In vivo and in vitro effects of medicinal herbs on erythrocytes



J Pharm Pharmacogn Res 9(4): 422-434, 2021.

Original article

Effects of commonly used medicinal herbs in Jordan on erythrocyte oxidative stress markers

[Efectos de las hierbas medicinales de uso común en Jordania sobre los marcadores antioxidantes de eritrocitos]

Yousif Y. Bilto1*, Nessrin G.Alabdallat2, Ali M. Atoom3, Noaman A. Khalaf4

1Department of Clinical Laboratory Sciences. The University of Jordan, Amman 11942, Jordan.

2Department of Medical Laboratory Sciences. Majmaah University, Majmaah, Saudi Arabia.

3Department of Medical Laboratory Sciences. AL-Ahliyya Amman University, Amman, Jordan.

4Department of pharmacy. AL-Ahliyya Amman University, Amman, Jordan.

*E-mail: bilto@ju.edu.jo

Abstract

Context: Much attention has been given recently to the antioxidant capacity of natural products, with particular interest on those that are frequently consumed by people.

Aims: To evaluate the commonly used and frequently consumed edible herbs in Jordan to compare their in vitro and in vivo antioxidant properties.

Methods: The in vitro antioxidant properties were tested by pre-incubating washed human erythrocytes with a given herb extract and then exposing these erythrocytes to H2O2 to induce oxidative stress, and then measuring erythrocyte malondialdehyde (MDA) as a marker for lipid peroxidation, protein carbonyl (PC) as a marker for protein oxidation, reduced glutathione (GSH) as a marker for cellular antioxidant status and the percentage of hemolysis as an indicator for the anti-hemolytic activity of the herb. The in vivo antioxidant properties were tested by giving orally aqueous extracts of the herbs tested in vitro to healthy individuals on daily bases for five days, with two blood samples being collected from each individual to measure the above-mentioned markers.

Results: Pre-incubation of human erythrocytes in vitro with methanolic extracts of Zingiber officinale, Rosmarinus officinalis, Salvia triloba, Verbena triphylla, Nigella sativa and Origanum syriacum significantly improved erythrocyte MDA, PC and oxidant hemolysis. Oral consumption by healthy individuals of aqueous extracts of the same herbs for 5 days significantly improved erythrocyte MDA, GSH, and superoxide dismutase (SOD) at the sixth day of administration.

Conclusions: These results indicate that aqueous extracts of medicinal herbs can be absorbed well and appear in body tissues inflecting in vivo antioxidant properties similar to their in vitro properties.

Keywords: in vitro; in vivo; malondialdehyde; protein carbonyl; reduced glutathione; superoxide dismutase.

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Resumen

Contexto: Recientemente se ha prestado mucha atención a la capacidad antioxidante de los productos naturales, con especial interés en aquellos que son consumidos frecuentemente por las personas.

Objetivos: Evaluar las hierbas comestibles de uso común y de consumo frecuente en Jordania para comparar sus propiedades antioxidantes in vitro e in vivo.

Métodos: Las propiedades antioxidantes in vitro se probaron preincubando eritrocitos humanos lavados con un extracto de hierba dado y luego exponiendo estos eritrocitos a H2O2 para inducir estrés oxidativo, y luego midiendo el malondialdehído eritrocitario (MDA) como marcador de peroxidación de lípidos, proteína carbonilo (PC) como marcador de oxidación de proteínas, glutatión reducido (GSH) como marcador del estado antioxidante celular y el porcentaje de hemólisis como indicador de la actividad anti-hemolítica de la hierba. Las propiedades antioxidantes in vivo se probaron administrando, diariamente durante cinco días, extractos acuosos por vía oral de las hierbas probadas in vitro a individuos sanos, recogiéndose dos muestras de sangre de cada individuo para medir los marcadores mencionados anteriormente.

Resultados: La preincubación in vitro de eritrocitos humanos con extractos metanólicos de Zingiber officinale, Rosmarinus officinalis, Salvia triloba, Verbena triphylla, Nigella sativa y Origanum syriacum mejoró significativamente los niveles de MDA de eritrocitos, PC y hemólisis oxidante. El consumo oral por individuos sanos de extractos acuosos de las mismas hierbas durante 5 días mejoró significativamente MDA, GSH y superóxido dismutasa (SOD) de eritrocitos en el sexto día de administración.

Conclusiones: Estos resultados indican que los extractos acuosos de hierbas medicinales pueden absorberse bien y aparecer en los tejidos corporales con propiedades antioxidantes in vivo similares a sus propiedades in vitro.

Palabras Clave: carbonilo de proteína; glutatión reducido; in vitro; in vivo; malondialdehído; superóxido dismutasa.

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Citation Format: Bilto YY, Alabdallat NG, Atoom AM, Khalaf NA (2021) Effects of commonly used medicinal herbs in Jordan on erythrocyte oxidative stress markers. J Pharm Pharmacogn Res 9(4): 422–434.

© 2021 Journal of Pharmacy & Pharmacognosy Research (JPPRes)