CO 023: TRIMETAZIDINE INHIBITS THE RENAL FIBROSIS INDUCED BY CYCLOSPORIN A

Excerpt:


J Pharm Pharmacogn Res 2(Suppl. 1): S12, 2014 Special supplement with the abstract book of LATINFARMA 2013 Oral Communication CO 023: TRIMETAZIDINE INHIBITS THE RENAL FIBROSIS INDUCED BY CYCLOSPORIN A De la Cruz Rodríguez L, Rey M, Honoré S, Oldano A, Araujo C. Facultad de Bioquímica Química y Farmacia. Universidad Nacional de Tucumán, Balcarce 747. … Continue reading CO 023: TRIMETAZIDINE INHIBITS THE RENAL FIBROSIS INDUCED BY CYCLOSPORIN A

J Pharm Pharmacogn Res 2(Suppl. 1): S12, 2014

Special supplement with the abstract book of LATINFARMA 2013

Oral Communication

CO 023: TRIMETAZIDINE INHIBITS THE RENAL FIBROSIS INDUCED BY CYCLOSPORIN A

De la Cruz Rodríguez L, Rey M, Honoré S, Oldano A, Araujo C.

Facultad de Bioquímica Química y Farmacia. Universidad Nacional de Tucumán, Balcarce 747. (4000) San Miguel de Tucumán, Argentina. E-mail: cdantur@fbqf.unt.edu.ar
Abstract

Introduction: Cyclosporin A (CyA) is an immunosuppressor used in transplanted patients. Adverse effects like as nephrotoxicity and hepatotoxicity, have been described. Our previous papers demonstrated that Trimetazidine (TMZ) is able to reverse the CyAinduced nephrotoxicity.

Objectives: contribute to the knowledge of the mechanisms of action of TMZ in renal fibrosis induced by CyA.

Material and methods: The experimental design was carried out for 120 days. Included four groups (n = 8) of male Wistar rats treated with: A control. B treated with CyA (25 mg/kg/day). C treated with TMZ (20 mg/kg/day). D treated TMZ for 20 days (20 mg/kg/day), them for 120 days with TMZ (20 mg/kg/day) and CyA (25 mg/kg/day). Urea and serum creatinine as well as the excretion of urinary gamma glutamyl transpeptidase were determined. Structural studies were performed with hematoxylin-eosin and Mallory staining to evidence the renal fibrosis. Fibrosis was quantified using the Image Pro Plus software (NIH). By Transmission electron microscope (TEM) the renal ultrastructure was analyzed. Transforming Growth Factor beta and Collagen I were evidenced by immunohistochemistry.

Results: animals treated with 25 mg CyA/kg/day presented hydropic degeneration with nuclei displaced and in some cases are found in the tubular lumen, observed by optical microscopy. Ultrastructure analyzed by TEM showed edematous mitochondria with loss of its internal structure. Interstitial fibrosis was confirmed by evidenced immunohistochemistry Transforming Growth Factor Beta and Collagen I using specific antibodies. Pre-treatment with TMZ for 20 days and the synergistic treatment TMZ+CyA, showed biochemical parameters within the reference values and preserved cytoarchitecture, with negative immunohistochemistry to the markers studied.

Conclusions: Interstitial fibrosis induced by CyA was dose and time dependent. The production of inflammatory mediators, Transforming Growth Factor Beta and the synthesis of Collagen I, would be inhibited by TMZ.

Alendronate and platelet-rich plasma in sheep anterior cruciate ligament
J. Pharm. Pharmacogn. Res., vol. 12, no. 3, pp. 526-538, May-Jun 2024. DOI: https://doi.org/10.56499/jppres23.1845_12.3.526 Original Article Effect of alendronate and platelet-rich plasma in tendon-bones integration in sheep anterior cruciate ligament surgery [Efecto del alendronato y del plasma rico en plaquetas en la integración tendón-hueso en la cirugía del ligamento cruzado anterior ovino] Tangkas Sibarani1*, Bambang … Continue reading Alendronate and platelet-rich plasma in sheep anterior cruciate ligament
Brassicaceae selenium complexes versus SARS-COV-2 MAP4 and spike protein
J. Pharm. Pharmacogn. Res., vol. 12, no. 3, pp. 514-525, May-Jun 2024. DOI: https://doi.org/10.56499/jppres23.1739_12.3.514 Original Article Antiviral potential of selenium complexes from Brassicaceae by inhibiting protein bond between MAP4 and the spike of SARS-CoV-2 [Potencial antiviral de complejos de selenio de Brassicaceae mediante la inhibición de la unión proteica entre MAP4 y la espiga de … Continue reading Brassicaceae selenium complexes versus SARS-COV-2 MAP4 and spike protein
Andrographis paniculata and cardiovascular diseases
J. Pharm. Pharmacogn. Res., vol. 12, no. 3, pp. 487-513, May-Jun 2024. DOI: https://doi.org/10.56499/jppres23.1841_12.3.487 Review Andrographis paniculata: A potential supplementary therapy for cardiovascular diseases - A systematic review of its effects and molecular actions [Andrographis paniculata: Una terapia suplementaria potencial para las enfermedades cardiovasculares - Una revisión sistemática de sus efectos y acciones moleculares] Oluebube … Continue reading Andrographis paniculata and cardiovascular diseases

© 2013-2020 by the authors; licensee JPPRes, Antofagasta, Chile. This journal is an open-access journal distributed under the terms and conditions of the Creative Commons Attribution license-Non Commercial 4.0 international. The content on this site is intended for health professionals. If you are not a health professional, please talk to your doctor about any doubts or concerns regarding your health

Made with ♥ by AVAGAX Studio