IgM antibody and fungal load impacts of P. scutellarioides

Excerpt:


J Pharm Pharmacogn Res 6(1): 27-34, 2018. Original Article | Artículo Original IgM antibody and colony fungal load impacts of orally administered ethanol extract of Plectranthus scutellarioides on mice with systemic candidiasis [Impacto de la administración oral del extracto etanólico de Plectranthus scutellarioides sobre anticuerpos IgM y la carga fúngica en ratones con candidiasis sistémica] Marni Br. Karo1,2*, Mochammad … Continue reading IgM antibody and fungal load impacts of P. scutellarioides

J Pharm Pharmacogn Res 6(1): 27-34, 2018.

Original Article | Artículo Original

IgM antibody and colony fungal load impacts of orally administered ethanol extract of Plectranthus scutellarioides on mice with systemic candidiasis

[Impacto de la administración oral del extracto etanólico de Plectranthus scutellarioides sobre anticuerpos IgM y la carga fúngica en ratones con candidiasis sistémica]

Marni Br. Karo1,2*, Mochammad Hatta3, Ilhamjaya Patellongi4, Rosdiana Natzir5, Titus Tambaip6

1Midwifery Program of Medistra Health Higher School, Bekasi City, West Java, 17113, Indonesia.
2School of Post Graduate Faculty of Medicine, Hasanuddin University, Makassar City, South Sulawesi, 90245, Indonesia.
3Molecular Biology and Immunology Laboratory for Infection Diseases, Faculty of Medicine, Hasanuddin Makassar, Makassar City, South Sulawesi, 90245, Indonesia.
4Department of Physiology, Faculty of Medicine, Hasanuddin Makassar, Makassar City, South Sulawesi, 90245, Indonesia.
5Department of Biochemistry, Faculty of Medicine, Hasanuddin Makassar, Makassar City, South Sulawesi, 90245, Indonesia.
6Midwifery Program of Yaleka Maro School, Merauke City, Papua, 99613, Indonesia.

*E-mail: marnikaro.stikesmi@gmail.com

Abstract

Context: Candida albicans is a pathogenic fungus that causes vulvovaginal candidiasis (VVC), which presents significant clinical problems in immunocompromised patients. There are no studies that correlate IgM antibody responses and fungal loads following the use of ethanol leaf extract of Plectranthus scutellarioides (L.) R.Br. (ELEP) on VVC.

Aims: To examine colony fungi loads, IgM antibody responses, and their correlation in Balb/c mice infected with C. albicans treated with orally administered ELEP.

Methods: Twenty-five female Balb/c mice were randomly divided into five groups (five mice in each group): normal group (oral ELEP + not infected), VVC control (VCC) group (infected + oral 0.2 mL saline), ketoconazole-treated in VCC group (infected + oral antifungal agents), and two treatment groups (infected + oral ELEP with 500 and 750 mg/kg b.w.). Treatments were administered for 14 days after infection with C. albicans. Colony fungal loads and IgM antibodies were measured by counting colony forming units and flow cytometry using anti-C. albicans antibody kits, respectively.

Results: Ingestion of the ELEP significantly affected fungal load clearance and decreased IgM antibody levels compared to that in the VCC group (p<0.001). Furthermore, ingestion of the ELEP was associated with significantly lower concentrations of IgM antibodies and colony forming units (p<0.001).

Conclusions: These data strongly suggest that IgM antibody response is involved in of the mechanism by which ELEP decreases C. albicans infection. Furthermore, these results provide important information for the potential application of ELEP in the treatment of Balb/C mice with systemic candidiasis.

Keywords: anti-candida; immune response; miana leaves; vulvovaginal candidiasis.

Resumen

Contexto: Candida albicans es un hongo patógeno que causa candidiasis vulvovaginal (VVC), que presenta problemas clínicos significativos en pacientes inmunocomprometidos. No existen estudios que correlacionen las respuestas de anticuerpos IgM y cargas fúngicas después del uso del extracto etanólico de hoja de Plectranthus scutellarioides (L.) R.Br. (ELEP) en VVC.

Objetivos: Examinar la carga fúngica de colonias, la respuesta de anticuerpos IgM y su correlación en ratones Balb/c infectados con C. albicans tratados con ELEP administrado por vía oral.

Métodos: Veinticinco ratones Balb/c hembras se dividieron en cinco grupos (cinco ratones/grupo): grupo normal (ELEP oral no infectado), grupo control VVC (infectado + 0,2 mL salino oral), tratado con ketoconazol en el grupo VCC (VVC + agentes antifúngicos orales) y dos grupos de tratamiento (VVC + ELEP oral con 500 y 750 mg/kg p.c.). Los tratamientos se administraron durante 14 días después de la infección con C. albicans. Las cargas fúngicas y los anticuerpos IgM se midieron contando unidades formadoras de colonias y citometría de flujo usando kits de anticuerpos anti-C. albicans, respectivamente.

Resultados: La ingestión de ELEP afectó el aclaramiento de la carga fúngica y la disminución de los niveles de anticuerpos IgM en comparación con el grupo VCC (p<0,001). Además, la ingestión de ELEP se asoció con concentraciones más bajas de anticuerpos IgM y unidades formadoras de colonias (p<0,001).

Conclusiones: Estos datos sugieren que la respuesta de anticuerpos IgM está implicada en el mecanismo por el cual ELEP disminuye la infección por C. albicans. Además, estos resultados proporcionan información importante para la aplicación potencial de ELEP en el tratamiento de ratones Balb/c con candidiasis sistémica.

Palabras Clave: anti-candida; candidiasis vulvovaginal; hojas de miana; respuesta inmune.

Download the PDF file .

 

 

Citation Format: Karo M, Hatta M, Patellongi I, Natzir T, Tambaip T (2018) IgM antibody and colony fungal load impacts of orally administered ethanol extract of Plectranthus scutellarioides on mice with systemic candidiasis. J Pharm Pharmacogn Res 6(1): 27–34.

© 2018 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

System optimization and validation of roselle
J. Pharm. Pharmacogn. Res., vol. 11, no. 2, pp. 243-254, March-April 2023. DOI: https://doi.org/10.56499/jppres22.1566_11.2.243 Original Article System optimization and validation to improve thin-layer chromatography of roselle calyces (Hibiscus sabdariffa L.) required by the Indonesian Herbal Pharmacopoeia Edition II [Optimización y validación del sistema para mejorar la cromatografía en capa fina de cálices de rosa mosqueta (Hibiscus … Continue reading System optimization and validation of roselle
Chitosan-alginate scaffold with Zingiber officinale for neural tissue engineering
J. Pharm. Pharmacogn. Res., vol. 11, no. 2, pp. 229-242, March-April 2023. DOI: https://doi.org/10.56499/jppres22.1505_11.2.229 Original Article Chitosan–alginate porous scaffold incorporated with hydroalcoholic Zingiber officinale Roscoe extract for neural tissue engineering [Estructura porosa de quitosano-alginato incorporada con extracto hidroalcohólico de Zingiber officinale Roscoe para la ingeniería del tejido neural] Hassan Sohrabian Kafraj1, Maryam Alipour2, Abdolhosein Shiravi1, Vida … Continue reading Chitosan-alginate scaffold with Zingiber officinale for neural tissue engineering
Ilex paraguariensis and eggshell membrane vs. aging
J. Pharm. Pharmacogn. Res., vol. 11, no. 2, pp. 216-228, March-April 2023. DOI: https://doi.org/10.56499/jppres22.1509_11.2.216 Original Article Oral supplementation of Ilex paraguariensis extract and eggshell membrane exhibit beneficial effects on the skin of mature male rats [La suplementación oral de extracto de Ilex paraguariensis y membrana de cáscara de huevo muestran efectos beneficiosos sobre la piel de … Continue reading Ilex paraguariensis and eggshell membrane vs. aging

© 2013-2020 by the authors; licensee JPPRes, Antofagasta, Chile. This journal is an open-access journal distributed under the terms and conditions of the Creative Commons Attribution license-Non Commercial 4.0 international. The content on this site is intended for health professionals. If you are not a health professional, please talk to your doctor about any doubts or concerns regarding your health

Made with ♥ by AVAGAX Studio