J Pharm Pharmacogn Res 2(Suppl. 1): S43, 2014
Special supplement with the abstract book of LATINFARMA 2013
CO 070: IMMUNOMODULATING AND ENHANCING ACTIVITIES OF SOLUBLE GLUCANS FROM Sacharomyces cerevisiae ASSESED BY MONOCYTE ACTIVATION TEST
Pardo-Ruiz Z, Perdomo-Morales R, Pacios-Michelena A.
Introduction: Beta-glucans are homopolymers of glucose that have shown immunomodulating effects depending on their structural characteristics and could be potential contaminants of some parenterals that are clarified through cellulose filters. The ability of glucans to develop an immune response or enhance the immune response induced by other pathogens is overlooked by the conventional pyrogen tests. Thus, the objective of this work was to determine the enhancing immune effect of glucans from the yeast Sacharomyses cerevisiae when they are incubated concomitantly with LPS and to confirm the significance of Monocyte Activation Test (MAT) for detecting such activities.
Material and methods: Soluble glucans were purified from S. cerevisiae and were partially characterized. The content of hexoses was determined by phenol-sulphuric colorimetric method and endotoxin contamination was evaluated by the Limulus test. Both immunostimulating and enhancing effects were determined by employing MAT. As monocyte source was used human whole blood that was incubated in polypropilene tubes with glucans, either alone or concomitantly with LPS. The releasing of Interleukin-6, -1β and Tumor Necrosis Factor-α was determined in the supernatant by ELISA.
Results: Glucans induced the releasing of all proinflammatory cytokines assayed, TNF-α was the most released cytokine, followed by IL-6 and IL-1β. In addition, the LPS induced-immunostimulating effect was powerfully enhanced by glucans.
Conclusions: Glucans showed enhancing and immunomodulating effects that became evident through MAT. It was shown that MAT could be an appropriate test to evaluate glucans activities.