J Pharm Pharmacogn Res 3(suppl. 1): S20, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
OC-59: SOME NEUROPHARMACOLOGICAL ACTIVITIES OF THE ETHANOLIC EXTRACT FROM Clusia minor L. LEAVES IN DIFFERENT CLASSICAL BEHAVIORAL MODELS IN MICE
Reynaldo G1, de Souza MM2, Malheiros A2, Dallas MT2, Aver K2, Piovesan LG2, Rodeiro I3, Bello A1, Mangas R1, Cechinel V2, Menéndez R3.
Introduction: Nowadays a number of herbal remedies are recommended as complementary medicine for psychiatric illness. Most of the potentialities of these remedies have been assured primarily in a variety of preclinical models. The phytochemical analysis of the ethanolic extract of Clusia minor L. leaves allowed the identification of various phytostosterols as well as triterpenoids and flavonoids. Since these compounds have presented several neuropharmacological actions, the present study was focused in evaluating the SNC effects of the extract by means of classical behavioral models in mice. Material and Methods: Single oral doses of the plant extract (100, 150 and 300 mg/kg) or vehicle and control drugs (n = 6-7 each) was administered 30 min prior to experiments. General locomotor activity was investigated by assessing open field and rota-rod performance. Anxiolytic and antidepressant effects were studied using Elevated Plus Maze (EPM) and forced swimming test (FST), respectively. Results: Neither locomotion nor motor coordination was altered by the oral administration of the extract. Results in EPM suggest no anxiolytic like effects. However, a decrease of the immobility time in FST (150 and 300 mg/kg) was observed. The results in EPM and FST are not simply the result of either a general modification of locomotor activity or of exploratory behavior consequent to exposure to a novel environment. Conclusions: The effect observed in the FST suggests an antidepressant like effects that may involve of catecholamine and/or serotonin transmission. However, other kinds of studies are obviously necessary to elucidate this mechanism of action. Our results also suggest that the extract does not act as a typical BDZ anxiolytic agent as the extract does not alter arm entries. Although the results agree with the phytochemical composition of the extract, further studies are necessary to confirm and extend these results.
Citation Format: Reynaldo G, de Souza MM, Malheiros A, Dallas MT, Aver K, Piovesan LG, Rodeiro I, Bello A, Mangas R, Cechinel V, Menéndez R (2015) Some neuropharmacological activities of the ethanolic extract from Clusia minor L. leaves in different classical behavioral models in mice. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S20. Abstract nr OC-59.