Effect of Centella asiatica in zebrafish insomnia model

Excerpt:


J. Pharm. Pharmacogn. Res., vol. 12, no. 2, pp. 286-295, Mar-Apr 2024. DOI: https://doi.org/10.56499/jppres23.1737_12.2.286 Original Article Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia model [Efecto de los extractos de Centella asiatica (L.) Urb. a través de la expresión de SIRT1 y Per2 en … Continue reading Effect of Centella asiatica in zebrafish insomnia model

J. Pharm. Pharmacogn. Res., vol. 12, no. 2, pp. 286-295, Mar-Apr 2024.

DOI: https://doi.org/10.56499/jppres23.1737_12.2.286

Original Article

Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia model

[Efecto de los extractos de Centella asiatica (L.) Urb. a través de la expresión de SIRT1 y Per2 en el modelo de insomnio de larvas de pez cebra (Danio rerio)]

Zamroni Afif1,2, Nurvia Andriani2, Rodhiyan Rakhmatiar2, Shahdevi Kurniawan2, Holipah3, Husnul Khotimah4*, Nurdiana4, Irawan Satriotomo5

1Doctoral Program in Medical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.

2Department of Neurology Faculty of Medicine, Universitas Brawijaya, Saiful Anwar General Hospital, Malang, Indonesia.

3Department of Public Health, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.

4Departement of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.

5Indonesian Neuroscience Institute, Malang, Indonesia.

*E-mail: husnul_farmako.fk@ub.ac.id

Abstract

Context: Insomnia is difficulty falling asleep or maintaining sleep or sleeping conditions that cannot adequately restore the body and mental state. However, insomnia therapy has not been satisfying for improving the condition. Several alternative therapy options for treating insomnia include Centella asiatica (CA). Many pathways lead to sleep regulation, including circadian rhythms and cellular reactions. Further research is necessary to treat insomnia through circadian rhythms by administering CA with observations on zebrafish models of insomnia.

Aims: To evaluate the influence of CA extract administration on deacetylase sirtuin-1 (SIRT1) and period circadian regulator-2 (Per2) expression in zebrafish (Danio rerio) larvae in an insomnia model.

Methods: A laboratory experimental study with a randomized control group post-test only was used, with a sample of D. rerio larvae insomnia model that was given treatment with CA ethanolic extract (2.5, 5, and 10 µg/mL). The D. rerio larvae were prepared for reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to evaluate SIRT1 and Per2 levels.

Results: This study found a significant effect of CA ethanolic extract on the expression of SIRT1 (p=0.001), with CA extract 10 µg/mL elevating SIRT1 expression in the group of D. rerio larvae with the insomnia model. There was no significant effect of CA extract on Per2 expression in the group of D. rerio larvae with the insomnia model (p=0.051).

Conclusions: Administration of CA extract at a concentration of 10 µg/mL can elevate the expression of SIRT1 and have no significant effect on Per2 expression in D. rerio larvae with insomnia model.

Keywords: Centella asiatica; circadian rhythm; deacetylase sirtuin-1; insomnia; period circadian regulator-2; zebrafish.

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Resumen

Contexto: El insomnio es la dificultad para conciliar o mantener el sueño o las condiciones de sueño que no pueden restaurar adecuadamente el cuerpo y el estado mental. Sin embargo, la terapia del insomnio no ha sido satisfactoria para mejorar la condición. Varias opciones de terapia alternativa para tratar el insomnio incluyen Centella asiatica (CA). Muchas vías conducen a la regulación del sueño, incluidos los ritmos circadianos y las reacciones celulares. Es necesario seguir investigando para tratar el insomnio a través de los ritmos circadianos mediante la administración de CA con observaciones en modelos de insomnio con pez cebra.

Objetivos: Evaluar la influencia de la administración de extracto de CA en la expresión de la deacetilasa sirtuina-1 (SIRT1) y del regulador circadiano del periodo-2 (Per2) en larvas de pez cebra (Danio rerio) en un modelo de insomnio.

Métodos: Se utilizó un estudio experimental de laboratorio con un grupo de control aleatorizado post-test únicamente, con una muestra de larvas de D. rerio modelo de insomnio a las que se administró tratamiento con extracto etanólico de CA (2,5; 5 y 10 µg/mL). Las larvas de D. rerio se prepararon para la reacción en cadena de la polimerasa de transcripción inversa-cuantitativa (RT-qPCR) para evaluar los niveles de SIRT1 y Per2.

Resultados: Este estudio encontró un efecto significativo del extracto etanólico de CA sobre la expresión de SIRT1 (p=0,001), con el extracto de CA 10 µg/mL elevando la expresión de SIRT1 en el grupo de larvas de D. rerio con el modelo de insomnio. No hubo un efecto significativo del extracto de CA sobre la expresión de Per2 en el grupo de larvas de D. rerio con el modelo de insomnio (p=0,051).

Conclusiones: La administración de extracto de CA a una concentración de 10 µg/mL puede elevar la expresión de SIRT1 y no tener un efecto significativo sobre la expresión de Per2 en larvas de D. rerio con modelo de insomnio.

Palabras Clave: Centella asiática; deacetilasa sirtuin-1; insomnio; pez cebra; regulador circadiano del periodo-2; ritmo circadian.

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Citation Format: Afif Z, Andriani N, Rakhmatiar R, Holipah, Kurniawan S, Khotimah H, Nurdiana, Satriotomo I (2024) Effect of Centella asiatica (L.) Urb. extracts through expression of SIRT1 and Per2 on zebrafish (Danio rerio) larvae insomnia model. J Pharm Pharmacogn Res 12(2): 286–295. https://doi.org/10.56499/jppres23.1737_12.2.286
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