Hibiscus surattensis: Insulin secretion and pancreatic tissue improvement

Excerpt:


J Pharm Pharmacogn Res 9(4): 454-464, 2021. Original article Insulin secretion and repairing pancreatic tissue damage on diabetic mice treated with the extract and active fraction of Hibiscus surattensis L. leaves [Secreción de insulina y reparación del daño del tejido pancreático en ratones diabéticos tratados con el extracto y la fracción activa de hojas de Hibiscus surattensis … Continue reading Hibiscus surattensis: Insulin secretion and pancreatic tissue improvement



J Pharm Pharmacogn Res 9(4): 454-464, 2021.

Original article

Insulin secretion and repairing pancreatic tissue damage on diabetic mice treated with the extract and active fraction of Hibiscus surattensis L. leaves

[Secreción de insulina y reparación del daño del tejido pancreático en ratones diabéticos tratados con el extracto y la fracción activa de hojas de Hibiscus surattensis L.]

Yuliet1*, Elin Yulinah Sukandar2, Nur Atik3, I Ketut Adnyana2

1Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu 94148, Central Sulawesi, Indonesia.

2Department of Pharmacology and Toxicology, School of Pharmacy, Bandung Institute of Technology, Bandung 40132, West Java, Indonesia.

3Department of Biomedical Sciences, Faculty of Medicine, Padjadjaran University, Jatinangor 45363, West Java, Indonesia.

*E-mail: yuliet@untad.ac.id; yuliet_susanto@yahoo.com

Abstract

Context: Diabetes is a chronic health condition with insulin disturbance. Hibiscus surattensis L. is a medicinal plant and plays a pivotal role in diabetes.

Aims: To evaluate the effect of H. surattensis leaves extract to increase insulin secretion and repair pancreatic tissue damage in streptozotocin (STZ)‑induced diabetic mice.

Methods: This research used male mice (Swiss Webster) administrated by STZ 100 mg/kg BW to induce diabetes. Animals were divided into eight groups (n = 6) consisting of normal control, diabetic control, glibenclamide, ethanol extract 50, ethyl acetate fraction, and water fraction with doses of 25 and 50 mg/kg BW, respectively. Treatment was given for 28 days. The evaluation was carried out on blood glucose levels, insulin levels, HOMA-β, and the pancreatic islets by the morphometric analysis were evaluated. All data were analyzed using SPSS 16.0.

Results: Extract and active fraction of H. surattensis leaves, especially ethyl acetate fractions (25 and 50 mg/kg BW) significantly (p<0.05) reduced fasting blood glucose levels, insulin levels, and the strength of pancreatic β cells (HOMA-β) increased in STZ induced diabetic mice. Histopathological results in pancreatic confirmed the effect of H. surattensis to increase the number of islets significantly in Langerhans and pancreatic β-cells compared to the diabetic control group (p<0.05).

Conclusions: Extract, and active fraction of H. surattensis leaves affect the insulin secretion and repairing the pancreatic tissue damage on diabetic mice, the most optimal therapy on the administration of ethyl acetate fraction.

Keywords: antidiabetic; Hibiscus surattensis L.; insulin; pancreas; streptozotocin.

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Resumen

Contexto: La diabetes es una enfermedad crónica con alteración de la insulina. Hibiscus surattensis L. es una planta medicinal y juega un papel fundamental en la diabetes.

Objetivos: Evaluar el efecto del extracto de hojas de H. surattensis para aumentar la secreción de insulina y reparar el daño del tejido pancreático en ratones diabéticos inducidos por estreptozotocina (STZ).

Métodos: Esta investigación utilizó ratones macho (Swiss Webster) administrados por STZ 100 mg/kg BW para inducir diabetes. Los animales se dividieron en ocho grupos (n = 6) que consistían en control normal, control diabético, glibenclamida, extracto de etanol 50, fracción de acetato de etilo y fracción de agua con dosis de 25 y 50 mg/kg de peso corporal, respectivamente. El tratamiento se administró durante 28 días. La evaluación se realizó sobre niveles de glucosa en sangre, niveles de insulina, HOMA-β, y se evaluaron los islotes pancreáticos mediante análisis morfométrico. Todos los datos se analizaron con SPSS 16.0.

Resultados: El extracto y la fracción activa de hojas de H. surattensis, especialmente las fracciones de acetato de etilo (25 y 50 mg/kg de peso corporal) redujeron significativamente (p<0,05) los niveles de glucosa en sangre en ayunas, los niveles de insulina y la fuerza de las células β pancreáticas (HOMA-β) aumentó en ratones diabéticos inducidos por STZ. Los resultados histopatológicos en páncreas confirmaron el efecto de H. surattensis para aumentar significativamente el número de islotes en Langerhans y células β pancreáticas en comparación con el grupo de control diabético (p<0,05).

Conclusiones: El extracto y fracción activa de hojas de H. surattensis afectan la secreción de insulina y reparan el daño del tejido pancreático en ratones diabéticos, la terapia más óptima sobre la administración de fracción de acetato de etilo.

Palabras Clave: antidiabético; estreptozotocina; Hibiscus surattensis L.; insulina; páncreas.

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Citation Format: Yuliet, Sukandar EY, Atik N, Adnyana IK (2021) Insulin secretion and repairing pancreatic tissue damage on diabetic mice treated with the extract and active fraction of Hibiscus surattensis L. leaves. J Pharm Pharmacogn Res 9(4): 454–464.

© 2021 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

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