Immunostimulatory effect of a nature-derived capsule

Excerpt:


J. Pharm. Pharmacogn. Res., vol. 12, no. 5, pp. 892-899, Sep-Oct 2024. DOI: https://doi.org/10.56499/jppres23.1751_12.5.892 Original Article Potential effects of Linh Loc Son hard capsule – a Vietnamese herbal combination in immunodeficiency induced by cyclophosphamide on mice [Efectos potenciales de la cápsula dura Linh Loc Son, una combinación de hierbas vietnamitas en la inmunodeficiencia inducida por … Continue reading Immunostimulatory effect of a nature-derived capsule

J. Pharm. Pharmacogn. Res., vol. 12, no. 5, pp. 892-899, Sep-Oct 2024.

DOI: https://doi.org/10.56499/jppres23.1751_12.5.892

Original Article

Potential effects of Linh Loc Son hard capsule – a Vietnamese herbal combination in immunodeficiency induced by cyclophosphamide on mice

[Efectos potenciales de la cápsula dura Linh Loc Son, una combinación de hierbas vietnamitas en la inmunodeficiencia inducida por ciclofosfamida en ratones]

Binh Quoc Pham1, Ngan Kim Thi Nguyen1, Su Quoc Pham1, Cuong Duy Nguyen2, Lam Vu Trinh1, Hang Thu Thi Dinh3, Quang Vinh Trinh3, Van Anh Thi Pham3*

1Vietnam University of  Traditional Medicine, Hanoi, Vietnam.

2Department of Health, Vietnam Ministry of Public Security, Hanoi, Vietnam.

3Hanoi Medical University, Hanoi, Vietnam.

*E-mail: phamvananh@hmu.edu.vn

Abstract

Context: Traditional medicine and herbal extracts have long been recognized for their immunomodulatory effects, enhancing patient immune responses. The use of traditional medicine to target specific pathogens and support the immune system is increasingly studied. Linh Loc Son (LLS) hard capsule, a product derived from nature, consists of the main four natural ingredients: Curculigo orchioides Gaertn., Dioscorea persimilis Prain et Burk., Morinda officinalis F.C.How. and Fallopia multiflora (Thunb.) Haraldson.

Aims: To evaluate the potential effects of LLS hard capsules in complementary treatment of immunodeficiency state in mice.

Methods: An experiment was conducted to assess the impact of LLS hard capsules on an immunosuppressed model of cyclophosphamide-induced Swiss mice of either sex. Two doses of LLS hard capsules (0.69 and 1.38 g/kg body weight) and levamisole (100 mg/kg body weight), serving as a positive control, were administered orally for seven consecutive days; cyclophosphamide (200 mg/kg i.p.) was administered on the fourth day.

Results: Both doses of LLS hard capsule did insignificantly ameliorate the immunosuppressive effects of cyclophosphamide on the delayed-type hypersensitivity response but maintained leukocyte counts, relative organ weight, and cytokines as compared to the levamisole group and showed a significant improvement in micro-histological images.

Conclusions: This study has demonstrated in vivo the immunostimulatory effect of LLS hard capsules, highlighting their potential to boost and regulate the immune response in mice models of immunodeficiency.

Keywords: experimental; herbal medicine; immunologic; immunosuppression

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Resumen

Contexto: La medicina tradicional y los extractos de hierbas son reconocidos desde hace mucho tiempo por sus efectos inmunomoduladores, que mejoran las respuestas inmunitarias de los pacientes. Se estudia cada vez más el uso de la medicina tradicional para atacar patógenos específicos y apoyar el sistema inmunológico. La cápsula dura Linh Loc Son (LLS), un producto derivado de la naturaleza, consta de los cuatro ingredientes naturales principales: Curculigo orchioides Gaertn., Dioscorea persimilis Prain et Burk., Morinda officinalis F.C.How. y Fallopia multiflora (Thunb.) Haraldson.

Objetivos: Evaluar los efectos potenciales de la cápsula dura de LLS en el tratamiento complementario del estado de inmunodeficiencia en ratones.

Métodos: Se llevó a cabo un experimento para evaluar el impacto de las cápsulas duras de LLS en un modelo inmunosuprimido de ratones suizos de ambos sexos inducidos por ciclofosfamida. Se administraron por vía oral dos dosis de cápsulas duras de LLS (0,69 y 1,38 g/kg de peso corporal) y levamisol (100 mg/kg de peso corporal), que sirvieron como control positivo, por vía oral durante siete días consecutivos; Se administró ciclofosfamida (200 mg/kg i.p.) el cuarto día.

Resultados: Ambas dosis de la cápsula dura de LLS mejoraron de manera insignificante los efectos inmunosupresores de la ciclofosfamida en la respuesta de hipersensibilidad de tipo retardado, pero mantuvieron los recuentos de leucocitos, el peso relativo de los órganos y las citocinas en comparación con el grupo de levamisol y mostraron una mejora significativa en las imágenes microhistológicas.

Conclusiones: Este estudio ha demostrado in vivo el efecto inmunoestimulador de la cápsula dura de LLS, destacando su potencial para estimular y regular la respuesta inmune en un modelo de inmunodeficiencia en ratones.

Palabras Clave: experimental; medicina herbaria; inmunológico; inmunosupresión.

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Citation Format: Binh QP, Ngan NTK, Su QP, Cuong ND, Lam TV, Hang DTT, Quang TV, Van Anh PT (2024) Potential effects of Linh Loc Son hard capsule – a Vietnamese herbal combination in immunodeficiency induced by cyclophosphamide on mice. J Pharm Pharmacogn Res 12(5): 892–899. https://doi.org/10.56499/jppres23.1751_12.5.892
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