OC-24: INHIBITION OF Helicobacter pylori BY Parthenium hysterophorus EXTRACTS

J Pharm Pharmacogn Res 3(suppl. 1): S43, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Oral Communication

OC-24: INHIBITION OF Helicobacter pylori BY Parthenium hysterophorus EXTRACTS

Espinosa-Rivero J1, Durán D1, Rendón-Huerta E2, Romero I1.

1Departamento de Bioquímica, 2Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, C.P. 04510, México, D.F., México. E-mail: jxyespinos@gmail.com, irma@bq.unam.mx


Introduction: Parthenium hysterophorus is a medicinal plant used to treat gastrointestinal disorders. Helicobacter pylori infects 50% of the world’s population and has been described as the etiological agent of gastritis, peptic ulcer, as well as gastric adenocarcinoma. The current therapy fails due to the antibiotic resistance; therefore, it is necessary to find new approaches to control H. pylori infection, either by its eradication or by preventing the bacterial colonization. This study investigates the effect of P. hysterophorus extracts on H. pylori growth and upon its colonization-related factors. Methods: Five different polarity extracts from roots and aerial parts were evaluated against H. pylori growth by the broth dilution method. Anti-colonization activities: motility in soft agar plates, urease activity by ammonia colorimetrical quantification, and adherence of FITC labeled H. pylori to AGS cells by fluorometrical measurement. Results: Organic extracts inhibited H. pylori growth. The dichloromethane extract from roots showed a MIC of 15.6 µg/mL while the aqueous extracts showed null activity.  There is a direct correlation between antibacterial activity and motility inhibition. Urease activity was partially inhibited by organic extracts, at best 46%, except for the roots dichloromethane extract, which reached 74% of inhibition with 500 µg/mL. Plant extracts inhibited adherence in different ranges but the dichloromethane-methanol ones possessed the highest effect (70% inhibition at 1 mg/mL). Conclusions: P. hysterophorus extracts have various biological activities that could act synergistically against H. pylori. This work contributes to the ethnomedical knowledge of this species and underlines the potential of some organic extracts as a good source for the isolation of bioactive compounds.


Citation Format: Espinosa-Rivero J, Durán D, Rendón-Huerta E, Romero I (2015) Inhibition of Helicobacter pylori by Parthenium hysterophorus extracts. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S43. Abstract nr OC-24.