Immunomodulator effect of Parkia speciosa

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J Pharm Pharmacogn Res 8(5): 457-465, 2020. DOI: https://doi.org/10.56499/jppres20.826_8.5.457 Original Article Immunomodulatory effect of Parkia speciosa Hassk. pods extract on rat induced by Salmonella typhimurium [Efecto inmunomodulador del extracto de vainas de Parkia speciosa Hassk. en ratas inducidas por Salmonella typhimurium] Fitrya Fitrya1*, Annisa Amriani1, Rennie Puspa Novita1, Elfita2, Dewi Setiorini1 1Pharmacy Department, Sriwijaya University, Palembang, Indonesia. … Continue reading Immunomodulator effect of Parkia speciosa

J Pharm Pharmacogn Res 8(5): 457-465, 2020.

DOI: https://doi.org/10.56499/jppres20.826_8.5.457

Original Article

Immunomodulatory effect of Parkia speciosa Hassk. pods extract on rat induced by Salmonella typhimurium

[Efecto inmunomodulador del extracto de vainas de Parkia speciosa Hassk. en ratas inducidas por Salmonella typhimurium]

Fitrya Fitrya1*, Annisa Amriani1, Rennie Puspa Novita1, Elfita2, Dewi Setiorini1

1Pharmacy Department, Sriwijaya University, Palembang, Indonesia.
2Chemistry Department, Sriwijaya University, Palembang, Indonesia.
Abstract

Context: Parkia speciosa, which has phenolic compounds and pharmacology activities, is a plant having potential immunomodulatory agents from the extract of its pod.

Aims: To evaluate the potential of P. speciosa as an immunomodulatory agent tested on albino rats induced by the bacteria Salmonella typhimurium leading to typhoid fever.

Methods: The animals were divided into six groups: normal non-infected group, negative control group, positive control, and three test groups with doses of 200, 400 and 800 mg/kg BW. The test groups were treated by the extract for 12 days. The control and test groups were induced with the S. typhimurium on the 8th day. On day 12th, CD4+, total leukocytes, differential cell count, and histology of the spleen organ were evaluated. Statistical analysis was performed by using one-way ANOVA followed post hoc LSD test (α 0.05).

Results: The result showed that the ethanol extract of P. speciosa pod could increase the number of CD4+. The extract to 200 and 400 mg/kg BW doses increased in the parameters of leukocyte, neutrophil, lymphocyte and monocyte with insignificant differences compared to that of the positive control group (p>0.05). However, there were significant differences between the two first parameters between the test group with 800 mg/kg BW doses compared to the positive control group. Moreover, more necroses in the spleen were observed from histological analysis on 800 mg/kg BW doses than 400 mg/kg BW.

Conclusions: In general, P. speciosa ethanolic extract can increase the immune system based on the CD4+ count, leukocyte, monocyte, and neutrophil, and causes normal necroses in low doses.

Keywords: Parkia speciosa; immunomodulator; Salmonella typhimurium; typhoid fever.

Resumen

Contexto: Parkia speciosa, que tiene compuestos fenólicos y actividades farmacológicas, es una planta que tiene agentes inmunomoduladores potenciales del extracto de su vaina.

Objetivos: Evaluar el potencial de P. speciosa como agente inmunomodulador en ratas albinas inducidas por la bacteria Salmonella typhimurium que conduce a la fiebre tifoidea.

Métodos: Los animales se dividieron en seis grupos: grupo normal no infectado, grupo de control negativo, control positivo y tres grupos de prueba con dosis de 200, 400 y 800 mg/kg de peso corporal. Los grupos de prueba fueron tratados con el extracto durante 12 días. El control y los grupos de prueba fueron inducidos con S. typhimurium en el octavo día. El día 12, se evaluaron los CD4+, los leucocitos totales, el recuento diferencial de células y la histología del órgano del bazo. El análisis estadístico se realizó mediante el uso de ANOVA unidireccional seguido de la prueba post hoc LSD (α 0,05).

Resultados: El extracto etanólico de la vaina de P. speciosa podría aumentar el número de CD4+. El extracto a dosis de 200 y 400 mg/kg de peso corporal aumentó en los parámetros de leucocitos, neutrófilos, linfocitos y monocitos con diferencias insignificantes en comparación con el grupo de control positivo (p>0,05). Sin embargo, hubo diferencias significativas entre los dos primeros parámetros entre el grupo de prueba con dosis de 800 mg kg de peso corporal en comparación con el grupo de control positivo. Además, se observaron más necrosis en el bazo a partir del análisis histológico con dosis de 800 mg/kg de peso corporal que con 400 mg/kg de peso corporal.

Conclusiones: En general, el extracto etanólico de P. speciosa puede aumentar el sistema inmunitario en función del recuento de CD4+, leucocitos, monocitos y neutrófilos, y causa necrosis normales en dosis bajas.

Palabras Clave: Parkia speciosa; inmunomodulador; Salmonella typhimurium; fiebre tifoidea.

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Citation Format: Fitrya F, Amriani A, Novita RP, Elfita, Setiorini D (2020) Immunomodulatory effect of Parkia speciosa Hassk. pods extract on rat induced by Salmonella typhimurium. J Pharm Pharmacogn Res 8(5): 457–465. DOI: https://doi.org/10.56499/jppres20.826_8.5.457

© 2020 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

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