Category Archives: FAPRONATURA

L-03: NATURAL PRODUCTS: A KEY-POINT FOR THE CUBAN PROGRAM OF TRADITIONAL AND NATURAL MEDICINE

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S3, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Lecture

L-03: NATURAL PRODUCTS: A KEY-POINT FOR THE CUBAN PROGRAM OF TRADITIONAL AND NATURAL MEDICINE

Perdomo-Delgado J.

Ministry of Public Health, Havana, Cuba. E-mail: tradicional@msp.sld.cu

 

In 1991 Commander in Chief Fidel Castro indicated to create the Program of Medicinal Plants in order to promote its rational and scientific usage, as a part of the development foreseen for the Cuban Pharmaceutical Industry. During the first half of the nineties’ medicinal plants were combined with other Traditional Medical Systems as a way to assure medical coverage in a time marked by the most difficult economic crisis suffered by the country after the Revolution. Undoubtedly, this was the source of the Program of Traditional and Natural Medicine (TNM) developed by the Ministry of Public Health since 1997. For the past twenty-four years Natural Products (NP) have been a key-point for this Program and after the Sixth Congress of the Cuban Communist Party, a Political Guideline was established to “pay maximum attention to the development of TNM”. Within this new momentum NP emerge again as an important topic. Considerations about NP are summarized in this lecture, in order to up-date the projections marked by the Ministry of Public Health and the rest of the members of the National Commission for TNM.

 

Citation Format: Perdomo-Delgado J (2015) Natural products: a key-point for the Cuban program of traditional and natural medicine. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S3. Abstract nr L-03.

L-04: NATURAL AND TRADITIONAL MEDICINE: MAGICAL REALISM?

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S4, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Lecture

L-04: NATURAL AND TRADITIONAL MEDICINE: MAGICAL REALISM?

Pérez-Peña J

Facultad Ciencias Médicas “Dr. Salvador Allende”, UCM de La Habana. E-mail: jppcdf@infomed.sld.cu

 

Magical realism as art of the twentieth century and its comparison with the Natural and Traditional Medicine is conceptualized. From the definitions of Natural Medicine, World Health Organization and other international organizations, the presence of the practice and development of the medicine in the world is identified. Mainstream criticisms that were made from the Western allopathic medicine are established. Finally, it refers to the need for an approach to this phenomenon from the public health and some propositions are made.

 

Citation Format: Pérez-Peña J (2015) Natural and traditional medicine: magical realism? [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S4. Abstract nr L-04.

PNA-28: TROPICAL FRUITS POLYPHENOLS: EXCELLENT INHIBITORS OF THE OXIDATION OF THE LDL

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S120, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNA-28: TROPICAL FRUITS POLYPHENOLS: EXCELLENT INHIBITORS OF THE OXIDATION OF THE LDL

Herrera E1, Fernández R1, Bebelagua Y2, García MA1, Rodríguez JL3.

1Pharmacy and Food Institute, University of Havana, St. 222, No. 2317, Havana ZC 13600, Cuba. E-mail: eherrera@ifal.uh.cu
2Center of Molecular Immunology. St 216, corner 15, Atabey, Playa, Havana ZC 11 600, Cuba.
3Food Industry Research Institute. Carretera al Guatao km 3 ½, Havana ZC 19200, Cuba.

 

Introduction: Epidemiological studies have demonstrated that fruit polyphenols can inhibit the oxidation of lipoproteins of low density (LDL), factor that trigger cardiovascular illnesses. Therefore, this work evaluated the antioxidant power of tropical fruits polyphenols, that never have been studied, present in papaya (Carica papaya), soursop (Annona muricata), mammee (Pouteria sapota), guava (Psidium guajava), banana cv. vietnamese (Musa paradisiaca) tamarind (Tamarindus indicus). Methods: The oxidation was performed at 37°C with different concentrations of polyphenols. The oxidation products were measured by the 2-tiobarbituric acid method. The IC50 was estimated as an antioxidant quality index and the Antioxidant Potential per Serving (APS) as a parameter that categorize the fruits relating the quality and quantity of phenols. Results: The Tamarindus indicus and Pouteria sapota evidenced the best antioxidant quality of polyphenols. Likewise, the Tamarindus indicus and Psidium guajava showed the biggest APS.

 

Citation Format: Herrera E, Fernández R, Bebelagua Y, García MA, Rodríguez JL (2015) Tropical fruits polyphenols: excellent inhibitors of the oxidation of the LDL. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S120. Abstract nr PNA-28.

PNA-27: ANTIOXIDANT CAPACITY AND FATTY ACID PROFILE OF Swinglea glutinosa (BLANCO) MERR, CULTIVATED IN CUBA

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S119, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNA-27: ANTIOXIDANT CAPACITY AND FATTY ACID PROFILE OF Swinglea glutinosa (BLANCO) MERR, CULTIVATED IN CUBA

Goya E1, Jorge E1, Saucedo Y1, Vander Heyden Y2, Le Thi Cam Tú1.

1Department of Pharmacy, Faculty of Chemical-Pharmacy, Central University of Las Villas, Santa Clara, 54830, Villa Clara, Cuba. E-mail: egoya@uclv.edu.cu, egoya@dcemail.com
2Department of Analytical Chemistry and Pharmaceutical Technology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel-VUB, Belgium.

 

Introduction: The small tropical tree Swinglea glutinosa (Blanco) Merr. is a member of the Rutaceae family. Originally brought to South America from Southeast Asia, it is used as an ornamental plant in Cuba and as a natural barrier in rural areas and gardens. Extracts from this tree have been assessed for cytotoxic and antimalarial activity in previous studies but never been evaluated its antioxidant activity. Material and Methods: The antioxidant capacity of the methanolic extracts and the fatty acid composition of leaves and fruits S. glutinosa (Blanco) Merr was investigated. Six different chemical methods were used to determine the antioxidant capacity. The fatty acid composition was analyzed using gas chromatography. Results: The IC50 value of the extracts was determined being 28.2 g/mL to leaf extract and 10 μg/mL to fruit extract (in the DPPH method. The concentration of the extracts resulted in increased in the ferric reducing antioxidant power to both extracts tested. The amount of total phenolic content was detected as 48.5 mg and 35.9 gallic acid equivalent (GAE)/g in the fruit and the leaf extract, respectively; meanwhile the total antioxidant capacity was 94.93 and 75.3 mg ascorbic acid equivalent (AE)/g for fruits and leaves, respectively. The peroxidation lipid assays (FTC and TBA methods) shows highest antioxidant effect for the leaf extract. Conclusions: The results permit to deduce that the fruit extract has highest anti-radical effect and the leaf extract has highest effect against the lipid peroxidation. The major fatty acid in the composition of Swinglea glutinosa was found to be the ω6 (linoleic) and ω9 (oleic) acids by GC analysis, with 0.5 % for both. This study reveals that Swinglea glutinosa is an attractive source of ω fatty acid components, especially the essential ones, as well as of effective natural antioxidants.

 

Citation Format: Goya E, Jorge E, Saucedo Y, Vander Heyden Y, Le Thi Cam Tú (2015) Antioxidant capacity and fatty acid profile of Swinglea glutinosa (Blanco) Merr, cultivated in Cuba. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S119. Abstract nr PNA-27.

PNA-26: In vitro AND in vivo ANTIOXIDANT EFFECT OF THE ETHANOLIC EXTRACT OF Solanum diploconos FLOWERS

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S118, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNA-26: In vitro AND in vivo ANTIOXIDANT EFFECT OF THE ETHANOLIC EXTRACT OF Solanum diploconos FLOWERS

Venturi I1, Delgado-Roche L2, Menéndez R2, Morales RA2, Valdés O3, Hernández Y3, Malheiros A1, de Souza MM1, Cechinel-Filho V1, Rodeiro I2.

1Programa de Pós-graduação em Ciências Farmacêuticas, Universidade do Vale do Itajaí, Santa Catarina, Brazil. E-mail: ivonilce.venturi@gmail.com
2Department of Pharmacology, 3Department of Chemistry Center of Marine Bioproducts, Havana, Cuba.

 

Introduction: Solanum diploconos is a species that belongs to the family of Solanaceae and the gender Solanum. The plant grows in Brazil, mainly in the regions of Santa Catarina. The scientific literature contains wide information on its botanic characteristics; however, little is known on the chemical composition and the biological properties of the plant. Thus, the aim of the present work was to evaluate the in vitro and in vivo antioxidant effect of the ethanolic extract of Solanum diploconos flowers. Material and Methods: The ethanolic extract was obtained by maceration and concentrated in a rotary evaporator at 40°C. The total content of flavonoids and phenolic compounds in the flowers extract was determined using standard procedures. The in vitro characterization of flowers included the ferric reducing power (FRAP), the inhibitory effect of brain phospholipid peroxidation, as well as the DPPH and superoxide anion scavenging capacity. Furthermore, a mouse model of benzo(a)pyrene (BaP)-induced oxidative stress was used to assess the in vivo antioxidant capacity of Solanum diploconos flowers. Results: The ethanolic flowers extract contains flavonoids (19.02 µg/g of dried extract) and phenolic compounds (3.14 mg⁄g of dried extract). The S. diploconos flowers inhibited (85.23%) the brain phospholipid oxidation (IC50=68.86 µg/mL), meanwhile the DPPH and superoxide anion scavenging capacity (IC50= 97.74 and 110.90 µg/mL, respectively) increased as the concentra-tion increases. The ferric reducing power was comparable with ascorbic acid. In addition, the oral administration of flower extract (10, 100 and 200 mg/kg) during a week prevented BaP-induced glutathione levels diminishment, together with a significant reduction (p<0.05) of lipid and protein damages. Conclusions: The present results demonstrated by the first time the in vitro and in vivo antioxidant capacity of Solanum diploconos flowers. Thus, the present work supports the potential use of this plant as a natural source of antioxidants to prevent oxidative stress-associated diseases.

Citation Format: Venturi I, Delgado-Roche L, Menéndez R, Morales RA, Valdés O, Hernández Y, Malheiros A, de Souza MM, Cechinel-Filho V, Rodeiro I (2015) In vitro and in vivo antioxidant effect of the ethanolic extract of Solanum diploconos flowers. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S118. Abstract nr PNA-26.

PNA-25: ANTIOXIDANT AND ANTI-INFLAMMATORY EFFECTS OF PROTEOLYTIC FRACTION FROM Vasconcellea cundinamarcensis

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S117, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNA-25: ANTIOXIDANT AND ANTI-INFLAMMATORY EFFECTS OF PROTEOLYTIC FRACTION FROM Vasconcellea cundinamarcensis LATEX ON HAIRLESS MICE SKIN IRRADIATED WITH MULTIPLE DOSES OF UVB

Freitas KM1, Salas CE2, Lopes MTP1.

Departments of 1Pharmacology and 2Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. E-mail: cesbufmg@yahoo.com

 

Introduction: UV radiation induces skin damage by imbalance of the endogenous antioxidant system leading to an increase of free radical and inflammation. The P1G10 fraction from Vasconcellea cundinamarcensis latex, rich in cysteine protease, displays angiogenic, mitogenic and anti-inflammatory activities and enhances wound healing on different cutaneous lesion models. Here, we investigated the possible antioxidant and anti-inflammatory effects of topical P1G10 formulation against damage induced by multiples doses of UVB on mice skin. Methods: Hairless mice (n=49) were irradiated with UVB light (240 mJ/cm2/312 nm) on the dorsal area (protocol CETEA 174/2010). P1G10 (0.1-1.0% or vehicle – Natrosol®) was applied topically after daily UVB exposure for 10 days. One day after last treatment, the animals were anaesthetized, fragments of damaged skin were excised and analyzed for different markers. The catalase and GSH (Glutathione) activities were measured using spectrophotometric and fluorescent assays, respectively. Cytokines levels were determined by ELISA. The data were expressed as mean ± standard error and statistically analyzed by one way ANOVA, Newman-Keuls post-test. Results: The catalase activity was significantly enhanced by 0.1% or 0.5% P1G10 treatment (0.04 ± 0.003 vs 0.02 ± 0.003 ΔABS/µg protein/min – vehicle, p<0.05). Furthermore, a decrease in GSH, TNF-α and IL1β (p<0.05) levels was observed following each dose of P1G10. In addition, VEGF levels were reduced at 0.1% of P1G10 (2.70 ± 0.22 vs 3.06 ± 0.28 ρg/mg – vehicle, p<0.05). Conclusions:  The changes in catalase/GSH activities and cytokines levels suggest a role for P1G10 as antioxidant and anti-inflammatory, respectively, on tissue repair following UVB-induced damage. Financial Support: CNPq, FAPEMIG and CAPES.

 

Citation Format: Freitas KM, Salas CE, Lopes MTP (2015) Antioxidant and anti-inflammatory effects of proteolytic fraction from Vasconcellea cundinamarcensis latex on hairless mice skin irradiated with multiple doses of UVB. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S117. Abstract nr PNA-25.

PNA-29: EVALUATION OF ANTIOXIDANT ACTIVITY AND ACUTE TOXICITY OF AQUEOUS FRACTION OF Dichrostachys cinerea LEAVES

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S121, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNA-29: EVALUATION OF ANTIOXIDANT ACTIVITY AND ACUTE TOXICITY OF AQUEOUS FRACTION OF Dichrostachys cinerea (L.) WIGHT & ARN. (Mimosaceae) LEAVES

Ribalta V1, Torres L2, Hernández E1, Sueiro M1, Armas Y1, Ruz V1.

1Pharmacy Department, Chemistry and Pharmacy Faculty. Central University of Las Villas, Santa Clara, Cuba. Road to Camajuaní km 5 ½. e-mail: venancior@uclv.edu.cu. Phone:42- 222544.
2Pharmacy Department, Food and Pharmacy Institute. University of Havana. Havana, Cuba.

 

Introduction: The aim of this study was to evaluate the in vitro antioxidant properties and the acute oral toxicity of the aqueous fraction of the D. cinerea leaves (AFDC) in Sprague-Dawley rats. Material and Methods: Plant material: Leaves were collected in the Botanical Garden of the university. Plant sample was identified as Dichrostachys cinerea by a taxonomic expert of the above institution. Phytochemical procedure: Dry powder (30 g) was extracted with methanol using Soxhlet apparatus. Methanol was removed under reduced pressure at 40°C to obtain a residue. It was suspended in water and partitioned with n-hexane to degrease. AFDC was collected and store at 2-8 °C until use. Antioxidant properties of D. cinerea: The ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl dihydrazyl (DPPH) and Fe2+-chelating assays were used. The total phenolic (TPC) and total flavonoids contents (TFC) were estimated by Folin-Ciocalteu and AlCl3 methods respectively, using galic acid (GA) and quercetin (Q) as standards. Acute Oral Toxicity: Test was carried out according to the single doses method on Sprague-Dawley rats at 2000 mg/kg body weight. Results and Discussion: Positive results were found for flavonoids, tannins and saponins. Antioxidant activity: AFDC had effective antioxidant properties giving IC50 values of 5.83 ± 0.03 µg/mL   by ferric ions (Fe3+)- reducing power, 3.00 ± 0.00 µg/mL by DPPH assay and 8.28 ± 1.04 µg/mL by ferrous ions (Fe 2+) chelating activity. The total phenolic content (TPC) and total flavonoids content (TFC) were 243 ± 8.71 µg GAE/mg and 20 ± 0.48 µg QE/mg, respectively. Acute Toxicity: There was no mortality or behavioral abnormality in rats treated orally with a dose of 2000 mg/kg body weight. Conclusions: The results obtained suggest that the aqueous fraction of D. cinerea leaves possess significant in vitro antioxidant activity and can be considered as safe up to 2000 mg/kg body weight.

 

Citation Format: Ribalta V, Torres L, Hernández E, Sueiro M, Armas Y, Ruz V (2015) Evaluation of antioxidant activity and acute toxicity of aqueous fraction of Dichrostachys cinerea (L.) Wight & Arn. (Mimosaceae) leaves. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S121. Abstract nr PNA-29.

PNAI-35: ANTIMICROBIAL ACTIVITY OF THE TOTAL EXTRACT, FRACTIONS AND PURE COMPOUNDS OF THE LEAVES OF Excoecaria lucida SW.

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S127, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-35: ANTIMICROBIAL ACTIVITY OF THE TOTAL EXTRACT, FRACTIONS AND PURE COMPOUNDS OF THE LEAVES OF Excoecaria lucida SW.

Gross C1, Munyewu B2, Ochoa A2, Escalona JC2, Camacho M3, Fechine J4, Sobral M4.

1Instituto Superior de Ciencias Médicas, Facultad 1, Avenida de las Américas, Santiago de Cuba.
2Departamento de Farmacia, Facultad de Ciencias Naturales, Universidad de Oriente. Patricio Lumumba s/n, Santiago de Cuba, Cuba. E-mail: aochoa@cnt.uo.edu.cu
3Centro de Estudios de Biotecnología Industrial, Facultad de Ciencias Naturales, Universidad de Oriente. Patricio Lumumba s/n, Santiago de Cuba, Cuba.
4Laboratorio de Tecnología Farmacéutica, Post-graduación en Productos Naturales y Sintéticos Bioactivos, Instituto de Ciencias de la Salud, Universidad Federal de Paraíba, Brasil.

 

Introduction: The Cuban population utilizes Excoecaria lucida Sw. (Aité) (Euphorbiaceae) for medicinal purposes (antimicrobial, antiasthmatic, toothache, callus destruction); however, the available scientific information is insufficient. Considering this background a study of its phytochemical and pharmacologic potential to support its terapeutic use is required. Methods: Seven samples obtained from the leaves of this plant species were  tested for the antimicrobial  activity [Total extract (10 and 100 mg/mL); ethyl acetate and butanolic fraction (5 and 100 mg/mL, respectively); Ellagic acid (1 and 20 mg/mL); 3,3´,4´-Tri-O-methylellagic acid; 3,3´,4´-Tri-O-methylellagic-4-O-B-D-glucopyranoside and Corilagin (1 mg/mL)]. The diffusion in disc method (Kirby-Bauer) was used for the six bacteria (Staphylococcus aureus; Escherichia coli; Enterococcus faecalis; Pseudomonas aeruginosa; Bacillus cereus; Bacillus megaterium), four fungi (Aspergillus niger; Alternaria alternata; Fusarium solani; Aspergillus versicolor) and a yeast (Candida albicans). The Minimum Concentration Inhibitory (MCI) and Minimum Concentration Bactericide (MCB) were determined using the broth dilution method. Results: The positive results were for the Total extract (100 mg/mL, sample 1) and ethyl acetate fraction (100 mg/mL, sample 2) that presented activity against Staphylococcus aureus, Enterococcus faecalis, Bacillus cereus and Bacillus megaterium, also butanolic fraction (100 mg/mL) had activity against Bacillus cereus and Bacillus megaterium. The sample had no antifungal activity. Conclusions: The total extract and ethyl acetate fraction had a large spectrum of antibacterial action as compared to the butanolic fraction. Ethyl acetate fraction demonstrated more antibacterial activity against the bacteria tested. The MCI and MCB were determined for the samples 1 and 2, with this confirming the highest antibacterial activity of the ethyl acetate fraction.

Citation Format: Gross C, Munyewu B, Ochoa A, Escalona JC, Camacho M, Fechine J, Sobral M (2015) Antimicrobial activity of the total extract, fractions and pure compounds of the leaves of Excoecaria lucida Sw. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S127. Abstract nr PNAI-35.

PNAI-33: CYTOSTATIC AND CYTOTOXIC EVALUATION OF EXTRACTS FROM FIVE CUBAN PLANTS

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S125, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-33: CYTOSTATIC AND CYTOTOXIC EVALUATION OF EXTRACTS FROM FIVE CUBAN PLANTS WITH POSSIBLE ANTIMICROBIAL, ANTIPARASITIC AND ANTI-PROLIFERATIVE EFFECT

Mancebo B, Regalado AI, Sánchez LM.

National Center for Animal and Plant Health (CENSA), PO Box 10, ZC 32700, San José de las Lajas, Mayabeque, Cuba. E-mail: betty@censa.edu.cu, adi@censa.edu.cu, luzmaria@censa.edu.cu

 

Introduction: The inhibition of seed germination and Artemia salina lethality test are considered a useful tool for the preliminary toxicity determination of plant extracts. The objective of the present work was to determine the cytostatic and cytotoxic effect of total methanol extracts from leaves and stems obtained from the species Ta, Th, Gl, Va and Cc belonging to Bignonacea family; and hexane, ethyl acetate and methanol fractions. Material and Methods: The cytostatic effect was determined by the seed germination inhibition test of Solanum lycopersicum (tomato) and Lactuca sativa (lettuce) of total methanol extracts and fractions of the species under study. Cytotoxicity was performed by Artemia salina lethality test. Results: From 10 of the total methanol extracts studied, four showed cytostatic effect at the tested concentrations, where Gl (leaves) extract showed the best effect. Moreover, of the 15 fractions studied, Gl (leaves) acetate, Th (leaves) acetate and methanol and Cc (leaves) acetate, showed promising cytostatic effect, where Th (leaves) acetate fraction showed the highest activity. As regards toxicity study with brine shrimp larvae, all total methanol extracts showed moderate activity, however Gl (leaves), Ta (leaves) and Th (leaves) fractions were classified as highly toxic. Conlusions: The cytostatic and cytotoxic activities of the species studied are showed by the results of this research, constituting the first report of the same, which could have active substances associated with promising antimicrobial, antiproliferative and antiparasitic activity.

 

Citation Format: Mancebo B, Regalado AI, Sánchez LM (2015) Cytostatic and cytotoxic evaluation of extracts from five Cuban plants with possible antimicrobial, antiparasitic and anti-proliferative effect. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S125. Abstract nr PNAI-33.

PNAI-32: ANTIBACTERIAL EFFECT OF Costus spiralis LEAVES EXTRACT ON PATHOGENIC STRAINS OF Vibrio cholera

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S124, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-32: ANTIBACTERIAL EFFECT OF Costus spiralis LEAVES EXTRACT ON PATHOGENIC STRAINS OF Vibrio cholera

Pérez C, Falero A, Hung BR, Ledón T, Fando R.

Centro Nacional de Investigaciones Científicas, Ave 25 Esq 158, Playa. La Habana, Cuba. E-mail: talena.ledon@cnic.edu.cu

 

Introduction: The use of remedies from plant origin covers a wide variety of maladies and constitutes an alternative way to antibiotic therapy, which otherwise seems to be no longer promising due to antibiotics widespread resistance among pathogenic microorganisms. Active principles having antimicrobial activity may be extracted and purified from plants for developing new drugs. Among several illnesses that have historically scourged man, cholera has been potentially epidemic and one of the most outstanding. The bacterium Vibrio cholerae, the causal agent, can be eliminated with antibiotics so that besides the traditional treatment of rehydration via oral or intravenous, antibiotics such as tetracycline, ciprofloxacin, norfloxacin or azithromycin are commonly applied. Material and Methods: The antimicrobial effect of Costus spiralis (Roscoe) leaves extracts on various pathogenic strains of Vibrio cholerae was assayed in vitro by means of the agar plate diffusion technique. Fresh leaves from this plant were picked up, ovendried for 48 h at 50°C, powdered and finally ethanol-extracted. After drying, the remaining residue was suspended in distilled water up to 100 mg/mL (w/v) and the antimicrobial assays carried out. The pathogenic strains representing the pandemics of the 20th Century: C7258 (O1, El Tor, Ogawa), C6706 (O1, El Tor, Inaba), O395 (O1, Classic, Ogawa), CRC266 (O139) and 569B (O1, Classic, Inaba) were apparently killed, as judged by halos of inhibition of growth in the assays. Similar minimal inhibitory concentrations (MICs) of the extracts for the various strains were obtained. The above results were similar to those from ampicillin. Results and Conclusions: Costus spiralis may be used as a source of active principles against Vibrio cholera and may be considered as an alternative way to antibiotic therapy for cholera, mostly in those cases where the disease becomes epidemic and sanitary conditions were not promptly available.

 

Citation Format: Pérez C, Falero A, Hung BR, Ledón T, Fando R (2015) Antibacterial effect of Costus spiralis leaves extract on pathogenic strains of Vibrio cholera. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S124. Abstract nr PNAI-32.