Metabolomic of propolis in hypertension

Excerpt:


J. Pharm. Pharmacogn. Res., vol. 12, no. 1, pp. 166-174, Jan-Feb 2024. DOI: https://doi.org/10.56499/jppres23.1705_12.1.166 Original Article Metabolomic study on the effect of Indonesian propolis in hypertensive rats [Estudio metabolómico del efecto del propóleo indonesio en ratas hipertensas] Ade Heri Mulyati1*, Henny Dwi Yanti1, Siti Warnasih1, Ahmad Sulaeman2, Mohamad Rafi3 1Department of Chemistry, Faculty of Mathematics … Continue reading Metabolomic of propolis in hypertension

J. Pharm. Pharmacogn. Res., vol. 12, no. 1, pp. 166-174, Jan-Feb 2024.

DOI: https://doi.org/10.56499/jppres23.1705_12.1.166

Original Article

Metabolomic study on the effect of Indonesian propolis in hypertensive rats

[Estudio metabolómico del efecto del propóleo indonesio en ratas hipertensas]

Ade Heri Mulyati1*, Henny Dwi Yanti1, Siti Warnasih1, Ahmad Sulaeman2, Mohamad Rafi3

1Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Pakuan, Bogor 16163, Indonesia.

2Department of Community Nutrition, Faculty of Human Ecology, Bogor Agricultural University, Bogor 16680, Indonesia.

3Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor 16680, Indonesia.

*E-mail: adeheri.mulyati@unpak.ac.id

Abstract

Context: Hypertension is a health problem that is a major factor triggering cardiovascular diseases; however, effective treatment has not been found.

Aims: To evaluate the effectiveness of Indonesian propolis in hypertension treatment through metabolomic study.

Methods: A total of 36 Sprague Dawley rats were divided into 6 groups, including standard diet group, high-NaCl diet group, high-NaCl diet + captopril group (25 mg/kg), high-NaCl diet + propolis from Riau Archipelago group, high-NaCl diet + propolis from Lampung group, high-NaCl diet + propolis from South Sulawesi group. An 8% NaCl diet was used to induce hypertension for 3 weeks, while 200 mg/ kg propolis was administered for 1 week following the induction. The bioactive compounds of ethanol extract of propolis were identified using UHPLC. Moreover, the metabolomic profile was performed using LC-MS/MS. Data was processed using principal component analysis and was used for metabolic pathway analysis.

Results: All propolis samples significantly ameliorated the blood pressure of hypertensive rats. The metabolites that were mainly found in rat serum were amino acids, glycerophospholipids, and acylcarnitine. Rats administered with propolis from South Sulawesi had the closest metabolite profile to the standard group, where L-acetylcarnitine could be the potential marker of hypertension. Several metabolic pathways, including the degradation of tryptophan, β-fatty acid oxidation, degradation of threonine, and 2-oxobutanoic acid, were the most impaired pathways in hypertension.

Conclusions: Indonesian propolis, especially from South Sulawesi, could be used for controlling blood pressure.

Keywords: biomarkers; blood pressure; hypertension; metabolomics; propolis.

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Resumen

Contexto: La hipertensión es un problema de salud que constituye un importante factor desencadenante de enfermedades cardiovasculares; sin embargo, no se ha encontrado un tratamiento eficaz.

Objetivos: Evaluar la eficacia del propóleo indonesio en el tratamiento de la hipertensión mediante un estudio metabolómico.

Métodos: Un total de 36 ratas Sprague Dawley fueron divididas en 6 grupos, incluyendo el grupo de dieta estándar, el grupo de dieta alta en NaCl, el grupo de dieta alta en NaCl + captopril (25 mg/kg), el grupo de dieta alta en NaCl + propóleos del archipiélago de Riau, el grupo de dieta alta en NaCl + propóleos de Lampung y el grupo de dieta alta en NaCl + propóleos de Sulawesi del Sur. Se utilizó una dieta con un 8% de NaCl para inducir la hipertensión durante 3 semanas, mientras que se administraron 200 mg/kg de propóleos durante 1 semana tras la inducción. Los compuestos bioactivos del extracto etanólico del propóleo se identificaron mediante UHPLC. Además, se realizó el perfil metabolómico mediante LC-MS/MS. Los datos se procesaron mediante análisis de componentes principales y se utilizaron para el análisis de rutas metabólicas.

Resultados: Todas las muestras de propóleos mejoraron significativamente la presión arterial de las ratas hipertensas. Los metabolitos que se encontraron principalmente en el suero de las ratas fueron aminoácidos, glicerofosfolípidos y acilcarnitina. Las ratas administradas con propóleos de Sulawesi meridional presentaron el perfil de metabolitos más parecido al del grupo estándar, en el que la L-acetilcarnitina podría ser el marcador potencial de la hipertensión. Varias vías metabólicas, como la degradación del triptófano, la oxidación de los ácidos β-grasos, la degradación de la treonina y el ácido 2-oxobutanoico, fueron las vías más alteradas en la hipertensión.

Conclusiones: El propóleo indonesio, especialmente el procedente de Sulawesi meridional, podría utilizarse para controlar la presión arterial.

Palabras Clave: biomarcadores; hipertensión; metabolómica; presión arterial; propóleos.

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Citation Format: Mulyati AH, Yanti HD, Warnasih S, Sulaeman A, Rafi M (2024) Metabolomic study on the effect of Indonesian propolis in hypertensive rats. J Pharm Pharmacogn Res 12(1): 166–174. https://doi.org/10.56499/jppres23.1705_12.1.166
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