J. Pharm. Pharmacogn. Res., vol. 12, suppl. 1, pp. S69-S74, Dec 2024.
DOI: https://doi.org/10.56499/jppres23.1769_12.s1.69
Short Communication
Effect of chitosan nanoparticles from red snapper scales (Lutjanus sp.) on pulp repair: In vivo study
Noor Hafida Widyastuti1*, Adi Prayitno2, Risya Cilmiaty2, Brian Wasita3
1Department of Conservative Dentistry, Faculty of Dentistry, Universitas Muhammadiyah Surakarta, Indonesia.
2Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia.
3Department of Histology, Faculty of Medicine, Sebelas Maret University, Surakarta, Indonesia.
*E-mail address: noor.hafida@student.uns.ac.id; ORCID: 0000-0001-7210-1319
Abstract
Context: Iatrogenic actions leading to exposed pulp can result in pulp inflammation, with Reversible Pulpitis being a common condition among the general population. Pulp capping treatments are essential for supporting pulp repair and promoting the formation of reparative dentin. The choice of pulp capping material plays a crucial role in the success of this treatment.
Aims: To assess the impact of chitosan nanoparticles (CN) derived from red snapper scales (Lutjanus sp.) on pulp repair, as indicated by the expression of TGF-β1 and the formation of reparative dentin.
Methods: The research began with the preparation of CN extracted from red snapper fish scales using the ionic gelation method. Subsequently, an experimental animal model of reversible pulpitis was established. The study was conducted in vivo using 18 Sprague Dawley rats. Three groups were included: reversible pulpitis, reversible pulpitis with Ca(OH)2 application, and reversible pulpitis with CN application. Observations were made regarding the expression of TGF-β using ELISA and the formation of reparative dentin through histopathological examination.
Results: The results indicated a significant difference between groups, with a one-way ANOVA Test yielding a p-value of 0.049 for TGF-β1 expression and a Kruskal-Wallis test yielding a p-value of 0.009 for dentin reparative formation.
Conclusion: Chitosan nanoparticles derived from red snapper scales have demonstrated the ability to enhance pulp repair by elevating TGF-β expression and promoting the formation of reparative dentin.
Keywords: chitosan nanoparticle; pulp repair; TGF-β1; reparative dentin.
Paper presented at the 4th International Conference on Medicine and Health Sciences (ICO-Helics), 2023.
Citation Format: Widyastuti NH, Prayitno A, Cilmiaty R, Wasita B (2024) Effect of chitosan nanoparticles from red snapper scales (Lutjanus sp.) on pulp repair: In vivo study. J Pharm Pharmacogn Res 12(suppl. 1): S69–S74. https://doi.org/10.56499/jppres23.1769_12.s1.69
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