J. Pharm. Pharmacogn. Res., vol. 11, no. 2, pp. 229-242, March-April 2023.

DOI: https://doi.org/10.56499/jppres22.1505_11.2.229

Original Article

Chitosan–alginate porous scaffold incorporated with hydroalcoholic Zingiber officinale Roscoe extract for neural tissue engineering

[Estructura porosa de quitosano-alginato incorporada con extracto hidroalcohólico de Zingiber officinale Roscoe para la ingeniería del tejido neural]

Hassan Sohrabian Kafraj¹, Maryam Alipour², Abdolhosein Shiravi¹, Vida Hojati¹, Mojtaba Khaksarian²*

¹Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.

²Razi Herbal Medicines Research Center and Department of Physiology, Lorestan University of Medical Sciences, Khorramabad, Iran.

*E-mail: mojkhaksar@lums.ac.ir, mojkhaksar@yahoo.com

Abstract

Context: Preparing a suitable substrate for the culture of neural stem cells and their proliferation in neural tissue engineering is of paramount importance.

Aims: To evaluate the effect of the hydroalcoholic Zingiber officinale extract incorporated in the chitosan-alginate scaffold (Chi-Alg-Zo) on nerve tissue.

Methods: The porous scaffolds developed in the present study were investigated in terms of their surface properties, chemical interaction, crystallinity, thermal stability, porosity percentage, pore sizes, degradability, and water absorption properties. To this end, the following tests were performed: Field emission scanning electron microscope (FE-SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), thermal gravimetric analysis (TGA), porosity based on liquid replacement, Image-J analysis, and water degradation and absorption test. Mouse neural stem/progenitor cells (NS/PCs) were harvested from the embryonic mouse brain. NSCs were seeded on scaffolds incorporated with hydroalcoholic Z. officinale extract. The MTT assay was done for the survival and the proliferation of neural stem/progenitor cells (NS/PCs) on scaffolds.

Results: Results indicated the good capacity of Chi-Alg-Zo for proliferation and differentiation into glial (astrocytes and oligodendrocytes) lineages. A suitable surface, which was provided for cellular interaction, led to the advancement of cell survival, connectivity, proliferation, and separation of NSCs.

Conclusions: The present study evaluated the separation of stem cells on the scaffold, finding that the expression of the glial fibrillary acidic protein (GFAP) and Oligo4 markers was higher in Chi-Alg scaffolds containing hydroalcoholic Z. officinale extract. Chi-Alg-Zo scaffolds could be suitable candidates for neural tissue engineering.

Keywords: neural tissue engineering; neural stem/progenitor cells; chitosan-alginate scaffold; ginger extract.

Resumen

Contexto: La preparación de un sustrato adecuado para el cultivo de las células madre neurales y su proliferación en la ingeniería del tejido neural es de suma importancia.

Objetivos: Evaluar el efecto del extracto hidroalcohólico de Zingiber officinale incorporado al andamio de quitosano-alginato (Chi-Alg-Zo) en el tejido nervioso.

Métodos: Los andamios porosos desarrollados en el presente estudio fueron investigados en cuanto a sus propiedades superficiales, interacción química, cristalinidad, estabilidad térmica, porcentaje de porosidad, tamaño de los poros, degradabilidad y propiedades de absorción de agua. Para ello, se realizaron las siguientes pruebas: Microscopio electrónico de barrido de emisión de campo (FE-SEM), espectroscopia infrarroja por transformada de Fourier (FTIR), difracción de polvo de rayos X (XRD), análisis termo gravimétrico (TGA), porosidad basada en la sustitución de líquidos, análisis Image-J, y ensayo de degradación y absorción de agua. Las células madre/progenitoras neurales de ratón (NSC/PCs) fueron cosechadas del cerebro embrionario de ratón. Las NSC se sembraron en andamios incorporados con extracto hidroalcohólico de Z. officinale. Se realizó el ensayo MTT para la supervivencia y la proliferación de las células madre/progenitoras neurales (NS/PCs) en los andamios.

Resultados: Los resultados indicaron la buena capacidad de Chi-Alg-Zo para la proliferación y diferenciación en linajes gliales (astrocitos y oligodendrocitos). Una superficie adecuada, que se proporcionó para la interacción celular condujo al avance de la supervivencia celular, la conectividad, la proliferación y la separación de las NSC.

Conclusiones: El presente estudio evaluó la separación de las células madre en el andamio, encontrando que la expresión de los marcadores de proteína ácida fibrilar glial (GFAP) y Oligo4 era mayor en los andamios Chi-Alg que contenían extracto hidroalcohólico de Z. officinale. Los andamios Chi-Alg-Zo podrían ser candidatos adecuados para la ingeniería del tejido neural.

Palabras Clave: ingeniería de tejidos neurales; células madre/progenitoras neurales; andamio de quitosano-alginato; extracto de jengibre.

Citation Format: Sohrabian Kafraj H, Alipour M, Shiravi A, Hojati V, Khaksarian M (2022) Chitosan-alginate porous scaffold incorporated with hydroalcoholic Zingiber officinale Roscoe extract for neural tissue engineering. J Pharm Pharmacogn Res 11(2): 229–242. https://doi.org/10.56499/jppres22.1505_11.2.229

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