In vivo bronchodilation of Jatropha macrantha



J Pharm Pharmacogn Res 9(6): 937-946, 2021.

Original article

In vivo bronchodilator evaluation of the ethanolic extract of the stems of Jatropha macrantha Müll.Arg.

[Evaluación broncodilatadora in vivo del extracto etanólico de los tallos de Jatropha macrantha Müll.Arg.]

Haydee Chávez1, Freddy Palomino1, Jackeline Angelino1, Ernesto Torres1, María R. Bendezú1, Jorge A. García1, Berta Loja1, Ana M. Muñoz2, Angel T. Alvarado3*

1Faculty of Pharmacy and Biochemistry, San Luis Gonzaga National University of Ica, Ica, Peru.

2Research Unit in Nutrition, Health, Functional Foods and Nutraceuticals, San Ignacio de Loyola University, Lima, Peru.

3International Research Network of Pharmacology and Precision Medicine (REDIFMEP), San Ignacio de Loyola University, Lima, Peru.

*E-mail: angel.alvaradoy@usil.pe

Abstract

Context: Asthma is characterized by hyperresponsiveness and bronchoconstriction, induced by histamine and other noxes, with a prevalence in Peru of 20.7-28.2%, which is why, in traditional medicine, Jatropha macrantha Müll.Arg is used.

Aims: To evaluate the in vivo bronchodilator activity of the ethanolic extract of the stems of J. macrantha.

Methods: The powdered material of the stem of J. macrantha was extracted with ethanol 96° by maceration; then, the phytochemical screening was carried out. The doses of 200, 400 and 800 mg/kg of the extracts were subjected to in vivo evaluation in guinea pigs (Cavia porcellus) with 1% histamine-induced bronchoconstriction, determining the latency period and the intensity of the effect.

Results: Phytochemical sieving showed alkaloids, catechins, flavonoids, tannins and terpenes. The activity was observed at the three doses of the J. macrantha extract, being higher at a single dose of 400 mg/kg, with a latency period of 33.0 ± 1.02 s, asphyxia 84.5 ± 1.08 s and anaphylaxis 105.6 ± 0.59 s. The analysis of variance and Dunnett’s test resulted with p<0.05 as statistically significant.

Conclusions: The results showed bronchodilator activity of the ethanolic extract of J. macrantha at an average dose of 400 mg/kg, orally, in Cavia porcellus.

Keywords: Jatropha macrantha; huanarpo female; bronchodilator activity.

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Resumen

Contexto: El asma se caracteriza por la hiperreactividad y la broncoconstricción, inducida por la histamina y otras noxas, con una prevalencia en el Perú del 20,7-28,2%, por lo que, en la medicina tradicional, se utiliza la J. macrantha.

Objetivos: Evaluar la actividad broncodilatadora in vivo del extracto etanólico de los tallos de J. macrantha.

Métodos: El material en polvo del tallo de J. macrantha se extrajo con etanol 96° por maceración; luego, se realizó el tamizaje fitoquímico. Las dosis de 200, 400 y 800 mg/kg de los extractos se sometieron a evaluación in vivo en cobayos (Cavia porcellus) con broncoconstricción inducida con histamina 1%, determinándose el período de latencia y la intensidad del efecto.

Resultados: Al tamizado fitoquímico se observó alcaloides, catequinas, flavonoides, taninos y terpenos. Se observó actividad a las tres dosis del extracto de Jatropha, siendo mayor a dosis única de 400 mg/kg, con un período de latencia de 33,0 ± 1,02 s), asfixia 84.5 ± 1,08 s y anafilaxia 105,6 ± 0,59 s. El análisis de varianza y la prueba de Dunnett’s resultó con p<0,05 como estadísticamente significativo.

Conclusiones: Los resultados mostraron actividad broncodilatadora del extracto etanólico de tallos de J. macrantha a una dosis media de 400 mg/kg, por vía oral, en Cavia porcellus.

Palabras Clave: Jatropha macrantha; huanarpo hembra; actividad broncodilatadora.

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Citation Format: Chávez H, Palomino F, Angelino J, Torres E, Bendezú MR, García JA, Loja B, Muñoz AM, Alvarado AT (2021) In vivo bronchodilator evaluation of the ethanolic extract of the stems of Jatropha macrantha Müll.Arg. J Pharm Pharmacogn Res 9(6): 937–946.

© 2021 Journal of Pharmacy & Pharmacognosy Research (JPPRes)