Effects of diet and exercise on mice cardiomyocytes

Excerpt:


J. Pharm. Pharmacogn. Res., vol. 12, no. 6, pp. 1121-1128, Nov-Dec 2024. DOI: https://doi.org/10.56499/jppres24.2015_12.6.1121 Original Article Effects of intermittent restriction diet and moderate-intensity exercise on the expression of cardiomyocytes in mice with high-glucose loads [Efecto de la dieta de restricción intermitente y del ejercicio de intensidad moderada en la expresión de los cardiomiocitos de ratones … Continue reading Effects of diet and exercise on mice cardiomyocytes

J. Pharm. Pharmacogn. Res., vol. 12, no. 6, pp. 1121-1128, Nov-Dec 2024.

DOI: https://doi.org/10.56499/jppres24.2015_12.6.1121

Original Article

Effects of intermittent restriction diet and moderate-intensity exercise on the expression of cardiomyocytes in mice with high-glucose loads

[Efecto de la dieta de restricción intermitente y del ejercicio de intensidad moderada en la expresión de los cardiomiocitos de ratones con cargas elevadas de glucosa]

Eka Arum Cahyaning Putri1,2*, Gosy Endra Vigriawan3, Hayuris Kinandita Setiawan2, Zulhabri Othman4, Yudi Her Oktaviano5, Lilik Herawati1,2

1Sport Health Science Magister Program, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.

2Department of Medical Physiology and Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.

3Doctoral Program, Faculty of Sports and Health, Universitas Negeri Surabaya, Indonesia.

4Postgraduate Centre, Management and Science University, Shah Alam, Selangor, Malaysia.

5Department of Cardiology and Vascular Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.

*E-mail: eka-arum-cp@fk.unair.ac.id

Abstract

Context: The prevalence of obesity was 8% in 2002 and increased to 11.5% in 2011 in Indonesia. The results of the 2018 Basic Health Research stated that 13.6% of people over the age of 18 were overweight, and 21.8% were obese.

Aims: To analyze the effect of intermittent restriction diet and continuous exercise with moderate intensity on individuals exposed to a high-calorie diet.

Methods: A randomized posttest control group design was used as the research design, with 6 mice in each group as the study subjects. The groups in this study were the control group with a high-calorie diet (CON+), the intermittent restriction diet group (IRD), the moderate-intensity continuous exercise group (MEX), the combined intermittent restriction diet group and the moderate-intensity continuous exercise (HYB) group. The measured variables were cardiomyocyte diameter and brain natriuretic peptide (BNP) expression.

Results: The results of this study were cardiomyocyte diameter CON+ (27.94 ± 5.65 µm), IRD (20.99 ± 11.80 µm), MEX (25.08 ± 9.14 µm), HYB (24.52 ± 5.90 µm) with p=0.578. Expression scores of BNP CON+ (6.00 ± 1.50), IRD (4.67 ± 1.00), MEX (5.42±2.09), HYB (6.27 ± 1.54) with p=0.335. These results showed no significant difference, but the intermittent restriction diet showed the most optimal mean with the lowest mean cardiomyocyte diameter and BNP expression.

Conclusions: There was no effect between the combination of an intermittent restriction diet and moderate-intensity continuous exercise on cardiomyocyte diameter and BNP expression. However, there is a potential that an intermittent restriction diet has the optimal effect in preventing changes in the cardiac structure.

Keywords: cardiomyocyte; diet; exercise; glucose; healthy lifestyle.

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Resumen

Contexto: La prevalencia de la obesidad era del 8% en 2002 y aumentó al 11,5% en 2011 en Indonesia. Los resultados de la Investigación Básica de Salud de 2018 afirmaron que el 13,6% de las personas mayores de 18 años tenían sobrepeso y el 21,8% eran obesas.

Objetivos: Analizar el efecto de la dieta de restricción intermitente y el ejercicio continuo con intensidad moderada en individuos expuestos a una dieta alta en calorías.

Métodos: Como diseño de investigación se utilizó un diseño de grupo control postest aleatorizado, con 6 ratones en cada grupo como sujetos de estudio. Los grupos de este estudio fueron el grupo de control con dieta hipercalórica (CON+), el grupo de dieta de restricción intermitente (IRD), el grupo de ejercicio continuo de intensidad moderada (MEX), el grupo de dieta de restricción intermitente combinada y el grupo de ejercicio continuo de intensidad moderada (HYB). Las variables medidas fueron el diámetro de los cardiomiocitos y la expresión del péptido natriurético cerebral (BNP).

Resultados: Los resultados de este estudio fueron diámetro de cardiomiocitos CON+ (27,94 ± 5,65 µm), IRD (20,99 ± 11,80 µm), MEX (25,08 ± 9,14 µm), HYB (24,52 ± 5,90 µm) con p=0,578. Puntuaciones de expresión de BNP CON+ (6,00 ± 1,50), IRD (4,67 ± 1,00), MEX (5,42±2,09), HYB (6,27 ± 1,54) con p=0,335. Estos resultados no mostraron diferencias significativas, pero la dieta de restricción intermitente mostró la media óptima con el diámetro cardiomiocítico medio y la expresión de BNP más bajos.

Conclusiones: No hubo efecto entre la combinación de una dieta de restricción intermitente y el ejercicio continuo de intensidad moderada sobre el diámetro de los cardiomiocitos y la expresión de BNP. Sin embargo, existe la posibilidad de que una dieta de restricción intermitente tenga el efecto más óptimo en la prevención de cambios en la estructura cardiaca.

Palabras Clave: acardiomiocitos; dieta; ejercicio; glucosa; estilo de vida saludable.

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Citation Format: Putri EAC, Vigriawan GE, Setiawan HK, Othman Z, Oktaviano YH, Herawati L (2024) Effects of intermittent restriction diet and moderate-intensity exercise on the expression of cardiomyocytes in mice with high-glucose loads. J Pharm Pharmacogn Res 12(6): 1121–1128. https://doi.org/10.56499/jppres24.2015_12.6.1121
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