J Pharm Pharmacogn Res 3(suppl. 1): S70, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
OC-67: ANTIPROLIFERATIVE EFFECTS OF Sapindus saponaria L. ON PROSTATIC CANCER PC3 CELL LINE
Tamargo B1, Mena L1, Plaza LE2, Gómez Y3, Oliva BM3, Sierra G4.
Introduction: Sapindus saponaria L. presents important biological and pharmacological activities making them an object of study for the treatment of several diseases, including cancer. Among others, studies realized has been referring a good in vitro cytotoxic activity of S. saponaria L. against cancer cell lines Hela, WiDr and KB by other authors. Objectives: To detect and quantify the saponins present in the aqueous extracts of the seed, stem and fruit of S. saponaria L. and detect other phytoconstituents present in these extracts and evaluate the antiproliferative in vitro effect of these extracts on the cell line PC3 (human carcinoma of prostate) and to appreciate the toxicity of the same on blood’s erythrocytes. Methods: The Erythrocyte Hemolysis Assay was used to determine the concentration of saponins. In order to establish the presence of other possible components, phytochemical screening was performed. The effect of extracts from S. saponaria on PC 3 cell viability was determined by High Cell Density Assay. A spectrophotometric technique was used to determine the haemolytic potential of the extracts. Results: The extract of the pericarp had higher concentration of saponins, followed by the seeds and stem. Phytochemical analysis suggested the presence of saponins, tannins, reducing sugars and flavonoids in the three extracts. The three extracts showed a strong cytotoxic effect on cancer cell lines PC3. The fruit extract exhibited an inhibition percent of cell proliferation similar to paclitaxel on evaluated cancer cell line. The fruit extract showed a higher haemolytic potential than the seeds and stem extract, due to it contains higher concentration of saponins. Conclusions: The three extracts contained saponins and showed a good cytotoxic activity against PC3 cell line. S. saponaria is a valuable source of metabolites and could have a large relevance for alternative pharmacological therapy of prostate cancer and others.
Citation Format: Tamargo B, Mena L, Plaza LE, Gómez Y, Oliva BM, Sierra G (2015) Antiproliferative effects of Sapindus saponaria L. on prostatic cancer PC3 cell line. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S70. Abstract nr OC-67.