Category Archives: Pimenta racemosa

Antimicrobial activity of Pimenta racemosa var. racemosa

antimicrobial activity, C. krusei, essential oil, MRSA, Pimenta racemosa
J Pharm Pharmacogn Res 4(6): 224-230, 2016.

Original Article | Artículo Original

Antimicrobial activity of essential oil of Pimenta racemosa var. racemosa (Myrtaceae) leaves

[Actividad antimicrobiana del aceite esencial de las hojas de Pimenta racemosa var. racemosa (Myrtaceae)]

Billmary Z. Contreras-Moreno1,2,3*, Judith J. Velasco4, Janne del C. Rojas2,5, Lucero del C. Méndez1,2,3, María T. Celis1

1Laboratory of Polymers and Colloids (POLYCOL), Faculty of Engineering, University of Los Andes (ULA), Ave. Don Tulio Febres Cordero, Mérida 5101, Venezuela.
2Laboratory “C” of Natural Products, Research Institute, Faculty of Pharmacy and Bioanalysis, University of Los Andes (ULA), Mérida, Venezuela.
3Natural Products Research Group (GIPRONA), Nucleus University Rafael Urdaneta (NURR), University of Los Andes (ULA), Trujillo, Venezuela.
4Microbiology and Parasitology Department, Faculty of Pharmacy and Bioanalysis, University of Los Andes (ULA), Mérida, Venezuela.
5Organic Biomolecular Research Group, Research Institute, Faculty of Pharmacy and Bioanalysis, University of Los Andes (ULA), Mérida, Venezuela.

*E-mail: billmary.contreras@gmail.com

Abstract

Context: Essential oils represent a therapeutic alternative in natural products against pathogenic bacteria that have become resistant to antibiotics and threaten public health and individual health of patients.

Aims: To determine the antimicrobial activity of two essential oils of different densities, obtained by hydrodistillation of Pimenta racemosa var. racemosa fresh leaves collected from Táchira, Venezuela against different multirresistant bacterial strains of nosocomial origin.

Methods: Disc diffusion agar method was carried out against seven reference strains: Candida albicans (CDC-B385), Candida krusei (ATCC 6258), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 23357), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923) and three different bacterial strains of nosocomial origin: Methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum β-lactamase (ESBL) producing Escherichia coli and Enterobacter cloacae.

Results: The essential oils of Pimenta racemosa var. racemosa inhibited the development of all microorganisms tested with minimum inhibitory concentration (MIC) values ranging from 20 to 400 μL/mL.

Conclusions: This is the first report concerning antimicrobial activity of essential oils obtained from Pimenta racemosa var. racemosa collected from Táchira, Venezuela with different densities. Furthermore, results showed the essential oils of this species might be an alternative as antimicrobial agent for the pharmaceutical industry.

Keywords: antimicrobial activity; C. krusei; essential oil; MRSA; Pimenta racemosa.

Resumen

Contexto: Los aceites esenciales representan una alternativa terapéutica en productos naturales contra bacterias patógenas que se han hecho resistentes a los antibióticos y que amenazan la salud pública y la salud individual de los pacientes.

Objetivos: Evaluar la actividad antimicrobiana de dos aceites esenciales de diferentes densidades, obtenidos por hidrodestilación de las hojas frescas de Pimenta racemosa var. racemosa recolectadas en Táchira, Venezuela frente a diferentes cepas bacterianas multiresistentes de origen nosocomial.

Métodos: Se usó el método de difusión en agar con disco para evaluar la actividad antimicrobiana de los aceites esenciales frente a siete cepas de referencia internacional: Candida albicans (CDC-B385), Candida krusei (ATCC 6258), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 23357), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923); y tres cepas bacterianas de origen nosocomial Staphylococcus aureus resistente a meticilina (SARM), Escherichia coli y Enterobacter cloacae productoras de β-lactamasa de espectro extenso (BLEE).

Resultados: Los aceites esenciales de Pimenta racemosa var. racemosa evaluados inhibieron el desarrollo de todos los microorganismos ensayados con valores de concentración inhibitoria mínima (CIM) que oscilaron entre 20 y 400 µL/mL.

Conclusiones: Este es el primer reporte sobre actividad antimicrobiana de los aceites de diferentes densidades de esta especie colectada en Táchira, Venezuela. Además, los resultados revelaron que los aceites esenciales de esta especie pueden ser una alternativa como agente antimicrobiano para la industria farmacéutica.

Palabras Clave: aceite esencial; actividad antimicrobiana; C. krusei; Pimenta racemosa; SARM.

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Citation Format: Contreras-Moreno BZ, Velasco JJ, Rojas JC, Méndez LC, Celis MT (2016) Antimicrobial activity of essential oil of Pimenta racemosa var. racemosa (Mill.) J.W. Moore (Myrtaceae) leaves. J Pharm Pharmacogn Res 4(6): 224-230.
This article has been cited by:
Contreras-Moreno BZ (2018) Chemical composition of essential oil of genus Pimenta (Myrtaceae): Review. In: Potential of Essential Oils, Hany A. El-Shemy, IntechOpen. DOI: http://dx.doi.org/10.5772/intechopen.78004
Azuaje M, Villarreal S, Rojas-Fermín L, Díaz C, Velasco J, Salazar O, Rodríguez M (2017) In vitro antifungal activity of extracts of Hura crepitans L. (Euphorbiaceae) against Candida albicans. Avances en Biomedicina 6(3): 197-202. Website
Jelena Bogojević, Miloš Nikolić, Tatjana Marković, Ana Ćirić, Dejan Marković (2016) Analysis of chemical composition of the most efficient essential oils towards Enterococcus faecalis referent strain ATCC 29212 and clinical isolates. Lek. Sirov. XXXVI(36):3–25. Website. DOI: 10.5937/leksir1636003B

© 2016 Journal of Pharmacy & Pharmacognosy Research (JPPRes)