Hepatoprotective potential of Vitis gracilis

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J. Pharm. Pharmacogn. Res., vol. 12, no. 6, pp. 1178-1186, Nov-Dec 2024. DOI: https://doi.org/10.56499/jppres24.1983_12.6.1178 Original Article Assessing the hepatoprotective efficacy of Vitis gracilis Wall. against doxorubicin-induced hepatic injury in rats [Evaluación de la eficacia hepatoprotectora de Vitis gracilis Wall. contra la lesión hepática inducida por doxorrubicina en ratas] Yurnadi Hanafi Midoen1, Syafruddin Ilyas2*, Putra Santoso3 … Continue reading Hepatoprotective potential of Vitis gracilis

J. Pharm. Pharmacogn. Res., vol. 12, no. 6, pp. 1178-1186, Nov-Dec 2024.

DOI: https://doi.org/10.56499/jppres24.1983_12.6.1178

Original Article

Assessing the hepatoprotective efficacy of Vitis gracilis Wall. against doxorubicin-induced hepatic injury in rats

[Evaluación de la eficacia hepatoprotectora de Vitis gracilis Wall. contra la lesión hepática inducida por doxorrubicina en ratas]

Yurnadi Hanafi Midoen1, Syafruddin Ilyas2*, Putra Santoso3

1Department of Medical Biology, Faculty of Medine, Universitas Indonesia, Jakarta, Indonesia.

2Study Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia.

3Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Andalas, Padang, Indonesia.

*E-mail: syafruddin6@usu.ac.id

Abstract

Context: Doxorubicin is an anticancer drug that can adversely affect the liver. Therefore, seeking medical attention to manage pain and minimize side effects is crucial.

Aims: To evaluate the hepatoprotective potential of Vitis gracilis Wall. nanoherbs against doxorubicin-induced hepatotoxicity.

Methods: The study included five treatment groups: T0 (negative control), T+ (positive control with doxorubicin alone), TC (doxorubicin + vitamin C at 0.2 mg/kg body weight/day), T125 (doxorubicin + V. gracilis at 125 mg/kg body weight/day), and T150 (doxorubicin + V. gracilis at 150 mg/kg body weight/day). Doxorubicin, administered intraperitoneally at 0.0019 mg/kg body weight once a week for three weeks, induced hepatotoxicity in the T+ group.

Results: Serum transaminases were significantly reduced in the T125 and T150 groups, indicative of the hepatoprotective effects of V. gracilis. Immunohistochemical analysis demonstrated the modulation of key markers, with decreased levels of pro-inflammatory tumor necrosis factor-α and increased levels of anti-inflammatory interleukin 10 in the T125 and T150 groups. The activities of oxidative stress markers, including superoxide dismutase and caspase 3, were favorably influenced by V. gracilis nanoherb treatment.

Conclusions: These findings suggest that V. gracilis nanoherbs may effectively attenuate doxorubicin-induced hepatotoxicity, evidenced by biochemical and immunohistochemical changes. This study provides a foundation for further exploration of V. gracilis nanoherbs as a potential adjunctive therapy in preventing chemotherapy-associated liver damage.

Keywords: CASP3; IL-10; serum transaminases; SOD; TNF-α.

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Resumen

Contexto: La doxorrubicina es un fármaco anticancerígeno que puede afectar negativamente al hígado. Por lo tanto, es fundamental buscar atención médica para controlar el dolor y minimizar los efectos secundarios.

Objetivos: Evaluar el potencial hepatoprotector de Vitis gracilis Wall. nanohierbas contra la hepatotoxicidad inducida por doxorrubicina.

Métodos: El estudio incluyó cinco grupos de tratamiento: T0 (control negativo), T+ (control positivo con doxorrubicina sola), TC (doxorrubicina + vitamina C a 0,2 mg/kg de peso corporal/día), T125 (doxorrubicina + V. gracilis a 125 mg/kg de peso corporal/día) y T150 (doxorrubicina + V. gracilis a 150 mg/kg de peso corporal/día). La doxorrubicina, administrada por vía intraperitoneal a 0,0019 mg/kg de peso corporal una vez por semana durante tres semanas, indujo hepatotoxicidad en el grupo T+.

Resultados: Las transaminasas séricas se redujeron significativamente en los grupos T125 y T150, lo que indica los efectos hepatoprotectores de V. gracilis. El análisis inmunohistoquímico demostró la modulación de marcadores clave, con niveles reducidos de factor de necrosis tumoral α proinflamatorio y niveles elevados de interleucina 10 antiinflamatoria en los grupos T125 y T150. Las actividades de los marcadores de estrés oxidativo, incluidas la superóxido dismutasa y la caspasa 3, se vieron influenciadas favorablemente por el tratamiento con nanoherbas de V. gracilis.

Conclusiones: Estos hallazgos sugieren que las nanohierbas de V. gracilis pueden atenuar eficazmente la hepatotoxicidad inducida por doxorrubicina, evidenciada por cambios bioquímicos e inmunohistoquímicos. Este estudio proporciona una base para una mayor exploración de las nanohierbas de V. gracilis como posible terapia complementaria para prevenir el daño hepático asociado a la quimioterapia.

Palabras Clave: CASP3; IL-10; SOD; TNF-α; transaminasas séricas.

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Citation Format: Midoen YH, Ilyas S, Santoso P (2024) Assessing the hepatoprotective efficacy of Vitis gracilis Wall. against doxorubicin-induced hepatic injury in rats. J Pharm Pharmacogn Res 12(6): 1178–1186. https://doi.org/10.56499/jppres24.1983_12.6.1178
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