In silico and in vitro neuroprotective flavonoids for Alzheimer

Excerpt:


J. Pharm. Pharmacogn. Res., vol. 12, no. 2, pp. 204-217, Mar-Apr 2024. DOI: https://doi.org/10.56499/jppres23.1715_12.2.204 Original Article Uncovering potential neuroprotective flavonoids for Alzheimer's disease using cutting-edge molecular simulation and in vitro SHSY-5Y analysis [Descubrimiento de posibles flavonoides neuroprotectores para la enfermedad de Alzheimer mediante simulación molecular de vanguardia y análisis SHSY-5Y in vitro] Fadilah Fadilah1,3*, Immanuelle … Continue reading In silico and in vitro neuroprotective flavonoids for Alzheimer

J. Pharm. Pharmacogn. Res., vol. 12, no. 2, pp. 204-217, Mar-Apr 2024.

DOI: https://doi.org/10.56499/jppres23.1715_12.2.204

Original Article

Uncovering potential neuroprotective flavonoids for Alzheimer's disease using cutting-edge molecular simulation and in vitro SHSY-5Y analysis

[Descubrimiento de posibles flavonoides neuroprotectores para la enfermedad de Alzheimer mediante simulación molecular de vanguardia y análisis SHSY-5Y in vitro]

Fadilah Fadilah1,3*, Immanuelle Kezia2, Linda Erlina1,3

1Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

2Master’s Programme in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

3Bioinformatics Core Facilities, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

*E-mail: fadilah.msi@ui.ac.id

Abstract

Context: Alzheimer's disease (AD) is a debilitating neurodegenerative condition primarily afflicting the elderly, causing a progressive decline in cognitive function. It is marked by the presence of beta-amyloid protein plaques that trigger inflammation and neuronal death. Unfortunately, effective treatments for Alzheimer's remain elusive. One promising approach involves targeting the mitochondrial cascade to shield neurons from inflammation-induced cell death, and flavonoids have been identified for their potential neuroprotective properties.

Aims: To investigate the most potential bioactive compound that has neuroprotective effects, especially for Alzheimer's, through molecular simulation and in vitro studies.

Methods: The study employed molecular simulations to identify apigenin as a potential neuroprotective compound. Quercetin and donepezil were selected as control compounds. The MTT assay was conducted to evaluate the neuroprotective activity of apigenin, quercetin, and donepezil on the SHSY5Y cell line induced by beta-amyloid protein.

Results: The MTT assay demonstrated the neuroprotective activity of apigenin and reference compounds quercetin and donepezil against beta-amyloid-induced damage in the SHSY5Y cell line.

Conclusions: These findings suggest that apigenin could hold promise as a neuroprotective treatment for AD. However, further research is essential to elucidate its mechanism of action and evaluate its efficacy in more complex organisms. This study underscores the crucial role of bioinformatics tools and experimental validation in the quest for potential neuroprotective compounds for the treatment of AD.

Keywords: Alzheimer; bioinformatics; flavonoid; neuroprotective; SHSY-5Y cell line.

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Resumen

Contexto: La enfermedad de Alzheimer (EA) es una patología neurodegenerativa debilitante que afecta principalmente a las personas mayores y provoca un deterioro progresivo de la función cognitiva. Se caracteriza por la presencia de placas de proteína beta-amiloide que desencadenan inflamación y muerte neuronal. Por desgracia, los tratamientos eficaces siguen siendo difíciles de encontrar. Un enfoque prometedor implica dirigirse a la cascada mitocondrial para proteger a las neuronas de la muerte celular inducida por la inflamación, y se han identificado flavonoides por sus potenciales propiedades neuroprotectoras.

Objetivos: Investigar mediante simulación molecular y estudios in vitro el compuesto bioactivo con mayor potencial neuroprotector, especialmente para la enfermedad de Alzheimer.

Métodos: El estudio empleó simulaciones moleculares para identificar la apigenina como compuesto neuroprotector potencial. La quercetina y el donepezilo se seleccionaron como compuestos de control. Se realizó el ensayo MTT para evaluar la actividad neuroprotectora de la apigenina, la quercetina y el donepezilo en la línea celular SHSY5Y inducida por la proteína beta-amiloide.

Resultados: El ensayo MTT demostró la actividad neuroprotectora de la apigenina y de los compuestos de referencia quercetina y donepezilo frente al daño inducido por beta-amiloide en la línea celular SHSY5Y.

Conclusiones: Estos resultados sugieren que la apigenina podría ser prometedora como tratamiento neuroprotector para la EA. Sin embargo, es esencial seguir investigando para dilucidar su mecanismo de acción y evaluar su eficacia en organismos más complejos. Este estudio subraya el papel crucial de las herramientas bioinformáticas y la validación experimental en la búsqueda de potenciales compuestos neuroprotectores para el tratamiento de la EA.

Palabras Clave: Alzheimer; bioinformática; flavonoide; línea celular SHSY-5Y; neuroprotector.

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Citation Format: Fadilah F, Kezia I, Erlina L (2024) Uncovering potential neuroprotective flavonoids for Alzheimer's disease using cutting-edge molecular simulation and in vitro SHSY-5Y analysis. J Pharm Pharmacogn Res 12(2): 204–217. https://doi.org/10.56499/jppres23.1715_12.2.204
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