J Pharm Pharmacogn Res 3(suppl. 1): S53, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
OC-35: EVALUATION OF OXIDATIVE STRESS EXPERIMENTAL MODEL AND TOXICITY OF THE SEED OIL EXTRACT Carapa guianensis AUBLET
Arencibia DF1, Narciandi J2, Rosario LA2, Vidal A3.
Introduction: The majoritarian presence of polyunsaturated fatty acids and phenolic compounds in the extract oil from the seed of Carapa guianensis Aublet could confer a powerful antioxidant effect. Methods: In this work the antioxidant effect of the extract on in vivo model of oxidative stress induction with cyclophosphamide (CF) in SD rats of both sexes administered orally for 21 days was evaluated. It was also evaluated the oral toxicity of the extract in acute study, mutagenesis and genotoxicity tests in vitro and in vivo. Results: The extract maintained the basal levels of antioxidant enzymes and markers of damage to biomolecules co-administered with CF (50 mg/kg intraperitoneally) at a dose of 400 mg/kg, also at this dose there was an imbalance in the water and food intake, body weight, hematology and blood chemistry. Non-systemic damages were observed when administered in a single dose of 2000 mg/kg in SD rats in classes’ method acute toxicity test. In the Ames test, the extract did not cytotoxic and mutagenic. In the in vitro test for chromosomal aberrations, it was not observed cells with increased chromosomal aberrations (structural and numerical) and not modified the mitotic index and number of polyploidy cells. On the comet assay of peripheral blood lymphocytes (SD rats), chromosomal aberrations, micronuclei and sperm head morphology assay performed in BALB/c mice did not genotoxic, but the dose of 2000 mg/kg administered orally for 35 days was cytotoxic in male germ cells. Conclusions: The preclinical results obtained indicate that this extract is antioxidant, also non-toxic systemically and is not mutagenic nor genotoxic according to the tests performed.
Citation Format: Arencibia DF, Narciandi J, Rosario LA, Vidal A (2015) Evaluation of oxidative stress experimental model and toxicity of the seed oil extract Carapa guianensis Aublet. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S53. Abstract nr OC-35.