Category Archives: FAPRONATURA

PNAI-31: ANTIMICROBIAL ACTIVITY AND PHENOLIC COMPOUND IN TWO CUBAN Phyllanthus SPECIES

J Pharm Pharmacogn Res 3(suppl. 1): S123, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-31: ANTIMICROBIAL ACTIVITY AND PHENOLIC COMPOUND IN TWO CUBAN Phyllanthus SPECIES

Chevalier P1, Galdós M2, Electra E1.

1Universidad de Ciencias Médicas “Carlos J. Finlay”, Camagüey, Cuba. E-mail: elenaelectrarabi@gmail.com
2Centro de Inmunología y Productos Biológicos (CENIPBI), Camagüey, Cuba.

 

Introduction: Various species of the genus Phyllanthus are used in natural medicine. Multiple studies in vitro and in vivo tested antiviral, anti-tumor, anti-inflammatory and anti-microbial properties. Numerous investigations show that the origin of the antiviral activity of these species is due to the presence of phenolic compounds that inhibit DNA polymerase and reverse transcriptase in the replication of DNA. Studies in two endemic species of Cuba demonstrate antiviral activity evidenced by: inactivation of surface antigen Hepatitis B viral, inhibition of multiplication of herpes simplex type 2 virus(HSV-2)  in human foreskin fibroblasts (FPH) and bovine type 1 herpes virus (BHV-1) in bovine kidney cells (MDBK). The possibility of antimicrobial activity of extracts with antiviral properties would provide added value for topical use, since many opportunistic microorganisms infect broken skin regions. It is for these reasons that the work aims at studying the antimicrobial action of aqueous extracts of Phyllanthus chamaechristoides ssp baracoensis URB. (Webster) and Phyllanthus orbicularis HBK against reference strains of pathogenic organisms such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans rigged to the quantification of phenolic compounds present in these. Material and Methods: For antimicrobial activity against the organisms, microdilution in broth technique was used. The quantification of the phenolic compounds was performed by colorimetric technique of colored complexes with ferric salt by reaction in alkaline medium with absorbance reading at 490 nm. Results: None of the extracts had a significant inhibition allowing antimicrobial activity correlate with the concentration of phenolic compounds. The possibility of microbial action of these plants is not discarded as fractions of low polarity are positive for other species.

Citation Format: Chevalier P, Galdós M, Electra E (2015) Antimicrobial activity and phenolic compound in two Cuban Phyllanthus species. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S123. Abstract nr PNAI-31.

PNAI-30: In vitro ANTILEISHMANIAL ACTIVITY OF Heliotropium indicum LINN AND Cordia dentata POIR FRACTIONS

J Pharm Pharmacogn Res 3(suppl. 1): S122, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-30: In vitro ANTILEISHMANIAL ACTIVITY OF Heliotropium indicum LINN AND Cordia dentata POIR FRACTIONS

Gómez-Estrada H1, Mercado-Camargo J1, Cervantes-Ceballos L1, Jiménez-Villalobos T1, Robledo-Restrepo S2.

1Grupo de Investigación Química de Medicamentos. Facultad Ciencias Farmacéuticas. Universidad de Cartagena. Cartagena-Colombia. E-mail: hgomeze@unicartagena.edu.co
2Programa de Estudio y Control de Enfermedades Tropicales PECET. Universidad de Antioquia, Medellín-Colombia.

 

Introduction: Medicinal plants play an important role between different biological processes as antitumor, cytotoxicity, antimicrobial, anti-inflammatory, anti-estrogen, anti-allergic, antioxidant, among other. Colombia has about 10% of all plant species in the world, being the North coast with highest medicinal herbs few studied. This study aims to evaluate in vitro cytotoxic activity on human promonocytic cell line U937 and leishmanicidal activity against intracellular amastigotes of Leishmania (V) panamensis (MHOM/CO/87/UA140-EpiR-GFP strain) of fractions obtained from leaves Heliotropium indicum Linn and Cordia dentata Poir. Material and Methods: The plants were collected in 2013 at the North of Bolivar (Colombia). The cytotoxic activity was performed using 2,5-diphenyl-tetrazolium enzymatic method 3-[4,5-dimethylthiazol-2-yl] (MTT) and antileishmanial assessment by flow cytometric technique. The assays were done by triplicate in at least two independent experiments, as positive control was used amphotericin B. Results were expressed as fifty cytotoxic concentrations (CC50), concentration necessary to kill 50% of cells, and fifty effective concentrations (EC50) calculated by Probit analysis (Parametric method of linear regression that permits doses-response analysis); it was similarly determined the Selectivity Index (SI). Results: H. indicum (Hi) fractions were more cytotoxic than C. dentata (Cd) against cell line U937. Hi-I- 18B and Hi-I-18E fractions were considered as potentially cytotoxic (CC50 11.7 and 13.9 mg/mL). Moreover, the Cd-I-13C fraction was midly cytotoxic (CC50 139.1 µg/mL). The fractions were first screened for antileishmanial activity against Leishmania (V) panamensis at 13. 5 ± 0.3 mg/mL, IS = 0.9 (Hi-I- 18B) and 11.5 ± 2.6 mg/mL, IS = 1.2 (Hi-I-18E) showed % growth inhibition were 18.5 and 21.8%. Cd-I-13C at 24.5 mg/mL, IS = 5.7, growth inhibition was 54.3%. Conclusions: This suggests that plants species are rich potential source of molecules useful for treatment of cancer and parasitic diseases. Financial Support: COLCIENCIAS, Colombia (Project 1107-569-33684/512-2012)

Citation Format: Gómez-Estrada H, Mercado-Camargo J, Cervantes-Ceballos L, Jiménez-Villalobos T, Robledo-Restrepo S (2015) In vitro antileishmanial activity of Heliotropium indicum Linn and Cordia dentata Poir fractions. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S122. Abstract nr PNAI-30.

PTS-40: DERMAL AND OCULAR IRRITATION STUDIES OF TWO CREAMS INTENDED FOR ITS USE FOR PSORIASIS

J Pharm Pharmacogn Res 3(suppl. 1): S132, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PTS-40: DERMAL AND OCULAR IRRITATION STUDIES OF TWO CREAMS INTENDED FOR ITS USE FOR PSORIASIS

Rivero Y1, González Y1, Mancebo A1, Scull I2, Arteaga ME1, González C1, Bada AM1.

1Centro Nacional para la Producción de Animales de Laboratorio (CENPALAB), La Habana, Cuba. E-mail: yesenia.rivero@cenpalab.inf.cu
2Instituto Tecnológico “Rubén Martínez Villena”, La Habana, Cuba.

 

Introduction: The introduction of novel materials into clinical practices requires safety evaluations as well as an understanding of the impact of the products on human health. Sebomori and Psomori are two creams intended for use as a lubricant for the skin affected by psoriasis. The active ingredients of Sebomori are extracts of plants Jacobinia mohintle and Morinda citrifolia, while the active ingredients of Psomori are extracts of plant Morinda citrifolia and seed oil of Azaridachta indica (Nim). Skin and eyes have the highest risk of exposure to Psomori and Sebomori, because deposition to the superficial organs has the potential to be a major route of exposure during the manufacturing, use, and disposal of these products. Material and Methods: This study was performed to examine the potential irritating of Psomori and Sebomori on the skin and eyes. The dermal and eye irritation study was performed using rabbits according to the Organisation for Economic Co-operation and Development (OECD) Guidelines 404 and 405, respectively. The substances in the test were the maximum allowable for administration according to regulations, using the commercial formulation. Results: No dermal responses, including erythema/eschar or edema, were found in rabbits treated with tested subtances. No rabbits exhibited corneal opacity, abnormality of the iris, or chemosis eye at any time point after the application of Sebomori and Psomori. Conclusions: Both products were classified as Non Irritant.

 

Citation Format: Rivero Y, González Y, Mancebo A, Scull I, Arteaga ME, González C, Bada AM (2015) Dermal and ocular irritation studies of two creams intended for its use for psoriasis. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S132. Abstract nr PTS-40.

PTS-39: CHRONIC ORAL TOXICITY OF TANNIN POWDERS OBTAINED FROM BARK OF Pinus caribaea MORELET IN RATS

J Pharm Pharmacogn Res 3(suppl. 1): S131, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PTS-39: CHRONIC ORAL TOXICITY OF TANNIN POWDERS OBTAINED FROM BARK OF Pinus caribaea MORELET IN RATS

Curbelo A1, Bada AM1, Santana JL2, González B1, González Y1, González C1, Arteaga ME1, Gómez D1, Mancebo A1.

1Centro Nacional para la Producción de Animales de Laboratorio (CENPALAB), La Habana, Cuba. E-mail: ailemys.curbelo@cenpalab.inf.cu
2Instituto Superior de Ciencias y Tecnologías Aplicadas (InsTEC), La Habana, Cuba.

 

Introduction: Effects of antimutagenic and non-genotoxic agents of tannins from Pinus caribaea Morelet, as well as its influence in improvement of quality life of patients have been demonstrated. Our aim was to assess chronic oral toxicity of tannin powder obtained from bark of P. caribaea in Cenp:SPRD rats. Material and Methods: Rats were randomly distributed into four experimental groups: Control, Low (1 mg/kg), Median (2 mg/kg) and High Dose (5 mg/kg). Administration was made daily along the six months of the study. Rats were inspected daily for clinical signs. Body weight and food and water consumption were weekly measured. Hematological and biochemical analysis were performed at the beginning, three months and at the end of experimentation. Gross necropsy and histological examination of tissues were performed on animals at the end of the assay. Results: During the study a female in Low Dose group died, the remainder finished the study in a good state. Clinical signs described were distributed by all groups and were considered not to have relation with the tested substance. Body weight curve was similar for all groups. Water consumption increased in animals treated with the higher dose, but the food consumption was normal. Clinical laboratory parameters had differences, although they were within normal ranks reported for the species. There were no macroscopic or microscopic lesions related with the tested substance. Conclusions: The tannin powder obtained from bark of P. caribaea appears to be safe under the experimental conditions established.

 

Citation Format: Curbelo A, Bada AM, Santana JL, González B, González Y, González C, Arteaga ME, Gómez D, Mancebo A (2015) Chronic oral toxicity of tannin powders obtained from bark of Pinus caribaea Morelet in rats. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S131. Abstract nr PTS-39.

PNAI-38: ANTIPLASMODIAL ACTIVITY AND ACUTE ORAL TOXICITY OF HYDROALCOHOLIC EXTRACTS OF Zuelania guidonia

J Pharm Pharmacogn Res 3(suppl. 1): S130, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-38: ANTIPLASMODIAL ACTIVITY AND ACUTE ORAL TOXICITY OF HYDROALCOHOLIC EXTRACTS OF Zuelania guidonia

Puente E1, Mora C2, Jaramillo M2, Berenguer C1, Rodríguez E1, Salas I1, Reinert A1, Cedeño N1.

1Centro de Toxicología y Biomedicina (TOXIMED), Autopista Nacional Km. 1 ½. Apartado: 4033, Santiago de Cuba. Código Postal: 90400. Cuba. E-mail: zapata.puente@medired.scu.sld.cu
2Facultad de Química Farmacéutica, Universidad de Antioquia, Colombia.

 

Introduction: According to estimates by the World Health Organization (WHO), malaria is a disease caused by parasites of the Plasmodium family. In 2013 it produced 198 million cases, which caused the death of about 584 000 people. The malaria mortality rate has dropped by over 47% since 2000 worldwide. A There is no licensed vaccine against malaria or other human parasite. Ethnobotanical studies showed data on the Zuelania guidonia and antiparasitic activity in areas of Cuba and Central America. Considering the biological evaluation performed raised two hydroalcoholic extracts vs Plasmodium, in vitro plant and acute oral toxicity thereof. Material and Methods: Extracts were obtained by the Soxhlet method, using as raw material to extract one leaves and bark extract two, a hydroalcoholic at 60 and 70 respectively menses. The antimalarial activity was performed by the method of continuous in vitro culture developed by Trager and Jensen (1976), chloroquine diphosphate (CQ) was used as positive control. To evaluate acute oral toxicity class method was used, following the rules of the OECD # 423 always starting dose of 300 mg/kg. Results: Evaluation in malaria Extract 1 was the active, with more than 45% of inhibition; the extract 2 had more than 18% inhibition. In acute oral toxicity they showed mortality rate of 300 < LD50 >2000 mg/kg. Conclusions: Thus it reaffirms that the formulations of the present antiplasmodial Zuelania guidonia toxicological activity and classified as dangerous in the animal model, dose levels employed and under the experimental conditions.

 

Citation Format: Puente E, Mora C, Jaramillo M, Berenguer C, Rodríguez E, Salas I, Reinert A, Cedeño N (2015) Antiplasmodial activity and acute oral toxicity of hydroalcoholic extracts of Zuelania guidonia. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S130. Abstract nr PNAI-38.

PNAI-37: In vitro ANTIFUNGAL ACTIVITY OF CRUDE HYDRO-ALCOHOLIC EXTRACT OF Petiveria alliacea L. ON CLINICAL Candida ISOLATES

J Pharm Pharmacogn Res 3(suppl. 1): S129, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-37: In vitro ANTIFUNGAL ACTIVITY OF CRUDE HYDRO-ALCOHOLIC EXTRACT OF Petiveria alliacea L. ON CLINICAL Candida ISOLATES

Velar RE1, Illnait MT1, Illnait J2, Fernández CM1, Martínez GF1, Perurena MR1, Monroy EX1, Meis JF3.

1Mycology Laboratory; Tropical Medicine Institute “Pedro Kourí” (IPK). Apartado Postal 601, Marianao 13, La Lisa. Havana, Cuba. Phone: 53-7-255 3524. Fax: 53-7-336048. E-mail: velar@ipk.sld.cu
2Natural Products Laboratory, National Centre for Scientific Research (CNIC). Ave. 25 and 158 Street, Apartado Postal 6412, Playa. Havana, Cuba. E-mail: jose.illnait@cnic.edu.cu
3Regional Public Health Laboratory Service. Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Hospital (CWZ), Weg door Jonkerbos 100 6532 SZ Nijmegen. P.O. Box 9015 6500 GS Nijmegen, The Netherlands. Phone: 31 243657514. E-mail: j.meis@cwz.nl

 

Introduction: The global burden of infections due to Candida and the emerging resistance to antifungals has led to the search for new therapeutic alternatives. The aim of the present work was to evaluate the in vitro antifungal activity of crude hydro-alcoholic extract of Petiveria alliacea L. (HAEPAL) versus fluconazole against Candida isolates. Methods: In vitro antifungal activity was evaluated by broth microdilution method in front of 125 Candida isolates (60 C. albicans). Inoculum of 1.5 x 106 CFU/mL in sterile saline solution was incubated with five dilutions of the extract (128, 64, 32, 16 and 8 μg/mL). The minimum inhibitory concentration (MIC) was defined as the lowest concentration of HAEPAL showing ≤50% growth compared with the extract-free growth control estimated by counting the CFU/mL. Fluconazole susceptibility was assessed by ATBTM Fungus 3 and E-test according to manufacturer’s instructions. Results: HAEPAL showed higher antifungal activity compared to fluconazole. Only four isolates (one C. albicans, one C. glabrata and two C. krusei) exhibited high MICs (≥ 64 μg/mL) compared to 34 (19 C. albicans and 15 Candida non-albicans), which were resistant to fluconazole. Conclusions: These results show the antifungal potentiality of HAEPAL, which could become a potential alternative for Candida treatment.

 

Citation Format: Velar RE, Illnait MT, Illnait J, Fernández CM, Martínez GF, Perurena MR, Monroy EX, Meis JF (2015) In vitro antifungal activity of crude hydro-alcoholic extract of Petiveria alliacea L. on clinical Candida isolates. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S129. Abstract nr PNAI-37.

PNAI-36: ANTIMICROBIAL ACTION OF Bixa orellana L.

J Pharm Pharmacogn Res 3(suppl. 1): S128, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-36: ANTIMICROBIAL ACTION OF Bixa orellana L.

Morales M1, Galdós MC1, López I1, Vázquez R2, Piña JC1.

1Camagüey Medical University. Carretera Central oeste Km 4 ½, Camagüey, Cuba. E-mail: marena@iscmc.cmw.sld.cu
2“Tte. Tomás Rojas” Teaching Polyclinic. Céspedes, Calle 31 Esquina 20, Camagüey, Cuba. E-mail: ramonvazquezgarcia69@yahoo.es

 

Introduction: Bixa orellana L. is a plant that grows in Cuba, to which medicinal properties are attributed. Material and Methods: An experimental study was carried out in order to determine the antimicrobial action of ethanolic extracts and dyes of B. orellana leaves against Candida albicans, Staphylococcus aureus, Escherichia coli, and Pseudomona aeruginosa. The macrodilution method was used and the concentration minimum germicide was determined expressed in dilutions. Stumps of international reference from American Type Culture Collection of E. coli, S. aureus, P. aeruginosa and C. albicans were used. Results: The dyes to 10% and 20% of the leaf showed inhibitory activity against S. aureus in dilutions up to 1:8 and 1:32 respectively; in E. coli; the dye to 10% presented activity up to 1:8, the dyes were not active against P. aeruginosa and C. albicans. The flowing extract to 50% showed inhibitory activity in front of S. aureus up to 1:4. The flowing extract to 50% and the dyes of the leaf to 10% and 20% was active in front of S. aureus to inferior concentrations with regard to the rest of the microorganisms, but they lacked antimicrobial activity in front of C. albicans and P. aeruginosa. Conclusions: S. aureus turned out to be the most sensitive germ to the pharmaceutical forms studied to smaller concentrations minimum germicides expressed in the dilutions. The dye to 10% showed a bigger spectrum of antimicrobial activity. The dye to 20% was the most effective.

 

Citation Format: Morales M, Galdós MC, López I, Vázquez R, Piña JC (2015) Antimicrobial action of Bixa orellana L. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S128. Abstract nr PNAI-36.

OC-29: ANTIMALARIAL USE VALIDATION OF PLANT SPECIES IN CUBA

J Pharm Pharmacogn Res 3(suppl. 1): S48, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-29: ANTIMALARIAL USE VALIDATION OF PLANT SPECIES IN CUBA

Fernández-Calienes A1, Fraga J1, Mendiola J1, Scull R2, Cuéllar A2, Bécquer E3, Herrera PP4.

1Departamento de Parasitología, Instituto de Medicina Tropical “Pedro Kourí” (IPK), Autopista Novia del Mediodía Km 6 ½, La Lisa, La Habana, Cuba. E-mail: ayme@ipk.sld.cu
2Instituto de Farmacia y Alimentos. Universidad de La Habana. Calle 222 N° 2317 entre 23 y 31. La Coronela. La Lisa. La Habana, Cuba.
3Jardín Botánico Nacional de Cuba, La Habana, Cuba.
4Instituto de Ecología y Sistemática, La Habana, Cuba.

 

Introduction: Malaria is the main parasitic disease causing high mortality and morbidity each year. Parasite resistance to current drugs threats malaria control. Based on traditional use and ethnomedical data, quina alkaloids and artemisinin were discovered in the past. Important ethnobotanical studies reveal traditional malaria treatments performed in Cuba before the disease eradication. This work is an attempt to become available a list of plants used empirically to combat malaria in Cuba, to summarise published data regarding the demonstration of their antimalarial activity and to identify natives species with antiplasmodial activity. Methods: A non-experimental validation of ethnomedical practices was aimed to point out traditional and probably effective treatments; three levels of validation were established: 1) for Cuban use only; 2) for uses recorded in other countries and 3) for positive antimalarial test. Antiplasmodial activity of 31 ethanolic extract was tested in vitro against Plasmodium berghei. Results: The use of 63 plant species for malaria, intermittent fevers and as quinine substitute were identify. Most represented family was Asteraceae, the majority of species were natives but only four were endemics. Decoction of leaves was the most frequent mode of remedy preparation and higher number of species was used in Eastern Cuba. Similar uses in other countries and/or positive screening against malaria parasites were reported for 36 of the 63 species. The medicinal use of 24 species possesses level 3 of non-experimental validation. Twenty-two extracts from 19 species (13 with level 3, 1 with level 2 and 5 with level 1) exhibited schizonts formation inhibition values upper than 50% at 100 µg/mL. Native species Baccharis halimifolia, Colubrina arborescens, Koanophyllon villosum, Oxandra lanceolata and Scutellaria havanensis demonstrated antiplasmodial activity for first time worldwide. Conclusions: Non-experimental validation and preliminary antiplasmodial test results substantiated the antimalarial use in Cuba for 30 plant species.

Citation Format: Fernández-Calienes A, Fraga J, Mendiola J, Scull R, Cuéllar A, Bécquer E, Herrera PP (2015) Antimalarial use validation of plant species in Cuba. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S48. Abstract nr OC-29.

PTS-43: ASSESSMENT OF ACUTE AND SUBCHRONIC ORAL TOXICITY OF SEED OIL Carapa guianensis Aublet EXTRACT

J Pharm Pharmacogn Res 3(suppl. 1): S135, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PTS-43: ASSESSMENT OF ACUTE AND SUBCHRONIC ORAL TOXICITY OF SEED OIL Carapa guianensis Aublet EXTRACT

Curveco D1, Arencibia D2, Rosario L3, Narciandi J3, Alonso A3, Crespo Y3, Vidal A4.

1Finlay Institute. 198 street and 27 avenue. Number 19805. La Lisa. Havana. Cuba. eneidasanchez@infomed.sld.cu
2CICA. 28 street between 5ta and 7ma Ave Number 502. Miramar, Playa Havana. Cuba.
3Institute of Pharmacy and Food (IFAL, U.H). 222 street between 25 and 27 avenue, La Lisa, Havana. Cuba.
4University of Havana, Biology Faculty. 25 avenue between I and J str.. Havana, Cuba.

 

Introduction: The seed oil extract of Carapa guianensis has various biomedical applications as solar protector, anticancer, antioxidant and antigenotoxic. The aim of this work was determined toxic effects of oil extract orally administered to Sprague Dawley rats from both sexes. Methods: Oral acute toxicity (2 000 mg/kg) was investigated according to the Acute Toxic Class Method and the subchronic study, rats were treated with doses at 400, 1 000 and 2 000 mg/kg for 90 days. Results: No evidence of treatment-related toxicity was detected. Thus, clinical observations, body weight, food intake, haematological parameters, organ weight ratios and histopathological findings showed no significant differences between control and treated groups. However, 2000 mg/kg in subchronic study, only in male rats, evidenced smaller increment in liver weight and alanine aminotransferase (ALT). Conclusions: These studies showed no evidences related oral toxicity in the administration of Carapa guianensis oil extract. It is recommended hepatic toxicity studies to demonstrate the mechanism that leads to an increase in liver enzymes in the highest dose evaluated.

 

Citation Format: Curveco D, Arencibia D, Rosario L, Narciandi J, Alonso A, Crespo Y, Vidal A (2015) Assessment of acute and subchronic oral toxicity of seed oil Carapa guianensis Aublet extract. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S135. Abstract nr PTS-43.

PTS-42: TOXICOLOGICAL STUDIES TO THE PRODUCTS CODASAL PLUS, CODAHUMUS 20, CODACÍTRICOS, CODAMIN RADICULAR, CODAMIN B-Mo AND CODAN

J Pharm Pharmacogn Res 3(suppl. 1): S134, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PTS-42: TOXICOLOGICAL STUDIES TO THE PRODUCTS CODASAL PLUS, CODAHUMUS 20, CODACÍTRICOS, CODAMIN RADICULAR, CODAMIN B-Mo AND CODAN USED IN THE AGRICULTURE AS BIO-FERTILIZERS

García G, Casanova M, Jorge N, Pardo GL.

Center of Study for the Investigations and Biological Evaluations (CEIEB), Institute of Pharmacy and Foods, University of Havana, Cuba. E-mail ggarcia@ifal.uh.cu

 

Introduction: The products CODA from Sustainable Agrosolutions SA (CodasalPlus, Codahumus 20, Codacítricos, Codamin Radicular, Codamin B-Mo, and Codan) are composed by a natural mixture of amino acids, and biostimulant sand are used to get maximum yields and qualities of crop growing by improving earth and water. In this study we carried out the first barrier toxicity assays for these products: Material and Methods: It were used the norms described in the Technical Guidelines of the Organization for the Cooperation and Development (OECD), and other reported by the International Organization of Standardization (ISO) for tests of Sensitization, Oral and Dermal Acute Toxicity, and Ocular and Dermal Irritability. Results: The results indicated that the above mentioned products are not sensitizing agents. In the case of Ocular Irritation test, only the Codacítricos was rejected do to its toxicity to ocular structures. In relation to the Dermal Irritation, the index or punctuation was 0, indicating the dermal safeness of the bio-fertilizers. Concerning the Oral Acute Toxicity test, all the products analyzed passed the test. Finally, regarding the Dermal Acute Toxicity test, visible damages were localized in the skin, but non deaths except in the Codahumus 20 were observed. In this case, it was necessary to reduce the dose. Conclusions: These products can be used and manipulated by humans, providing the suitable cautions emanated from this study.

 

Citation Format: García G, Casanova M, Jorge N, Pardo GL (2015) Toxicological studies to the products Codasal Plus, Codahumus 20, Codacítricos, Codamin Radicular, Codamin B-MO and Codan used in the agriculture as bio-fertilizers. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S134. Abstract nr PTS-42.