la glucosa en sangre, la HbA1c, el peso del riñón, creatinina sérica, nitrógeno ureico en sangre, ácido úrico en suero, urea, el volumen de orina, la albúmina de la orina y la concentración de TBARS. Se observó el aumento de la creatinina en orina, la actividad de la superóxido dismutasa y el glutatión en comparación con el grupo de control diabético. Este efecto se observó significativamente en la dosis más alta del extracto vegetal. El estudio histopatológico confirmó también que el extracto alcohólico impidió el daño renal estructural.

Conclusiones: El extracto alcohólico de P. marsupium tiene efectos nefroprotectores contra la nefropatía diabética inducida por STZ.

Palabras Clave: Antioxidante; marcadores bioquímicos; marcadores de estrés oxidativo; nefropatía diabética; Pterocarpus marsupium; renoprotectivo.

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Citation Format: Gupta P, Sharma P, Shanno K, Jain V, Pareek A, Agarwal P, Singh R, Sharma V (2016) Nephroprotective role of alcoholic extract of Pterocarpus marsupium heartwood against experimentally induced diabetic nephropathy. J Pharm Pharmacogn Res 4(5): 174-186. DOI: https://doi.org/10.56499/jppres16.136_4.5.174

© 2016 Journal of Pharmacy & Pharmacognosy Research (JPPRes)