Category Archives: Ozone

Facial biostimulation with PRP in HIV patients

AIDS, Antioxidant, biochemical markers, Clinical Pharmacology, Clinical Trial, HIV, Oxidative Stress, oxidative stress markers, Ozone
J Pharm Pharmacogn Res 7(4): 273-287, 2019.

Original Article | Artículo Original

Facial biostimulation with PRP activated with ozone resound on cellular redox balance, improves lipoatrophy and quality of life in HIV patients

[La bioestimulacion facial con PRP activado con ozono repercute en el balance redox, mejora la lipoatrofia y la calidad de vida de pacientes VIH]

Lizette Gil-del Valle1*, Miguel A. Acosta Suarez2, Carlos L. Rabeiro-Martinez1, Rosario Gravier-Hernández1, María C. Hernández González-Abreu1, Yusimit Bermúdez-Alfonso1, Mayda Rosa-Font2, Jorge Campos-Díaz2, Daymé Hernández-Requejo2, Gregorio Martínez-Sánchez3, Mark T. Weiser4

1Pharmacological Research Department, Institute “Pedro Kourí” (IPK), Autopista Novia del Mediodía km 6 ½, La Habana, Cuba.
 2Hospital, Institute “Pedro Kourí” (IPK), La Habana, Cuba.
3Freelance, Scientific advisor, Ancona, Italy.
4Ozonetherapie group, California, USA.

*E-mail: lgil@ipk.sld.cu

Abstract

Context: Pathogenic impact of high-grade local and systemic oxidative stress in antiretroviral treated HIV patients are recognized factor influencing in lipodystrophy, which is proposed can be ameliorate with platelet rich plasma (PRP).

Aims: To determine the efficacy and safety of ozone and calcium activated PRP application in lipoatrophy- AIDS Cuban individuals.

Methods: Thirty HIV individuals enrolled in quasi experimental prospective study showed lipoatrophy grade from 1 to 3. A mean volume interval of 8 to 22.2 mL of PRP was injected in 5 interventions during a year. The clinical, chemical, oxidative stress and progression indexes determinations were performed prior to injections and at 6 and 12 months after. Also, questionnaires based on Short Form 36, Medical Outcomes Study HIV Health Survey were assessed. Different statistical analyses were done comparing baseline respect final values of variables.

Results: Beneficial improve of lipoatrophy grade (p<0.05) and stabilization in global indexes of damage and antioxidant status at the end of the study was demonstrated. The comparison revealed a significantly smaller damage and higher antioxidant status compared to baseline values (p<0.05). Non-significant modifications were observed in hematological and chemical indexes (p˃0.05), respect quality of life 75% of three dimensions improved and depressive symptoms decreased. Non-adverse reactions were observed during the study period.

Conclusions: These results corroborate that beneficial amelioration of oxidative stress occurs in lipoatrophy AIDS patients during effective and safety PRP-ozone facial bioestimulation. Integral diagnosis would be worthwhile to conduct a more comprehensive study and manage of lipoatrophy.

Keywords: AIDS; HIV; lipoatrophy; oxidative stress; ozone; platelet-rich-plasma.

Resumen

Contexto: El impacto patogénico del estrés oxidativo local y sistémico en pacientes VIH tratados con antirretrovirales ha sido reconocido como un factor que influye en la lipoatrofia, la que puede reducirse con plasma rico en plaquetas (PRP).

Objetivos: Determinar la eficacia y la seguridad de la aplicación de PRP activado con ozono y calcio en la bioestimulación facial de la lipoatrofia de pacientes sida cubanos.

Métodos: Se realizó un estudio cuasi experimental prospectivo, con evaluación antes y después de realizar cinco aplicaciones de PRP con volúmenes de 8 a 22,2 mL durante un año en 30 pacientes VIH/Sida con grados de 1 a 3 de lipoatrofia. Se evaluaron indicadores hematológicos, hemoquímicos, de progresión y de estrés oxidativo. Las reacciones adversas y la calidad de vida de los pacientes fueron evaluadas según cuestionarios con modelos oficiales y el MOS 30 HIV, respectivamente. El análisis estadístico se realizó en correspondencia de las variables analizadas.

Resultados: La evaluación sistemática demostró la eficacia y la seguridad del procedimiento con una mejoría del grado de lipoatrofia (p<0,05) y estabilización de los índices de estrés oxidativo con aumento significativo del 75% de la calidad de vida en tres dimensiones (p<0,05). No se observaron modificaciones en las variables de laboratorio, ni reacciones adversas.

Conclusiones: El presente trabajo aporta evidencias sobre la estabilización del estrés oxidativo en pacientes sida cubanos con lipoatrofia tratados con PRP activado con ozono. El diagnostico integral se propone de utilidad para conducir estudios que mejoren la comprensión y el manejo de la lipoatrofia.

Palabras Clave: estrés oxidativo; lipoatrofia; ozono; plasma-rico-en-plaquetas; SIDA; VIH.

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Citation Format: Gil-del Valle L, Acosta Suarez MA, Rabeiro Martinez CL, Gravier-Hernández R, Hernández González-Abreu MC, Bermúdez-Alfonso Y, Rosa-Font M, Campos-Díaz J, Hernández-Requejo D, Martínez-Sánchez G, Weiser MT (2019) Facial biostimulation with PRP activated with ozone resound on cellular redox balance, improves lipoatrophy and quality of life in HIV patients. J Pharm Pharmacogn Res 7(4): 273–287.

© 2019 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

Irritabilidad dérmica y oftálmica de OLEOMASAJE

Dermatology and Cosmetic Science, Ozone, Preclinical Research, Toxicology
J Pharm Pharmacogn Res 3(4): 87-91, 2015.

Short Communication | Comunicación Corta

Evaluación de la irritabilidad dérmica y oftálmica de la formulación OLEOMASAJE

[Evaluation of the ophthalmic and dermal irritability of the OLEOMASAJE formulation]

Maritza F. Díaz1*, Kendra García2, Rosa I. Meneau3, Yaíma Sánchez3, Gastón García4

1Sociedad Médica Terapéutica LTDA, Martin de Zamora No. 3042, Las Condes, Santiago, Chile.2Centro Nacional de Investigaciones Científicas, 24 y 158, Cubanacán, Playa, La Habana, Cuba.3Centro para el Control Estatal de Medicamentos, 5ta A y 60, Playa, La Habana, Cuba.
4Centro de Estudios para las Investigaciones y Evaluaciones Biológicas, Instituto de Farmacia y Alimentos, 222 y 25, La Lisa, La Habana, Cuba.
Abstract

Context: Ozonized vegetable oils present germicidal effects which can be used as active principle in different cosmetological compositions. The thematic about of the corporal massage advantage have been very treatment by aesthetics, dermatologic and consumers. OLEOMASAJE formulation content ozonized sunflower oil OLEOZON as active principle active which present moisturizer and conditioner effects.

Aims: To determine the possible dermal and ophthalmic irritability effects of the OLEOMASAJE.

Methods: The trial was conducted in rabbits and the techniques described in the standardized procedures of the work established by the Center of Biological Research and Evaluations from Pharmacy and Food Institute of Havana University were used according to Norma ISO 10993-10. The ethical principles of the Good Practices of Laboratory were fulfilled to avoid suffering to the animals during the experimentation. Male rabbits with body weight between 2.05 and 2.48 kg were used. In the skin and the ocular structures the effects were observed immediately after of the application at 1, 24, 48 and 72 hours. Draize´s scale was applied to evaluate skin and ocular structures lesions.

Results: The OLEOMASAJE formulation does not irritate de skin of the assayed rabbits. However, the ocular structures irritability index was of 4.5 after of first hour to apply of product. These wounds were missing before of 24 hours to apply of product.

Conclusions: The OLEOMASAJE formulation obeys the indispensable requirements for the acceptance as cosmetic product and for it is used in corporal massage.

Keywords: Corporal massage; ozonized sunflower oil; topic OLEOZON.

Resumen

Contexto: Los aceites vegetales ozonizados presentan efectos germicidas los cuales pueden ser utilizados como principios activos en diferentes composiciones cosmetológicas. La temática acerca del beneficio del masaje corporal ha sido muy tratada por esteticistas, dermatólogos y consumidores. La formulación OLEOMASAJE contiene aceite de girasol ozonizado OLEOZON como principio activo y presenta efectos suavizante y humectante.

Objetivos: Determinar la posible irritabilidad dérmica y oftálmica del producto OLEOMASAJE.

Métodos: Los ensayos se llevaron a cabo en conejos con las técnicas descritas en los procedimientos normalizados de trabajo establecidos en el Centro de Investigaciones y Evaluaciones Biológicas de la Universidad de La Habana, según la Norma ISO 10993-10. Se cumplieron los principios de las Buenas Prácticas de Laboratorio y los principios éticos para evitar sufrimiento a los animales durante la experimentación. Se utilizaron conejos machos con peso corporal entre 2,05 y 2,48 kg. En la piel como en las estructuras oculares los efectos fueron observados inmediatamente después de la aplicación a las 1, 24, 48 y 72 horas. Se aplicó la escala Draize para evaluar las lesiones sobre la piel y las estructuras oculares.

Resultados: El producto OLEOMASAJE no resulta irritante en la piel de los conejos ensayados. Sin embargo en las estructuras oculares el índice de irritabilidad fue de 4,5 después de la primera hora de aplicación del producto. Estas lesiones desparecieron antes de las 24 horas de aplicado el producto.

Conclusiones: La formulación OLEOMASAJE cumple con los requisitos indispensables para ser aceptado como producto cosmético y ser utilizado como masaje corporal.

Palabras Clave: Aceite de girasol ozonizado; masaje corporal; OLEOZON tópico.

Download the PDF file .

Citation Format: Maritza F. Díaz, Kendra García, Rosa I. Meneau, Yaíma Sánchez, Gastón García (2015) Evaluación de la irritabilidad dérmica y oftálmica de la formulación OLEOMASAJE. [Evaluation of the ophthalmic and dermal irritability of the OLEOMASAJE formulation]. J Pharm Pharmacogn Res 3(4): 87-91.

© 2015 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

C 020: OZONE CONDITIONING EFFECT, NRF2 PATHWAY AND EPRE. AN IN VIVO STUDY DURING MAYOR AUTHOHEMOTHERAPY

Ozone
J Pharm Pharmacogn Res 2(Suppl. 1): S49, 2014

Special supplement with the abstract book of LATINFARMA 2013

Conference

C 020: OZONE CONDITIONING EFFECT, NRF2 PATHWAY AND EPRE. AN IN VIVO STUDY DURING MAYOR AUTHOHEMOTHERAPY

Re L,1, Martínez-Sánchez G2, Bordicchia M3, Malcangi G1, Pocognoli A3, Morales-Segura M4, Rothchild J5 and Rojas A.6

1Clinical Pharmacology & Toxicology Dept, Medinat SAS, Via Fazioli 22, 60021 Camerano, Ancona, Italy. Tel. +39 071 731076 Fax. +39 071 731347, E-Mail: lambertore@lambertore.com
2Medical Center Beauty Benefit – San Biagio di Osimo, Via Mons. Oscar Romero, 31 60027 Osimo, Ancona, Italy. Tel. +39 3483833064, E-Mail: gregorcuba@yahoo.it
3Department of Internal Medicine, University of Ancona, Politecnica delle Marche, 60131 Ancona, Italy.
4Dept. Pharmacology, Faculty of Medicine, University of Chile, Santiago de Chile, Chile.
5Holistic Dentist Clinic, 175 Mercado Street Suite115, Durango, CO 81301, USA.
6Biomedical Research Laboratories, Medicine Faculty, Catholic University of Maule, Talca, Chile.
Abstract

Despite the long experience in the field of medical ozone therapy, and its positive clinical effects when used at appropriate concentrations, the most intimate molecular mechanisms are still partially unknown. The aim of our ongoing study, of which the present represents only the first step, was to verify some hypotheses to study on humans the involvement of the EpRE (electrophile-responsive element) system. Six adult healthy subjects of both gender and Caucasian ethnicity were eligible to participate in the study. Mayor Auto-hemotherapy (MAH) was performed, using a dose of ozone of 45 μg/kg of BW with ozone concentrations of 35 μg/mL. Patients were treated with 3 MAHs, one every 2 days interval. Blood samples for biochemical analysis were obtained after a 12 h overnight fast, at: 1) Immediately before the first MAH, 2) From the bottle during the first MAH after bubbling blood with ozone, 3) From the patients 30 min after the first MAH, 4) From the patients the day after the application of the third MAH. Periphery lymphocytes were isolated to assay Nrf2 levels and erythrocytes lysate were obtained to measure oxidative stress mediators. Nrf2 binding activity was increased (p<0.01) in human lymphocytes directly exposed to O3 and also (p<0.005) in circulating lymphocytes after MAH. Nrf2 signal back to normal status during the time. The initial activation of Nrf2 induced by ozone increase the level of antioxidants enzymes. These data provide new evidences on the mechanism responsible for the induction of antioxidant enzymes expression by ozonated MAH, and have a practical and clinical importance as an approach to the treatment of patients targeted to the restoration of redox homeostasis.

C 019: OZONE´S THERAPEUTICAL TARGETS. BASIS OF THEIR PLEIOTROPIC EFFECTS

Ozone
J Pharm Pharmacogn Res 2(Suppl. 1): S47, 2014

Special supplement with the abstract book of LATINFARMA 2013

Conference

C 019: OZONE´S THERAPEUTICAL TARGETS. BASIS OF THEIR PLEIOTROPIC EFFECTS

León Fernández OS, Díaz Soto MT, Dranguet Vaillant J.

Pharmacy and Food Institute of the University of Havana, Cuba.
Abstract

Ozone´s therapeutic effects have been demonstrated in different diseases which are “seeminlgy” no connected between them. Ozone Oxidative Pre/Postconditioning mechanism showed that ozone administration in low doses and controlled number of treatments was able to increase antioxidant endogenous defence systems therefore a condition in order to expect a therapeutical success with ozonetherapy is that disease has a chronic oxidative stress as component of its etiology so cellular redox balance happen in a therapeutical target of medical ozone. Superoxide dismutase activity is an enzyme which is involved in different diseases, mainly vascular disorders. At the same time, adenosine plays an important role in stress conditions. Both, superoxide dismutase and adenosine, are ubiquitous molecules, achieve its effects through signal transductions and they are modified in specific way by ozonetherapy. Above mentioned ozone´s therapeutical targets are shown through diseases as ischemic syndrome, diabetes and disc hernia which demonstrate ozonetherapy pleiotropic effects.