Pavetta crassipes reproductive studies

Excerpt:


J Pharm Pharmacogn Res 7(2): 135-143, 2019. Original Article | Artículo Original Reproductive parameters of male rats treated with Pavetta crassipes K.Schum. aqueous leaf extract [Parámetros reproductivos de ratas macho tratadas con extracto acuoso de hoja de Pavetta crassipes K.Schum.] Moses W. Bariweni1,*, Oboma I. Yibala2, Tolulokpe O. Alade2, Doubra F. Karibo1 1Department of Pharmacology and Toxicology, Faculty … Continue reading Pavetta crassipes reproductive studies

J Pharm Pharmacogn Res 7(2): 135-143, 2019.

Original Article | Artículo Original

Reproductive parameters of male rats treated with Pavetta crassipes K.Schum. aqueous leaf extract

[Parámetros reproductivos de ratas macho tratadas con extracto acuoso de hoja de Pavetta crassipes K.Schum.]

Moses W. Bariweni1,*, Oboma I. Yibala2, Tolulokpe O. Alade2, Doubra F. Karibo1

1Department of Pharmacology and Toxicology, Faculty of Pharmacy, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.
2Department of Medical Laboratory Science, Faculty of Basic Medical Science, College of Medicine, Niger Delta University, Wilberforce Island, Bayelsa State, Nigeria.

*E-mail: : mbariweni@yahoo.com, mbariweni@ndu.edu.ng

Abstract

Context: There is a growing concern about induction of male infertility resulting from medicinal plant use especially in malaria treatment. Carica papaya and Azadirachta indica are plants with documented antimalarial and anti-fertility effects. Pavetta crassipes leaves are reported to have antimalarial effects but there is no information about its reproductive toxicology.

Aim: To evaluate the effects of P. crassipes aqueous leaf extract on male reproductive parameters.

Methods: Healthy adult male rats were used for this study.  The extract was administered orally at 400, 800 and 1600 mg/kg for two and four weeks, respectively while the control group received 10 mL/kg distilled water. Blood samples were collected for serum hormonal assay. The testes and epididymis were excised for histological examination and seminal fluid analysis.

Results: Serum testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels reduced significantly in all treated groups compared to control but this effect was reversed after the respective recovery periods except FSH and LH levels in the group treated with 1600 mg/kg for four weeks. The control group had normal testicular histology while varying degrees of reversible histological changes occurred in the treated groups except persistent irregular basal lamina at 800 mg/kg in the two-week treated group while persistent irregular basal lamina and tubular atrophy occurred at 1600 mg/kg in the four-week treated group. Sperm count reduction at 800 and 1600 mg/kg compared to control (86 x 106 mL-1) in the groups treated for four weeks was irreversible.

Conclusions: The aqueous leaf extract of Pavetta crassipes has the potential to cause deleterious effects on male reproductive function in Wistar rats.

Keywords: hormone; infertility; reproduction; sperm-count; testosterone.

Resumen

Contexto: Existe una creciente preocupación por la inducción de infertilidad masculina resultante del uso de plantas medicinales, especialmente en el tratamiento de la malaria. Carica papaya y Azadirachta indica son plantas con efectos antipalúdicos y antifertilidad documentados. Se informa que las hojas de Pavetta crassipes tienen efectos antipalúdicos, pero no hay información sobre su toxicología reproductiva.

Objetivo: Evaluar los efectos del extracto acuoso de hoja de P. crassipes sobre los parámetros reproductivos masculinos.

Métodos: Se utilizaron ratas macho adultas sanas para este estudio. El extracto se administró por vía oral a 400, 800 y 1600 mg/kg durante dos y cuatro semanas, respectivamente, mientras que el grupo de control recibió 10 mL/kg de agua destilada. Se recogieron muestras de sangre para el análisis hormonal sérico. Los testículos y el epidídimo se extirparon para examen histológico y análisis de fluido seminal.

Resultados: La testosterona sérica, la hormona estimulante del folículo (FSH) y la hormona luteinizante (LH) se redujeron significativamente en todos los grupos tratados en comparación con el control, pero este efecto se revirtió después de los respectivos períodos de recuperación, excepto los niveles de FSH y LH en el grupo tratado con 1600 mg/kg para cuatro semanas. El grupo de control tenía histología testicular normal, mientras que en los grupos tratados se produjeron grados variables de cambios histológicos reversibles, excepto la lámina basal irregular persistente a 800 mg/kg en el grupo tratado de dos semanas, mientras que la lámina basal irregular persistente y la atrofia tubular ocurrieron a 1600 mg/kg en el grupo tratado de cuatro semanas. La reducción del recuento de espermatozoides a 800 y 1600 mg/kg en comparación con el control (86 x 106 mL-1) en los grupos tratados durante cuatro semanas fue irreversible.

Conclusiones: El extracto acuoso de la hoja de Pavetta crassipes tiene el potencial de causar efectos nocivos sobre la función reproductiva masculina en ratas Wistar.

Palabras Clave: hormona; esterilidad; recuento de esperma; reproducción; testosterona.

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Citation Format: Bariweni MW, Yibala OI, Alade TO, Karibo DF (2019) Reproductive parameters of male rats treated with Pavetta crassipes K.Schum. aqueous leaf extract. J Pharm Pharmacogn Res 7(2): 135–143.

© 2019 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

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