Lemongrass oil nanoparticle as antimicrobial and its acrylic resin hardness influence

Excerpt:


J. Pharm. Pharmacogn. Res., vol. 12, no. 6, pp. 1156-1169, Nov-Dec 2024. DOI: https://doi.org/10.56499/jppres23.1935_12.6.1156 Original Article Lemongrass (Cymbopogon citratus) oil nanoparticle synthesis, characteristic, and evaluation of antibacterial and antifungal effects and the influence on hardness of acrylic resin [Síntesis de nanopartículas de aceite de limoncillo (Cymbopogon citratus), características y evaluación de los efectos antibacterianos y … Continue reading Lemongrass oil nanoparticle as antimicrobial and its acrylic resin hardness influence

J. Pharm. Pharmacogn. Res., vol. 12, no. 6, pp. 1156-1169, Nov-Dec 2024.

DOI: https://doi.org/10.56499/jppres23.1935_12.6.1156

Original Article

Lemongrass (Cymbopogon citratus) oil nanoparticle synthesis, characteristic, and evaluation of antibacterial and antifungal effects and the influence on hardness of acrylic resin

[Síntesis de nanopartículas de aceite de limoncillo (Cymbopogon citratus), características y evaluación de los efectos antibacterianos y antifúngicos y la influencia sobre la dureza de la resina acrílica]

Vinna K. Sugiaman1*, Rosalina Intan Saputri2, Silvia Naliani3, Jane Amalia3, Jeffrey4

1Department of Oral Biology, Faculty of Dentistry, Maranatha Christian University, Bandung, West Java, Indonesia.

2Departement of Biomedical Science, Faculty of Dentistry, Maranatha Christian University, Bandung, West Java, Indonesia.

3Department of Prosthodontics, Faculty of Dentistry, Maranatha Christian University, Bandung, West Java, Indonesia.

4Department of Pediatric Dentistry, Faculty of Dentistry, Jenderal Achmad Yani University, Cimahi, West Java, Indonesia.

*E-mail: vinna.ks@dent.maranatha.edu

Abstract

Context: Acrylic resin is used in dentistry as a removable denture base. It can cause various pathologies when not properly cleaned. One of the pathologies is denture stomatitis caused by Candida albicans and Streptococcus mutans accumulation on the acrylic resin surface. Therefore, microbial agents such as denture cleansers are needed.

Aims: To evaluate the characteristics of lemongrass (Cymbopogon citratus) nanoparticles as a better antibacterial and antifungal herbal ingredient and their relationship with acrylic hardness.

Methods: C. citratus oil nanoparticles (LON) were synthesized and analyzed by transmission electron microscopy (TEM). Electrospray ionization tandem mass spectrometry (ESI-MS) analysis was used to analyze the characteristics of LON bioactive components. Minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) against C. albicans and S. mutans and mechanical hardness test of acrylic were performed.

Results: The LON concentration of MIC and MBC against C. albicans and S. mutans was 25 and 100%, respectively. One-way ANOVA showed no significant difference between groups of LON with different concentrations (p=0.687). A paired t-test showed significant differences in acrylic resin hardness before and after treatment of LON with 100% (p=0.022) and 50% (p=0.021) concentration. There was no significant difference in hardness before and after treatment of other concentrations of LON and chlorhexidine as positive control.

Conclusions: LON treatment on acrylic resin decreased the growth of C. albicans and S. mutans without altering the mechanical properties (hardness).

Keywords:acrylic resin; Candida albicans; Cymbopogon citratus; nanoparticle; Streptococcus mutans.

PDF Download

Resumen

Contexto: La resina acrílica se utiliza en odontología como base para prótesis removibles. Puede provocar diversas patologías si no se limpia adecuadamente. Una de las patologías es la estomatitis de la prótesis dental causada por la acumulación de Candida albicans y Streptococcus mutans en la superficie de la resina acrílica. Por lo tanto, se necesitan agentes microbianos como limpiadores de dentaduras postizas.

Objetivos: Evaluar las características de las nanopartículas de hierba limón (Cymbopogon citratus) como mejor ingrediente herbario antibacteriano y antifúngico y su relación con la dureza del acrílico.

Métodos: Se sintetizaron y analizaron nanopartículas de aceite de C. citratus (LON) mediante microscopía electrónica de transmisión (TEM). Se utilizó el análisis de espectrometría de masas en tándem de ionización por electropulverización (ESI-MS) para analizar las características de los componentes bioactivos de LON. Se realizaron Concentraciones Mínimas Inhibitorias (CIM) y Concentraciones Mínimas Bacterianas (CBM) contra C. albicans y S. mutans y pruebas de dureza mecánica del acrílico.

Resultados: La concentración de LON de MIC y MBC contra C. albicans y S. mutans fue del 25 y 100%, respectivamente. El ANOVA unidireccional no mostró diferencias significativas entre los grupos de LON con diferentes concentraciones (p=0,687). Una prueba t pareada mostró diferencias significativas en la dureza de la resina acrílica antes y después del tratamiento con LON con una concentración del 100% (p=0,022) y del 50% (p=0,021). No hubo diferencias significativas en la dureza antes y después del tratamiento con otras concentraciones de LON y clorhexidina como control positivo.

Conclusiones: El tratamiento con LON sobre resina acrílica disminuyó el crecimiento de C. albicans y S. mutans sin alterar las propiedades mecánicas (dureza).

Palabras Clave: resina acrílica; Candida albicans; Cymbopogon citratus; nanopartícula; Streptococcus mutans.

PDF Download

 

 
 
Citation Format: Sugiaman VK, Saputri RI, Naliani S, Amalia J, Jeffrey (2024) Lemongrass (Cymbopogon citratus) oil nanoparticle synthesis, characteristic, and evaluation of antibacterial and antifungal effects and the influence on hardness of acrylic resin. J Pharm Pharmacogn Res 12(6): 1156–1169. https://doi.org/10.56499/jppres23.1935_12.6.1156
References

Abdurahman SNS, Zulkifli NMFAN, Ghafar SAA, Abdullah SN (2020) Biofilm formation between species associated with denture stomatitis. Dent Oral Biol Craniofacial Res 3(6): 1–3. http://dx.doi.org/10.31487/j.DOBCR.2020.06.02

Ahmad N, Ahmad R, Al-Qudaihi A, Alaseel SE, Fita IZ, Khalid MS, Pottoo FH (2019) Preparation of a novel curcumin nanoemulsion by ultrasonication and its comparative effects in wound healing and the treatment of inflammation. RSC Adv 9(35): 20192–20206. https://doi.org/10.1039/C9RA03102B

Ajayi E, Afolayan A (2017) Green synthesis, characterization and biological activities of silver nanoparticles from alkalinized Cymbopogon citratus Stapf. Adv Nat Sci Nanosci Nanotechnol 8(1): 015017. https://doi.org/10.1088/2043-6254/aa5cf7

Akın G, Arslan FN, Elmas Karuk ŞN, Yılmaz İ (2018) Cold-pressed pumpkin seed (Cucurbita pepo L.) oils from the central Anatolia region of Turkey: Characterization of phytosterols, squalene, tocols, phenolic acids, carotenoids and fatty acid bioactive compounds. Grasas Aceites 69(1): e232. https://doi.org/10.3989/gya.0668171

Al-Khafaji MA, Gaál A, Wacha A, Bóta A, Varga Z (2020) Particle size distribution of bimodal silica nanoparticles: A comparison of different measurement techniques. Materials 13(14): 3101. https://doi.org/10.3390/ma13143101

Alqutaibi AY, Baik A, Almuzaini SA, Farghal AE, Alnazzawi AA, Borzangy S, Aboalrejal AN, AbdElaziz MH, Mahmoud II, Zafar MS (2023) Polymeric denture base materials: A review. Polymers 15(15): 3258. https://doi.org/10.3390/polym15153258

An S, Judge RB, Wong RH, Arzmi MH, Palamara JE, Dashper SG (2018) Incorporation of the microencapsulated antimicrobial agent phytoncide into denture base resin. Aust Dent J 63(3): 302–311. https://doi.org/10.1111/adj.12640

Archilla AR, Galan CG (2020) Etiological factors related to denture stomatitis: A meta‑analysis. Dent Med Res 8: 37–42. https://doi.org/10.4103/dmr.dmr_26_20

Arthington-Skaggs BA, Yang WL, Ciblak MA, Frade JP, Brandt ME, Hajjeh RA, Harrison LH, Sofair AN, Warnock DW (2002) Comparison of visual and spectrophotometric methods of broth microdilution MIC end point determination and evaluation of a sterol quantitation methods for in vitro susceptibility testing of fluconazole and itraconazole against trailing and nontrailing Candida isolates. Antimicrob Agents Chemother 46(8): 2477–2481. https://doi.org/10.1128/AAC.46.8.2477-2481.2002

Ayavoo T, Murugesan K, Gnanasekaran A (2021) Roles and mechanisms of stem cell in wound healing. Stem Cell Investig 8: 4. https://doi.org/10.21037/sci-2020-027

Ayaz EA, Durkan R, Koroglu A, Bagis B (2014) Comparative effect of different polymerization techniques on residual monomer and hardness properties of PMMA-based denture resins. J Appl Biomater Funct Mater 12(3): 228–233. https://doi.org/10.5301/jabfm.5000199

Azghar A, Dalli M, Loukili EH, Belbachir Y, Tahri M, Benaissa E, Lahlou YB, Elouennass M, Maleb A (2023) Evaluation of the antibacterial activity of essential oil of Dysphania ambrosioides (L.) Mosyakin and Clemants against clinical multidrug-resistant bacteria. Asian J Plant Sci 22(1): 75–81. https://doi.org/10.3923/ajps.2023.75.81

Bajunaid SO (2022) How effective are antimicrobial agents on preventing the adhesion of Candida albicans to denture base acrylic resin materials? A systematic review. Polymers 14(5): 908. https://doi.org/10.3390/polym14050908

Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 6(2): 71–79. https://doi.org/10.1016/j.jpha.2015.11.005

Bansal P, Sharma A, Bhanot R, Chahal G (2013) Denture stomatitis an underlying menace. Dent J Adv Studies 01(01): 33–36. https://doi.org/10.1055/S-0038-1670591

Baranwal A, Srivastava A, Kumar P, Bajpai VK, Maurya PK, Chandra P (2018) Prospects of nanostructure materials and their composites as antimicrobial agents. Front Microbiol 9: 422. https://doi.org/10.3389/fmicb.2018.00422

Barua DR, Basavanna JM, Varghese RK (2017) Efficacy of neem extract and three antimicrobial agents incorporated into tissue conditioner in inhibiting the growth of C. albicans and S. mutans. J Clin Diagnostic Res 11(5): ZC97–ZC101. https://doi.org/10.7860/JCDR/2017/23784.9950

Basera P, Lavania M, Agnihotri A, Lal B (2019) Analytical investigation of Cymbopogon citratus and exploiting the potential of developed silver nanoparticle against the dominating species of pathogenic bacteria. Front Microbiol 10: 282. https://doi.org/10.3389/fmicb.2019.00282

Bossou AFAD, Bogninou GSR, Agbangnan Dossa CP, Yedomonhan H, Avlessi F, Sohounhloué DCK (2020) Volatile profiles and biological properties of Cymbopogon citratus, Cymbopogon giganteus, Cymbopogon shoenanthus, and their isolated compounds: A review. J Biomed Pharm Res 9(1): 22–32. https://doi.org/10.32553/jbpr.v9i1.711

Bukhari MA, Algahtani MA, Alsuwailem FA, Alogaiel RM, Almubarak SH, Alqahtani SS (2022) Epidemiology, etiology, and treatment of denture stomatitis. Int J Community Med Public Health 9(2): 981–986. https://doi.org/10.18203/2394-6040.ijcmph20220003

Campos Sugio CY, Robles Mengoa MG, Gurgel Gomes AC, Neves Garcia AAM, Marchini de Oliveira Y, Hermana Neppelenbroek K (2020) Use of natural products in the prevention and treatment of denture stomatitis. Open Acc J Bio Sci 1(5): 201–206. https://doi.org/10.38125/oajbs.000146

Carvalho CF, Vanderlei AD, Marocho SMS, Pereira SMB, Nogueira L, Paes-Júnior TJA (2012) Effect of disinfectant solutions on a denture base acrylic resin. Acta Odontol Latinoam 25(3): 255–260. https://pubmed.ncbi.nlm.nih.gov/23798071/

Chen T, Yao Q, Nasaruddin RR, Xie J (2019) Electrospray ionization mass spectrometry: A powerful platform for noble‐metal nanocluster analysis. Angew Chemie 131(35): 12093–12103. https://doi.org/10.1002/ange.201901970

Cherian T, Ali K, Saquib Q, Faisal M, Wahab R, Musarrat J (2020) Cymbopogon citratus functionalized green synthesis of CuO-nanoparticles: Novel prospects as antibacterial and antibiofilm agents. Biomolecules 10(2): 169. https://doi.org/10.3390/biom10020169

Chlif N, Ed-Dra A, Diouri M, El Messaoudi N, Zekkori B, Filali FR, Bentayeb A (2021) Chemical composition, antibacterial and antioxidant activities of essential oils extracted from dry and fresh brocchia cinerea. Biodiversitas 22(4): 1741–1749. https://doi.org/10.13057/biodiv/d220418

Choonharuangdej S, Srithavaj T, Thummawanit S (2020) Fungicidal and inhibitory efficacy of cinnamon and lemongrass essential oils on Candida albicans biofilm established on acrylic resin: An in vitro study. J Prosthet Dent 125(4): 707.e1–707.e6. https://doi.org/10.1016/j.prosdent.2020.12.017

Chouhan S, Sharma K, Guleria S (2017) Antimicrobial activity of some essential oils—Present status and future perspectives. Medicines 4(3): 58. https://doi.org/10.3390/medicines4030058

CLSI (2012) Clinical Laboratory Standard Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard-Ninth Edition. CLSI document M07-A9. Wayne, Pennsylvania: Clinical and Laboratory Standards Institute.

Creff-Froger C, Bessiral M, Fourmond M, Hédou C, Perrin-Guyomard A (2017) Certificates of analysis: A challenge to interpret. Euroreference 3: 27–34. http://doi.org/10.5281/zenodo.1172022

de Lucena-Ferreira SC, Cavalcanti IMG, Del Bel Cury AA (2013) Efficacy of denture cleansers in reducing microbial counts from removable partial dentures: A short-term clinical evaluation. Braz Dent J 24(4): 353–356. http://doi.org/10.1590/0103-6440201302183

De Vijlder T, Valkenborg D, Lemière F, Romijn EP, Laukens K, Cuyckens F (2018) A tutorial in small molecule identification via electrospray ionization-mass spectrometry: The practical art of structural elucidation. Mass Spectrom Rev 37(5): 607–629. http://doi.org/10.1002/mas.21551

Fujiwara N, Murakami K, Yoshida K, Sakurai S, Kudo Y, Ozaki K, Hirota K, Fujii H, Suzuki M, Miyake Y, Yumoto H (2020) Suppressive effects of 2-methacryloyloxyethyl phosphorylcholine (MPC)-polymer on the adherence of Candida species and MRSA to acrylic denture resin. Heliyon 6(6): e04211. http://doi.org/10.1016/j.heliyon.2020.e04211

Gunasena MT, Rafi A, Zobir SAM, Hussein MZ, Ali A, Kutawa AB, Wahab MAA, Sulaiman MR, Adzmi F, Ahmad K (2022) Phytochemicals profiling, antimicrobial activity and mechanism of action of essential oil extracted from ginger (Zingiber officinale Roscoe cv. Bentong) against Burkholderia glumae causative agent of bacterial panicle blight disease of rice. Plants 11(11): 1466. https://doi.org/10.3390/plants11111466

Günther E, Kommerein N, Hahnel S (2020) Biofilms on polymeric materials for the fabrication of removable dentures. Dtsch Zahnarztl Z Int 2(4): 142–151. http://dx.doi.org/10.3238/dzz-int.2020.0142-0151

Hayran Y, Sarikaya I, Aydin A, Tekin YH (2018) Determination of the effective anticandidal concentration of denture cleanser tablets on some denture base resins. J Appl Oral Sci 26: e20170077. https://doi.org/10.1590/1678-7757-2017-0077

Hermana Neppelenbroek K, Pavarina AC, Carlos Eduardo Vergani CE, Giampaolo ET(2005) Hardness of heat-polymerized acrylic resins after disinfection and long-term water immersion. J Prosthet Dent 93(2): 171–176. https://doi.org/10.1016/j.prosdent.2004.10.020

Hussein KA, Joo JH (2018) Antifungal activity and chemical composition of ginger essential oil against ginseng pathogenic fungi. Curr Res Environ Appl Mycol 8(2): 194–203. https://doi.org/10.5943/cream/8/2/4

Inayat A, Hassan SZ, Aziz A, Ibrahim R (2019) Denture induced stomatitis-A case report. Int J Endorsing Health Sci Res 7(4): 183–187. https://doi.org/10.29052/IJEHSR.v7.i4.2019.183-187

Islam M, Amin R, Ahmed M, Khatun S, Rahman ML, Siddiqui SA, Rahman M, Zahan M, Mannan M (2018) In-vitro antimicrobial activity of essential oils and different organic extracts of Lippia alba. J Phytochem Biochem 2(1): 107.

Jeffrey J, Satari MH, Kurnia D, Sudigdoadi S (2020) Inhibition of Streptococcus mutans growth induced by the extract of Citrus aurantifolia peel. J Int Dent Med Res 13(1): 122–127.

Jeffrey, Khaerunnisa R, Arifianti I, Azhari NK (2023) Antibacterial effect of telang flower (Clitoria ternatea) extract in eradicating Streptococcus mutans UA 159 biofilm mass. J Int Dent Med Res 16(2): 628–634.

Kati FA (2021) Effects of chemical disinfectants on surface hardness of heat-cured acrylic resins. In vitro study. J Oral Res 10(6): 1–6. https://doi.org/10.17126/joralres.2021.074

Kaypetch R, Anuwongnukroh N, Dechkunakorn S, Wichai W, Tua-ngam P, Tantivitayakul P, Shrestha B (2023) Novel vinegar solution for denture-cleansing agent. J Oral Sci 65(2): 117–120. https://doi.org/10.2334/josnusd.22-0385

Korenblum E, Goulart FRV, Rodrigues IA, Abreu F, Lins U, Alves PB, Blank AF, Valoni E, Sebastián GV, Alviano DS, Alviano CS, Seldin L (2013) Antimicrobial action and anti-corrosion effect against sulfate reducing bacteria by lemongrass (Cymbopogon citratus) essential oil and its major component, the citral. AMB Expr 3: 44. https://doi.org/10.1186/2191-0855-3-44

Koseki Y, Tanaka R, Murata H (2018) Development of antibacterial denture cleaner for brushing containing tea tree and lemongrass essential oils. Dent Mater J 37(4): 659–666. https://doi.org/10.4012/dmj.2017-295

Le Bars P, Kouadio AA, Bandiaky ON, Le Guéhennec L, de La Cochetière MF (2022) Host’s immunity and Candida species associated with denture stomatitis: A narrative review. Microorganisms 10: 1437. https://doi.org/10.3390/microorganisms10071437

Lee B, Yoon S, Lee JW, Kim Y, Chang J, Yun J, Ro JC, Lee JS, Lee JH (2020) Statistical characterization of the morphologies of nanoparticles through machine learning based electron microscopy image analysis. ACS Nano 14(12): 17125–17133. https://doi.org/10.1021/acsnano.0c06809

Li X, Robinson SM, Gupta A, Saha K, Jiang Z, Moyano DF, Sahar A, Riley MA, Rotello VM (2014) Functional gold nanoparticles as potent antimicrobial agents against multi-drug-resistant bacteria. ACS Nano 8(10): 10682–10686. https://doi.org/10.1021/nn5042625

Lira A, Consani R, Mesquita M, de Paula A (2014) Surface hardness of acrylic resins exposed to toothbrushing, chemical disinfection and thermocycling. J Res Pract Dent 2014: 466073. https://doi.org/10.5171/2014.466073

Manvitha K, Bidya B (2014) Review on pharmacological activity of Cymbopogon citratus. Int J Herbal Med 1(6): 5–7.

Mohialdeen H, Alnori A, Taqa A (2014) The effect of newly prepared cleansing agent on the hardness of highly impact acrylic denture base material. Al-Rafidain Dent J 14(1): 19–31. https://doi.org/10.33899/rden.2014.89249

Monteiro DR, de Souza Batista VE, Caldeirão ACM, Jacinto RC, Pessan JP (2021) Oral prosthetic microbiology: Aspects related to the oral microbiome, surface properties, and strategies for controlling biofilms. Biofouling 37(4): 353–371. https://doi.org/10.1080/08927014.2021.1912741

Nitu S, Patidar KC (2017) Evaluation of antimicrobial activity determination and phytochemical investigation in selected plants. Int J Pharmacogn Phytochem Res 9(12): 1429–1434.

O’Donnell LE, Robertson D, Nile CJ, Cross LJ, Riggio M, Sherriff A, Bradshaw D, Lambert M, Malcolm J, Buijs MJ, Zaura E, Crielaard W, Brandt BW, Ramage G (2015) The oral microbiome of denture wearers is influenced by levels of natural dentition. PLoS One 10(9): e0137717. https://doi.org/10.1371/journal.pone.0137717

Oktavia IN, Sutoyo S (2021) Article review: Synthesis of silver nanoparticles using bioreductor from plant extract as an antioxidant. UNESA J Chem 10(1): 37–54. https://doi.org/10.26740/ujc.v10n1.p37-54

Ozdal M, Gurkok S (2022) Recent advances in nanoparticles as antibacterial agent. ADMET 10: 115–129. https://doi.org/10.5599/admet.1172

Pereira CJ, Genari B, Leitune VCB, Collares FM, Samuel SMW (2019) Effect of immersion in various disinfectant solutions on the properties of a heat-cured acrylic resin. Rev Gaúcha Odontol 67: e20190052. https://doi.org/10.1590/1981-86372019000523090

Porwal A, Khandelwal M, Punia V, Sharma V (2017) Effect of denture cleansers on color stability, surface roughness, and hardness of different denture base resins. J Indian Prosthodont Soc 17(1): 61–67. https://doi.org/10.4103/0972-4052.197940

Rakib-Uz-Zaman SM, Hoque Apu E, Muntasir MN, Mowna SA, Khanom MG, Jahan SS, Akter N, Khan MAR, Shuborna NS; Shams SM, Khan K (2022) Biosynthesis of silver nanoparticles from Cymbopogon citratus leaf extract and evaluation of their antimicrobial properties. Challenges 13(1): 18. https://doi.org/10.3390/challe13010018

Raszewski Z, Nowakowska D, Więckiewicz W, Nowakowska-Toporowska A (2021) The effect of chlorhexidine disinfectant gels with anti-discoloration systems on color and mechanical properties of PMMA resin for dental applications. Polymers 13(11): 1800. https://doi.org/10.3390/polym13111800

Ribeiro SM, Fratucelli ÉDO, Bueno PCP, de Castro MKV, Francisco AA, Cavalheiro AJ, Klein MI (2019) Antimicrobial and antibiofilm activities of Casearia sylvestris extracts from distinct Brazilian biomes against Streptococcus mutans and Candida albicans. BMC Complement Altern Med 19(1): 308. https://doi.org/10.1186/s12906-019-2717-z

Rice SB, Chan C, Brown SC, Eschbach P, Han L, Ensor DS, Stefaniak AB, Bonevich J, Vladár AE, Hight Walker AR, Zheng J, Starnes C, Stromberg A, Ye J, Grulke EA (2013) Particle size distributions by transmission electron microscopy: An interlaboratory comparison case study. Metrologia 50(6): 663–678. https://doi.org/10.1088/0026-1394/50/6/663

Riyanto, Mulwandari M, Asysyafiiyah L, Sirajuddin MI, Cahyandaru N (2022) Direct synthesis of lemongrass (Cymbopogon citratus L.) essential oil-silver nanoparticles (EO-AgNPs) as biopesticides and application for lichen inhibition on stones. Heliyon 8(6): e09701. https://doi.org/10.1016/j.heliyon.2022.e09701

Rocha MM, Carvalho AM, Coimbra FCT, de Arruda CNF, Oliveira VC, Macedo AP, Silva-Lovato CH, Pagnano VA, Paranhos HO (2021) Complete denture hygiene solutions: Antibiofilm activity and effects on physical and mechanical properties of acrylic resin. J Appl Oral Sci 29: e20200948. https://doi.org/10.1590/1678-7757-2020-0948

Sahal G, Woerdenbag HJ, Hinrichs WLJ, Visser A, Tepper PG, Quax WJ, Mei HC, Bilkay IS (2020) Antifungal and biofilm inhibitory effect of Cymbopogon citratus (lemongrass) essential oil on biofilm forming by Candida tropicalis isolates; an in vitro study. J Ethnopharmacol 246: 112188. https://doi.org/10.1016/j.jep.2019.112188

Sahin C, Ergin A, Ayyildiz S, Cosgun E, Uzun G (2013) Effect of biofilm formation, and biocorrosion on denture base fractures. J Adv Prosthodont 5(2): 140–146. https://doi.org/10.4047/jap.2013.5.2.140

Sartawi SY, Abu-Hammad S, Salim NA, Al-Omoush S (2021) Denture stomatitis revisited: A summary of systematic reviews in the past decade and two case reports of papillary hyperplasia of unusual locations. Int J Dent 2021: 7338143. https://doi.org/10.1155/2021/7338143

Shariati A, Didehdar M, Razavi S, Heidary M, Soroush F, Chegini Z (2022) Natural compounds: A hopeful promise as an antibiofilm agent against Candida species. Front Pharmacol 13: 917787. https://doi.org/10.3389/fphar.2022.917787

Sharma N, Sheikh ZN, Alamri S, Singh B, Kesawat MS, Guleria S (2023) Chemical composition, antibacterial and combinatorial effects of the essential oils from Cymbopogon spp. and Mentha arvensis with conventional antibiotics. Agronomy 13(4): 1091. https://doi.org/10.3390/agronomy13041091

Sharmin S, Rahaman MM, Sarkar C, Atolani O, Islam MT, Adeyemi OS (2021) Nanoparticles as antimicrobial and antiviral agents: A literature-based perspective study. Heliyon 7(3): e06456. https://doi.org/10.1016/j.heliyon.2021.e06456

Smith DJ (2015) Characterization of Nanomaterials Using Transmission Electron Microscopy. In: Nanoscience & Nanotechnology. Nanocharacterisation. 2nd edn. Kirkland AI and Haigh SJ (eds.). The Royal Society of Chemistry, pp. 1–29. https://doi.org/10.1039/9781782621867-00001

Song X, Xia YX, He ZD, Zhang HJ (2018) A review of natural products with anti-biofilm activity. Curr Org Chem 22(8): 789–817. https://doi.org/10.2174/1385272821666170620110041

Souza TGF, Ciminelli VST, Mohallem NDS (2016) A comparison of TEM and DLS methods to characterize size distribution of ceramic nanoparticles. J Phys Conf Ser 733: 012039. https://doi.org/10.1088/1742-6596/733/1/012039

Sugiaman VK, Viando EJ, Pranata N (2023) Antibacterial activity of gedong manggo leaves Mangifera indica extract against Streptococcus mutans: Experimental study. J Ked Gi 35(2): 134–140. https://doi.org/10.24198/jkg.v35i2.46933

Sugiaman VK, Widowati W, Kusuma HSW, Salsabila N, Rizal R (2024) Antibacterial and antifungal properties of Citronella oil against Streptococcus mutans and Candida albicans by in vitro study. J Kedokteran Brawijaya 33(1): 1–5. https://doi.org/10.21776/ub.jkb.2024.033.01.1

Suwonchoochit T, Nagasevi N, Thamprechavai P, Aroonrerk N (2021) Efficiency of Hylocereus undatus extracts on biofilm formation of Streptococcus mutans in vitro. Int J Med Sci Curr Res 4(5): 834–840.

Tanhaeian A, Sekhavati MH, Moghaddam M (2020) Antimicrobial activity of some plant essential oils and an antimicrobial-peptide against some clinically isolated pathogens. Chem Biol Technol Agric 7: 13. https://doi.org/10.1186/s40538-020-00181-9

Tremi I, Havaki S, Georgitsopoulou S, Lagopati N, Gorgoulis VG, Georgakilas AG (2021) A guide for using transmission electron microscopy for studying the radiosensitizing effects of gold nanoparticles in vitro. Nanomaterials 11(4): 859. https://doi.org/10.3390/nano11040859

Volchkova IR, Yumashev AV, Borisov VV, Doroshina VI, Kristal EA, Repina SI (2020) Influence of removable denture cleaning agents on adhesion of oral pathogenic microflora in vitro: A randomized controlled trial. Open Dent J 14(1): 656–664. https://doi.org/10.2174/1874210602014010656

Wang L, Hu C, Shao L (2017) The antimicrobial activity of nanoparticles: Present situation and prospects for the future. Int J Nanomedicine 12: 1227–1249. https://doi.org/10.2147/IJN.S121956

Wen H, Luna-Romera JM, Riquelme JC, Dwyer C, Chang SLY (2021) Statistically representative metrology of nanoparticles via unsupervised machine learning of tem images. Nanomaterials 11(10): 2706. https://doi.org/10.3390/nano11102706

Yodmongkol S, Chantarachindawong R, Thaweboon S, Thaweboon B, Amornsakchai T, Srikhirin T (2014) The effects of silane-SiO2 nanocomposite films on Candida albicans adhesion and the surface and physical properties of acrylic resin denture base material. J Prosthet Dent 112(6): 1530–1538. https://doi.org/10.1016/j.prosdent.2014.06.019

© 2024 Journal of Pharmacy & Pharmacognosy Research

Anti-dormant mycobacterial of marine-derived fungi
J. Pharm. Pharmacogn. Res., vol. 13, no. 1, pp. 16-26, Jan-Feb 2025. DOI: https://doi.org/10.56499/jppres24.1953_13.1.16 Original Article Activity of ethyl acetate extracts of marine-derived fungi against active and hypoxia-induced dormant Mycobacterium [Actividad de extractos de acetato de etilo de hongos de origen marino contra Mycobacterium latente activa e inducida por hipoxia] Muhammad Azhari1, Atik Pereztia Litanjuasari1, … Continue reading Anti-dormant mycobacterial of marine-derived fungi
Rift Valley fever virus RdRp inhibition by RNA polymerase inhibitors
J. Pharm. Pharmacogn. Res., vol. 13, no. 1, pp. 1-15, Jan-Feb 2025. DOI: https://doi.org/10.56499/jppres24.1967_13.1.1 Original Article In silico study of RNA polymerase inhibitor drugs for Rift Valley fever virus using RdRp protein as the target [Estudio in silico de fármacos inhibidores de la ARN polimerasa para el virus de la fiebre del valle del Rift … Continue reading Rift Valley fever virus RdRp inhibition by RNA polymerase inhibitors
Probable interaction between levothyroxine and Thymus vulgaris
J. Pharm. Pharmacogn. Res., vol. 12, no. 6, pp. 1196-1198, Nov-Dec 2024. DOI: https://doi.org/10.56499/jppres24.2008_12.6.1196 Case Report Probable interaction between levothyroxine sodium and thyme (Thymus vulgaris), about a case report [Interacción probable entre levotiroxina sódica y tomillo (Thymus vulgaris), sobre un reporte de caso] Nassima Elyebdri1,2*, Sihem Baba Ahmed1, Nessrine Abourejal1, Lotfi Loudjedi3, Assia Bououden3, Nour … Continue reading Probable interaction between levothyroxine and Thymus vulgaris

© 2013-2020 by the authors; licensee JPPRes, Antofagasta, Chile. This journal is an open-access journal distributed under the terms and conditions of the Creative Commons Attribution license-Non Commercial 4.0 international. The content on this site is intended for health professionals. If you are not a health professional, please talk to your doctor about any doubts or concerns regarding your health

Made with ♥ by AVAGAX Studio