Category Archives: Inflammation

Anti-inflammatory effect of amitriptyline

J Pharm Pharmacogn Res 5(3): 144-155, 2017.

Original Article | Artículo Original

In vitro and in vivo modulation of LPS and carrageenan-induced expression of inflammatory genes by amitriptyline

[Modulación in vitro e in vivo por amitriptilina de la expresión de genes inflamatorios inducidos por LPS y carragenina]

Laleh Rafiee1, Valiollah Hajhashemi2*, Shaghayegh Haghjooy Javanmard3

1Applied Physiology Research Center, Isfahan Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
2Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
3Applied Physiology Research Center, Isfahan Cardiovascular Research Institute and Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.

*E-mail: vhajhashemi@gmail.com

Abstract

Context: Amitriptyline, a tricyclic antidepressant is used for the management of psychological disorders and various types of pain. In the previous work, it is founded that amitriptyline inhibited the migration of polymorphonuclear (PMN) into the site of inflammation.

Aims: To evaluate the effect of amitriptyline on the expression of some inflammatory mediators such as intercellular adhesion molecule (ICAM-1), vascular cell adhesion molecule (VCAM-1), cyclooxygenase 2 (COX2) and inducible nitric oxide synthase (iNOS).

Methods: An in vitro model system of LPS-stimulated human endothelial cells and U937 macrophages and also in vivo model of carrageenan-induced paw edema in rat were used. The expression of inflammatory mediator genes was determined by qRT-Real-time PCR. In endothelial cells, soluble forms of ICAM-1 and VCAM-1 were quantified by ELISA.

Results: The expression of ICAM-1, VCAM-1, COX2, iNOS, sICAM-1 and sVCAM-1 significantly decreased by amitriptyline. The finding of this study also confirmed that intraperitoneal (i.p.) injection of amitriptyline inhibited carrageenan-induced inflammation in rat paw edema.

Conclusions: The results of the present study provide further evidence for the anti-inflammatory effect of amitriptyline. This effect appears to be mediated by down-regulation of inflammatory genes.

Keywords: amitriptyline; cyclooxygenase 2; inducible nitric oxide synthase; inflammation; intercellular adhesion molecule-1; vascular cell adhesion molecule-1.

Resumen

Contexto: La amitriptilina, un antidepresivo tricíclico, se utiliza para el tratamiento de los trastornos psicológicos y diversos tipos de dolor. En un trabajo anterior se demostró el efecto inhibidor de la amitriptilina sobre la migración de polimorfonucleares (PMN) en el sitio de la inflamación.

Objetivos: Evaluar el efecto de la amitriptilina sobre la expresión de algunos mediadores inflamatorios tales como molécula de adhesión intracelular (ICAM-1), molécula de adhesión celular vascular (VCAM-1), ciclooxigenasa 2 (COX2) y óxido nítrico sintasa inducible (iNOS).

Métodos: Se utilizó un modelo in vitro de células endoteliales humanas estimuladas con LPS y macrófagos U937 y también un modelo in vivo de edema de la pata inducido por carragenina en rata. La expresión de genes mediadores inflamatorios se determinó por PCR qRT-Real-time. En las células endoteliales, las formas solubles de ICAM-1 y VCAM-1 se cuantificaron por ELISA.

Resultados: La expresión de ICAM-1, VCAM-1, COX2, iNOS, sICAM-1 y sVCAM-1 disminuyó significativamente por la amitriptilina. También se confirmó que la amitriptilina intraperitoneal (i.p.) inhibió la inflamación inducida por carragenina en el edema de la pata de la rata.

Conclusiones: Los resultados de este estudio proporcionan evidencia adicional para el efecto antiinflamatorio de la amitriptilina. Este efecto parece estar mediado por la regulación hacia abajo de los genes inflamatorios.

Palabras Clave: amitriptilina; ciclooxigenasa 2; inflamación; molécula de adhesión celular vascular-1; molécula de adhesión intracelular-1; óxido nítrico sintasa inducible.

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Citation Format: Rafiee L, Hajhashemi V, Haghjooy Javanmard S (2017) In vitro and in vivo modulation of LPS and carrageenan-induced expression of inflammatory genes by amitriptyline. J Pharm Pharmacogn Res 5(3): 144-155.

© 2017 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

Potentials of Bombax ceiba thorn extract

J Pharm Pharmacogn Res 5(1): 40-54, 2017.

Original Article | Artículo Original

Pharmacognostic and pharmacological studies of Bombax ceiba thorn extract

[Estudios farmacognóstico y farmacológico del extracto de espina de Bombax ceiba]

Manish A. Kamble, Debarshi Kar Mahapatra*, Disha M. Dhabarde, Ashwini R. Ingole

Department of Pharmacognosy, Kamla Nehru College of Pharmacy, Nagpur – 441108, Maharashtra, India.

*E-mail: dkmbsp@gmail.com

Abstract

Context: Bombax ceiba is a large deciduous tree found in tropical and sub-tropical regions of Asia, Africa, and Australia. Traditional systems of medicine such as Ayurveda, Siddha and Unani have been highlighted the use of B. ceiba parts (bark, leaves, and flower) for the treatment of numerous ailments like algesia, hepatotoxicity, hypertension, HIV infections, fever, dysentery, inflammation, catarrhal affection, ulcer, acne, gynecological disorders, piles and urinary infections. However, no scientific pharmacognostic, phytochemical and pharmacological study has been reported for B. ceiba thorn.

Aims: To study the pharmacognostic and pharmacological potentials of B. ceiba thorn extract.

Methods: The physicochemical properties were determined using pharmacopoeial tests. The phytochemical screening was carried out using standard protocols. The in vitro anti-inflammatory activity was performed by human red blood cells stabilization method, in vitro anthelmintic activity by Pheretima posthuma method, in vitro antioxidant activity by DPPH scavenging method, and anti-microbial studies by agar streak dilution method against bacteria E. coli, B. subtilis, K. pneumonia, and fungi C. albicans and A. niger.

Results: The hydroalcoholic thorn extract of Bombax ceiba (TEBC) showed significant anti-inflammatory (46.9% stabilization), anthelmintic (death at 59.22 min), in vitro anti-oxidant (41.62% inhibition), and anti-microbial activities (B. subtilis > E. coli > K. pneumonia; A. niger > C. albicans).

Conclusions: The study revealed the physicochemical, photochemical and pharmacognostic features of thorn of the B. ceiba. The study also revealed the possession of different pharmacological potentials of extract.

Keywords: anthelmintic; anti-inflammatory; antimicrobial; Bombax ceiba; pharmacognosy; thorn extract.

Resumen

Contexto: Bombax ceiba es un árbol de hoja caduca que se encuentra en las regiones tropicales y subtropicales de Asia, África, y Australia. Los sistemas tradicionales de la medicina como Ayurveda, Siddha y Unani han puesto de relieve el uso de las partes de B. ceiba (corteza, hojas y flores) para el tratamiento de numerosas enfermedades como la sensibilidad dolorosa, hepatotoxicidad, hipertensión, infecciones por VIH, fiebre, disentería, inflamación, catarros, úlceras, acné, trastornos ginecológicos, hemorroides y las infecciones urinarias. Sin embargo, no hay estudios científicos farmacognósticos, fitoquímicos y farmacológicos sobre la espina de B. ceiba.

Objetivos: Estudiar las potencialidades farmacognósticas y farmacológicas de extracto de espina de B. ceiba.

Métodos: Las propiedades fisicoquímicas se determinaron mediante pruebas reportadas en la farmacopea. El tamizaje fitoquímico se llevó a cabo utilizando protocolos estándar. La actividad anti- inflamatoria in vitro se realizó mediante el método de estabilización de glóbulos rojos humanos, la actividad antihelmíntica in vitro por el método de Pheretima posthuma, la actividad anti-oxidante in vitro por el método de secuestro de DPPH y los estudios anti-microbianos por el método de dilución en agar contra las bacterias E. coli, B. subtilis, K. neumonía y hongos C. albicans y A. niger.

Resultados: El extracto hidroalcohólico de espina de Bombax ceiba (TEBC) mostró significativa actividad anti- inflamatoria (46.9% de estabilización), vermífuga (muerte en 59.22 min), anti-oxidante (41.62% de inhibición) y anti-microbiana (B. subtilis > E. coli > K. pneumoniaA. niger > C. albicans).

Conclusiones: El studio reveló las características fisicoquímicas, fitoquímicas y farmacognósticas de la espina de B. ceiba. También reveló las potencialidades farmacológicas del extracto.

Palabras Clave: anti-inflamatorio; antimicrobiano; Bombax ceiba; extracto de la espina; farmacognosia; vermífugo.

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Citation Format: Kamble MA, Mahapatra DK, Dhabarde DM, Ingole A (2017) Pharmacognostic and pharmacological studies of Bombax ceiba thorn extract. J Pharm Pharmacogn Res 5(1): 40-54.
This article has been cited by:
Borikar SP, Kallewar NG, Mahapatra DK, Dumore NG (2018) Dried flower powder combination of Clitoria ternatea and Punica granatum demonstrated analogous anti-hyperglycemic potential as compared with standard drug metformin: In vivo study in Sprague Dawley rats. Journal of Applied Pharmaceutical Science 8(11): 75-79. DOI: 10.7324/JAPS.2018.81111
Joseph TM, Mahapatra DK (2018) Evaluating the antibacterial perspective of cuminaldehyde, a component of Cuminum cyminum against some common human intestinal pathogens. Research & Reviews: Journal of Herbal Science 7(2): 10-12.  Website
Mahapatra DK, Shivhare RS (2018) Anti-microbial perspective of a chalcone, (E)-1-(1- methoxy-9H-carbazol-3-yl)-3-(4-(methylsulfonyl) phenyl)prop-2-en-1-one: Fabrication of a hybrid by unification of a natural product with a synthetic component. Int J Agr Life Sci 4(2): 236-240.DOI: 10.22573/spg.ijals.018.s12200090
Borikar SP, Kallewar NG, Mahapatra DK, Gupta RA, Dumore NG, Danao KR, Mahajan UN (2018) Decoction of Andrographis paniculata whole plant and Gymnema sylvestre leaves demonstrated noteworthy hypoglycemic activity in Sprague Dawley rat. J Phytopharmacol 7(1):84-87. Website
Telrandhe R, Mahapatra DK, Kamble MA (2017) Bombax ceiba thorn extract mediated synthesis of silver nanoparticles: Evaluation of anti-Staphylococcus aureus activity. International Journal of Pharmaceutics & Drug Analysis 5(9): 376–379. Website

© 2017 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

BPH treatment with the natural product Calprost®

J Pharm Pharmacogn Res 4(5): 187-198, 2016.

Original Article | Artículo Original

Clinical evaluation of patients with benign prostatic hyperplasia, treated with the natural product Calprost®: a randomized, controlled study

[Evaluación clínica de pacientes con hiperplasia prostática benigna, tratados con el producto natural Calprost®: estudio aleatorizado, controlado]

Magnelis Machado-Leiva1, Daise Jiménez-Rodríguez2, Tatiana Festary-Casanovas2, Deydree  C. Silveira-Pacheco3, Emilio S. Barroso-de la Cruz4, Mariela Suárez-Reyes1, Genma Salas-Cruz1, Haydee Wong-Arocha3, Roberto Fernández-Viera4, Ángela D. Tuero-Iglesias5, René Delgado-Hernández2, Idrian García-García2*, for the Calprost Study Group

1Urology and Natural and Traditional Medicine Services, Clinical-Surgical Hospital “Dr. Luis Díaz Soto”, Havana, Cuba
2Clinical Trials Group, Research Direction, Center for Drug Research and Development, Ave. 26 and Puentes Grandes, No. 1605, Nuevo Vedado,Havana, Cuba
3Urology Service, Clinical-Surgical Hospital “Joaquín Albarrán”, Havana, Cuba
4Urology Service, Clinical-Surgical Hospital “Iván Portuondo”, San Antonio de los Baños, Artemisa, Cuba
5Center for Genetic Engineering and Biotechnology, Havana, Cuba.

*E-mail: idrian.garcia@cidem.cu

Abstract

Context: Benign Prostatic Hyperplasia (BPH) is a common disease that course with Lower Urinary Tract Symptoms (LUTS), mainly in over 50 years-old men. Commonly indicated drugs such as alpha adrenergic-blockers are life-treatment with some adverse reactions. Center for Drug Research and Development produce a microencapsulated lipophilic extract of pumpkin seed oil (Calprost®) with anti-androgenic, anti-inflammatory, antioxidant, antiproliferative and diuretic properties.

Aims: To evaluate the effect and safety of Calprost® in patients with BPH and LUTS.

Methods: A multicenter, randomized, controlled, open exploratory clinical trial was conducted. Two experimental groups, study group (Calprost®, 140 mg daily) (n=81), and control group (terazosin, 2 mg daily) (n=50) were conformed. All the patients were treated during three months. Efficacy was evaluated through International Prostate Symptoms Score (IPSS), residual bladder volume and prostate volume.

Results: Most of the included patients (74.0%) were white skin color and their mean age was 66 yrs. Fifteen patients, nine of them from terazosin group, withdraw the trial voluntarily. A significant reduction in the overall IPSS scale was obtained for both groups. Nevertheless, some obstructive (intermittency, straining) and irritative (frequency, urgency) urinary symptoms decreased more markedly in the Calprost® group being milder. Median residual and prostatic volumes decreased significantly (p=0.048 and p=0.002, respectively) only into the Calprost® group. Most of the adverse events were recorded in the terazosin group (79.4%), where postural hypotension prevailed.

Conclusions: The natural product Calprost® was probed as a successful treatment of patients with BPH/LUTS, being also well-tolerated.

Keywords: Benign prostatic hyperplasia; Calprost®; IPSS; lower urinary tract symptoms, pumpkin seed oil, terazosin.

Resumen

Contexto: La Hiperplasia Prostática Benigna (HPB) es una enfermedad común en hombres mayores de 50 años de edad, que cursa con Síntomas del Tracto Urinario Bajo (STUB). Los tratamientos de por vida con medicamentos bloqueadores alfa-adrenérgicos producen algunas reacciones adversas. El Centro de Investigación y Desarrollo de Medicamentos produce un extracto lipofílico microencapsulado de aceite de semillas de calabaza (Calprost®) con propiedades anti-androgénicas, anti-inflamatorias, antioxidantes, antirproliferativas y diuréticas.

Objetivos: Evaluar el efecto  y seguridad del Calprost® en pacientes con BPH/STUB.

Métodos: Se realizó un  estudio exploratorio, multicéntrico, aleatorizado, controlado y abierto. Se conformaron dos grupos experimentales, estudio (Calprost®, 140 mg diarios) (n=81), y control (terazosina, 2 mg diarios) (n=50). Todos los pacientes fueron tratados durante tres meses. La eficacia se evaluó mediante la sintomatología urinaria (escala IPSS), volumen vesical residual y volumen prostático.

Resultados: La mayoría de los pacientes (74.0%) fueron blancos, con edad promedio de 66 años. Quince pacientes, nueve de ellos del grupo terazosina, abandonaron el estudio voluntariamente. Hubo disminución significativa de la escala global de síntomas en ambos grupos, aunque algunos de ellos se redujeron notablemente solo en el grupo Calprost®, llegando a ser más ligeros. La mediana tanto del volumen residual (p=0.048) como del prostático (p=0.002) disminuyó de manera significativa solo en el grupo tratado con Calprost®. El porcentaje de eventos adversos presentados fue mayor con la terazosina (79.4%), prevaleciendo la hipotensión postural.

Conclusiones: El producto natural Calprost® fue efectivo en el tratamiento de la HPB/STUB, siendo además bien tolerado.

Palabras Clave: Aceite de semilla de calabaza; Calprost®; hiperplasia prostática benigna; IPSS; síntomas del tracto urinario bajo; terazosina.

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Citation Format: Machado-Leiva M, Jiménez-Rodríguez D, Festary-Casanovas T, Silveira-Pacheco DC, Barroso-de la Cruz ES, Suárez-Reyes M, Salas-Cruz G, Wong-Arocha H, Fernández-Viera R, Tuero-Iglesias AD, Delgado-Hernández R, García-García Idrian, for the Calprost Study Group (2016) Clinical evaluation of patients with benign prostatic hyperplasia, treated with the natural product Calprost®: a randomized, controlled study.J Pharm Pharmacogn Res 4(5): 187-198.

© 2016 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

Leucas aspera apigenin glucopyranoside: anti-inflammatory and wound healing activities

J Pharm Pharmacogn Res 4(2): 54-61, 2016.

Original Article | Artículo Original

Isolation of apigenin-7-O-(6’’-O-E-caffeoyl)-β-D-glucopyranoside from Leucas aspera L. with anti-inflammatory and wound healing activities

[Aislamiento de apigenina-7-O-(6’’-O-E-cafeoil)-β-D-glucopiranósido de Leucas aspera L. con actividad anti-inflamatoria y cicatrizante]

Rajamanickam Manivannan*

Department of Chemistry, Government Arts College (Autonomous), Kumbakonam, Tamilnadu-612001, India.
Abstract

Context: Leucas aspera L. (Labiatae) is a common aromatic herb and grows generously in South India and in the broad area of South Asia. Traditionally, this species is taken orally for analgesic, anti-inflammatory, anti-bacterial and wound healing treatments.

Aims: To isolate compounds from L. aspera with anti-inflammatory and wound healing activities.

Methods: The chloroform extract was subjected to a column chromatography on silica gel 60 and their structures were established by spectral analysis (UV, IR, and NMR). The anti-inflammatory activity of the test compounds was evaluated in male albino rats. The acute inflammation was induced by the subplantar administration of 0.1 mL of 1% carrageenan in the right paw. The excision wound model was used to study the rate of wound contraction and the time required for complete epithelization of the injuries in rabbits.

Results: A flavonoid apigenin-7-O-(6”-O-E-caffeoyl)-β-D–glucopyrano-side (1) was isolated from a chloroform fraction of L. aspera. The hydrolysis of compound 1 yield an apigenin (aglycone), caffeic acid and β-D-glucose. Assuming caffeoyl glucose linked to the 7-OH group of apigenin.

Conclusions: Compound 1 exhibited a significant anti-inflammatory activity compared with standard diclofenac sodium. The wound healing study revealed that decreased wound area and significant increase in epithelialization in treatment groups was observed.

Keywords: Anti-inflammatory; flavonoids; Leucas aspera; wound healing.

Resumen

Contexto: Leucas aspera L. (Labiatae) es una hierba aromática común y crece generosamente en el sur de la India y en la zona amplia del sur de Asia. Tradicionalmente, esta especie se toma por vía oral para tratamientos analgésicos, anti-inflamatorios, anti-bacterianas y la cicatrización de heridas.

Objetivos: Aislar compuestos de L. aspera con actividades anti-inflamatoria y cicatrizante.

Métodos: El extracto clorofórmico de L. aspera se sometió a una columna cromatográfica de sílica gel 60 y las estructuras fueron establecidas por análisis espectral (UV, IR y RMN). La actividad anti-inflamatoria de los compuestos estudiados fue evaluada en ratas albinas machos. La inflamación aguda fue inducida por la administración subplantar de 0,1 mL de carragenano 1% en la aponeurosis plantar derecha. El modelo de escisión de la herida fue usado para estudiar la tasa de contracción de la herida y el tiempo requerido para la completa epitelización de las heridas en conejos.

Resultados: De la fracción clorofórmica de L. aspera se aisló un flavonoide apigenina-7-O-(6”-O-E-cafeoil)-β-D-glucopiranósido (1). La hidrólisis del compuesto 1 rindió apigenina (aglicona), ácido cafeico y β-D-glucosa. Suponiendo que la cafeoil-glucosa estaba unida al grupo 7-OH de apigenina.

Conclusiones: El compuesto 1 exhibió una actividad anti-inflamatoria significativa en comparación con diclofenaco sódico estándar. El estudio de la curación de las heridas reveló que hubo una disminución del área de la herida y un aumento significativo en la epitelización en los grupos de tratamiento.

Palabras Clave: Anti-inflamatorio; cicatrización de herida; flavonoides; Leucas aspera.

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Citation Format: Manivannan R (2016) Isolation of apigenin-7-O-(6’’-O-E-caffeoyl)-β-D-glucopyranoside from Leucas aspera L. with anti-inflammatory and wound healing activities. J Pharm Pharmacogn Res 4(2): 54-61.
This article has been cited by:
Setzer WN (2018) The phytochemistry of cherokee aromatic medicinal plants. Medicines 5: 121. DOI: 10.3390/medicines5040121

© 2016 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

Actividad anti-inflamatoria de Tabebuia hypoleuca

J Pharm Pharmacogn Res 3(5): 109-117, 2015.

Original Article | Artículo Original

Actividad anti-inflamatoria de los extractos metanólicos de hojas y de tallos de Tabebuia hypoleuca (C. Wright) Urb.

[Anti-inflammatory activity of the methanolic extracts of leaves and stems from Tabebuia hypoleuca (C. Wright) Urb.]

Ada I. Regalado*, Luz M. Sánchez, Betty Mancebo

Departamento de Química Farmacología Toxicología. Centro Nacional de Sanidad Agropecuaria (CENSA). PO Box 10, San José de las Lajas, Mayabeque, CP 32700, Cuba.
Abstract

Context: There are reports in the literature of species belonging to the genus Tabebuia with pharmacological potential as anti-inflammatory: Tabebuia avellanedae, Tabebuia chrysanta, Tabebuia rosea, Tabebuia ochracea, among others; however, about of the species Tabebuia hypoleuca no studies demonstrating this activity so far.

Aims: To determine the anti-inflammatory activity in the acute phase of the methanolic extracts of T. hypoleuca.

Methods: Leaves and stems of T. hypoleuca were collected. The anti-inflammatory activity was assessed using the carrageenin-induced paw edema models and the croton oil induced auricular edema in mice. The qualitative identification of secondary metabolites present in the methanolic extracts was performed by a preliminary phytochemical screening.

Results: The anti-inflammatory activity assessments showed that methanol extract of the leaves do not have anti-inflammatory activity at doses tested, while the methanol extract of the stems at the dose of 500 mg/kg showed a significant anti-inflammatory activity in the model of carrageenan-induced paw edema. In the model of croton oil induced auricular edema the methanol extract of the stems administered orally and intraperitoneally showed a significant anti-inflammatory activity at all doses tested. The anti-inflammatory activity found could be due to the presence of metabolites such as tannins, phenols and alkaloids.

Conclusions: These studies demonstrate the anti-inflammatory activity of the methanol extract of the stems of Tabebuia hypoleuca, and constitute the first report about this species as anti-inflammatory.

Keywords: Anti-inflammatory; carrageenan; croton oil; phytochemical screening; Tabebuia hypoleuca.

Resumen

Contexto: Existen reportes en la literatura de especies que pertenecen al género Tabebuia con potencial farmacológico como anti-inflamatorio: Tabebuia avellanedae, Tabebuia chrysanta, Tabebuia rosea, Tabebuia ochracea, entre otras; sin embargo, de la especie Tabebuia hypoleuca no se encuentran estudios hasta el momento que demuestren esta actividad.

Objetivos: Determinar la actividad anti-inflamatoria en la fase aguda de los extractos metanólicos de T. hypoleuca.

Métodos: Hojas y tallos de T. hypoleuca fueron colectados. La actividad anti-inflamatoria se evaluó utilizando los modelos de edema plantar por carragenina y el edema auricular por aceite de croton en ratones. Se realizó la identificación cualitativa de los metabolitos secundarios presentes en los extractos metanólicos mediante un tamizaje fitoquímico preliminar.

Resultados: Las evaluaciones de la actividad anti-inflamatoria mostraron que el extracto metanólico de las hojas no posee actividad anti-inflamatoria a ninguna de las dosis evaluadas, mientras que el extracto metanólico de los tallos a la dosis de 500 mg/kg, mostró una actividad anti-inflamatoria significativa en el modelo de edema plantar por carragenina. En el modelo de edema auricular por aceite de croton el extracto metanólico de los tallos, administrado por vía oral e intraperitoneal, mostró una actividad antiinflamatoria significativa en todas las dosis evaluadas. La actividad anti-inflamatoria encontrada podría deberse a la presencia de metabolitos como taninos, fenoles y alcaloides.

Conclusiones: Estos estudios demuestran la actividad anti-inflamatoria del extracto metanólico de los tallos de Tabebuia hypoleuca y constituyen el primer reporte dado a la especie como anti-inflamatorio.

Palabras Clave: Aceite de croton; anti-inflamatorio; carragenina; Tabebuia hypoleuca; tamizaje fitoquímico.

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Citation Format: Ada I. Regalado, Luz M. Sánchez, Betty Mancebo (2015) Actividad anti-inflamatoria de los extractos metanólicos de hojas y de tallos de Tabebuia hypoleuca (C. Wright) Urb. [Anti-inflammatory activity of the methanolic extracts of leaves and stems from Tabebuia hypoleuca(C. Wright) Urb.]. J Pharm Pharmacogn Res 3(5): 109-117.

 

This article has been by:
Ada I. Regalado, Betty Mancebo, Armindo Paixão, Luz M. Sánchez (2017) Evaluation of antipyretic, sedative and hypnotic activities of methanol extract of Tabebuia hypoleuca (C. Wright ex Sauvalle) Urb. stems. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 16 (6): 547 - 555. Website
Ada I. Regalado, Betty Mancebo, Armindo Paixão, Yanet López, Nelson Merino, Luz M. Sánchez (2017) Antinociceptive activity of methanol extract of Tabebuia hypoleuca (C. Wright ex Sauvalle) Urb. stems. Medical Principles and Practice 26(4): 368-374. DOI: 10.1159/000478015

© 2015 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

CO 069: LEVELS OF SOLUBLE IL-15, IL-6, TNFΑ AND IL-15RΑ IN SYNOVIAL FLUID AND MEMBRANE BOUND IL-15 ON SYNOVIAL CELLS FROM PATIENTS WITH RHEUMATOID ARTHRITIS AND OSTEOARTHRITIS

J Pharm Pharmacogn Res 2(Suppl. 1): S42, 2014

Special supplement with the abstract book of LATINFARMA 2013

Oral Communication

CO 069: LEVELS OF SOLUBLE IL-15, IL-6, TNFΑ AND IL-15RΑ IN SYNOVIAL FLUID AND MEMBRANE BOUND IL-15 ON SYNOVIAL CELLS FROM PATIENTS WITH RHEUMATOID ARTHRITIS AND OSTEOARTHRITIS

Machado-Díaz AC1, Arrieta-Aguero C1, Chico-Capote A2, RodriguezAlvarez Y1, García-del Barco Herrera D1, Raices-Cruz I1, Falcón-Cama V1, Chacón-Quintero Y1, Guillen-Nieto G1, Santos-Savio A1.

1Centro de Ingeniería Genética y Biotenología. La Habana, Cuba. E-mail: ana.machado@cigb.edu.cu
2Hospital Hermanos Amejeiras. La Habana, Cuba.
Abstract

Introduction: Cytokines are a family of proteins involved in regulation of the immune system and the intercellular communication. Many autoimmune diseases such as Rheumatoid arthritis (RA) are related to deregulation of cytokine expression. High levels of pro-inflammatory cytokines IL-15, IL-6 and TNF-α have been described in synovial fluid from RA patients.

Material and methods: In this study we quantified these cytokines levels in synovial fluid from 18 RA and 17 osteoarthritis (OA) patients, from Rheumatology service at B. Ameijeiras hospital, who presented inflammation and abundant synovial fluid in damaged joints. We also quantified levels of soluble IL-15Rα, a private receptor for IL-15, using an ELISA designed by our group. We also studied expression of membrane-bound IL-15 on synovial cells by FACS and EM.

Results: Current study confirmed presence of soluble IL-15Rα in synovial fluids from RA and OA patients. Although we detected expression of membrane IL-15 on cells from synovial fluids of RA patients. Also we determined that IL-15 is present as a membrane bound ligand. Acidic treatment produced a slight decrease in the amount of membrane-bound IL-15. Indicating that, in addition to transmembrane IL-15, a certain number of IL-15 molecules are bound to membrane IL-15Rα. Interestingly, we found a positive correlation between high levels of IL-6 and high levels of IL-15Rα in RA but not in OA.

Conclusions: The presence of soluble IL-15Rα and membrane IL-15 suggested that soluble IL-15Rα could induce IL-6 through a reverse signaling pathway and then contributing to a proinflammatory medium in RA.

CO 068: THE LONG PENTRAXIN PTX3: A NON-REDUNDANT COMPONENT OF THE HUMORAL INNATE IMMUNITY AND A PROMISING BIOMARKER IN INFLAMMATORY CONDITIONS

J Pharm Pharmacogn Res 2(Suppl. 1): S42, 2014

Special supplement with the abstract book of LATINFARMA 2013

Oral Communication

CO 068: THE LONG PENTRAXIN PTX3: A NON-REDUNDANT COMPONENT OF THE HUMORAL INNATE IMMUNITY AND A PROMISING BIOMARKER IN INFLAMMATORY CONDITIONS

Garlanda C, Jaillon S, Barbati E, Bottazzi B, Mantovani A.

Humanitas Clinical and Research Center, via Manzoni 113, Rozzano, 20089, Italy. E-mail: cecilia.garlanda@humanitasresearch.it
Department of Translational Medicine, University of Milan, Milan, Italy.
Abstract

Pentraxins are a family of evolutionarily conserved multifunctional pattern-recognition proteins characterized by a cyclic multimeric structure. Based on the primary structure of the subunit, the pentraxins are divided into two groups: short pentraxins and long pentraxins. C-reactive protein (CRP) and serum amyloid P-component (SAP) are the two short pentraxins. The prototype protein of the long pentraxin group is pentraxin 3 (PTX3). CRP and SAP are produced primarily in the liver in response to IL-6, while PTX3 is produced by a variety of tissues and cells and in particular by innate immunity cells in response to pro-inflammatory signals and Toll-like receptor (TLR) engagement.

Through in vitro and in vivo studies performed with original tools generated by this group (recombinant human and mouse PTX3, its domains and mutated variants, original anti-PTX3 antibodies and PTX3 gene targeted mice), it has been shown that PTX3 interacts with several ligands, including growth factors, extracellular matrix components and selected pathogens, playing a role in complement activation and pathogen recognition by phagocytes, in tuning inflammation and in tissue remodelling. In addition, data obtained so far with ELISA assays on human plasma or serum suggest that PTX3 may represent a useful marker of different inflammatory conditions including cardiovascular pathology complementary to CRP: being directly produced by damaged tissues, its increase precedes CRP and rapidly reflects the vascular involvement by inflammatory process. The combination of PTX3 and classical biomarkers showed an incremental diagnostic and prognostic value in several conditions, including sepsis, acute coronary syndromes and chronic heart failure.

Thus, the prototypic long pentraxin PTX3 is a multifunctional soluble pattern recognition receptor acting as a non-redundant component of the humoral arm of innate immunity and a novel promising biomarker to provide useful prognostic information for clinical outcomes in inflammatory conditions.

SEPSIS, INFLAMMATION AND CELL RESPONSE

J Pharm Pharmacogn Res 2(Suppl. 1): S77, 2014

Special supplement with the abstract book of LATINFARMA 2013

Plenary Lecture

PL 007: SEPSIS, INFLAMMATION AND CELL RESPONSE

Salomao R.

Laboratório de Imunologia Universidade Federal de São Paulo/Escola Paulista de Medicina. Rua Pedro de Toledo, 781 – 15º andar – Vila Clementino. São Paulo, Brasil.
Abstract

Since the definition of systemic inflammatory response syndrome/sepsis was originally proposed, a large amount of new information has been generated showing a much more complex scenario of inflammatory and counter inflammatory responses during sepsis. Moreover, some fundamental mechanisms of sensing and destroying invading microorganisms have been uncovered, which include the discovery of TLR4 as the lipopolysaccharide (LPS) gene, implications of innate immune cells as drivers of the adaptive response to infection, and the modulation of multiple accessory molecules that stimulate or inhibit monocyte/macrophage and lymphocyte interactions. The complexity of the infection/injury-induced immune response could be better appreciated with the application of genomics and proteomics studies, and LPS was a useful tool in many of these studies. In this review, we discuss aspects of bacterial recognition and induced cellular activation during sepsis. Because of the relevance of endotoxin (LPS) research in the field, we focus on LPS and host interactions as a clue to understand microorganisms sensing and cell signaling, then we discuss how this response is modulated in septic patients.