Phytochemistry and antibacterial activity of Crotalaria incana extracts

Excerpt:


J Pharm Pharmacogn Res 3(4): 100-108, 2015. DOI: https://doi.org/10.56499/jppres15.078_3.4.100 Original Article Phytochemical analysis and antibacterial activity on seed and pod extracts of Crotalaria incana [Análisis fitoquímico y actividad antibacteriana de extractos de semillas y vainas de Crotalaria incana] Mathewos Anza Alemu1*, Habtamu Gelaw Mekonnen1*, Milkyas Endale Annisa2 1Department of Chemistry, College of Natural and Computational Sciences, Debre … Continue reading Phytochemistry and antibacterial activity of Crotalaria incana extracts

J Pharm Pharmacogn Res 3(4): 100-108, 2015.

DOI: https://doi.org/10.56499/jppres15.078_3.4.100

Original Article

Phytochemical analysis and antibacterial activity on seed and pod extracts of Crotalaria incana

[Análisis fitoquímico y actividad antibacteriana de extractos de semillas y vainas de Crotalaria incana]

Mathewos Anza Alemu1*, Habtamu Gelaw Mekonnen1*, Milkyas Endale Annisa2

1Department of Chemistry, College of Natural and Computational Sciences, Debre Tabor University, P. O. Box 272, Debre Tabor, Ethiopia.
2Department of Chemistry, College of Natural and Computational Sciences, Hawassa University, P. O. Box 05, Hawassa, Ethiopia.
Abstract

Context: Crotalaria incana L. (Fabaceae) is used for various traditional medicinal treatments such as astringent, jaundice and palpitation, inflammation, skin disease and purgative.

Aims: To investigate the phytochemical constituents and evaluate the antibacterial activity of seed and pod extracts of C. incana.

Methods: Phytochemical screening tests were conducted to identify the class of compounds present in the dichloromethane/methanol (1:1) extracts of seed and pod. Silica gel column chromatographic technique was applied to separate the constituents of the extracts. Various spectroscopic techniques (IR, NMR (1H, 13C and DEPT-135) were applied to determine the structures of isolated compounds. Antibacterial activities were evaluated via disc diffusion method.

Results: Preliminary phytochemical screening of seed and pod extracts of C. incana L. revealed the presence alkaloids, flavonoids, triterpenes, tannins and steroids and absence of anthraquinones. Silica gel column chromatography separation of the extract yielded a known steroid β-sitosterol (1) and 1-nonadecanol (2). Evaluation of antibacterial activity of crude extract via disc diffusion method against Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia and Salmonella typhi showed moderate susceptibility of this selected Gram-positive and Gram-negative bacteria strains, comparable to the activity of the antibiotic (gentamicin).

Conclusions: The compound (2) (1-nonadecanol) was isolated for the first time from the C. incana. These results proved that polar extracts of the seeds and pods of C. incana possess antibacterial activity.

Keywords: Antibacterial; Crotalaria; Fabaceae; 1-nonadecanol; steroid.

Resumen

Contexto: Crotalaria incana L. (Fabaceae) es usada en varios tratamien-tos medicinales tradicionales como astringente, ictericia y palpitaciones, inflamación, enfermedades de la piel y purgante.

Objetivos: Investigar los constituyentes fitoquímicos y evaluar la actividad antibacteriana de extractos de semillas y vainas de C. incana.

Métodos: Se llevaron a cabo pruebas de tamizaje fitoquímico para identificar la clase de compuestos presentes en extractos de semilla y vaina en diclorometano/metanol (1:1). La técnica cromatográfica en columna de gel de sílice se aplicó para separar los constituyentes de los extractos. Diversas técnicas espectroscópicas (IR, RMN (1H, 13C y DEPT-135) se aplicaron para determinar las estructuras de los compuestos aislados. Las actividades antibacterianas fueron evaluadas mediante el método de difusión en disco.

Resultados: El tamizaje fitoquímico preliminar de los extractos de semillas y vainas de C. incana L. reveló la presencia de alcaloides, flavonoides, triterpenos, taninos y esteroides y ausencia de antraquinonas. Mediante la separación por cromatografía en columna en gel de sílice del extracto se aisló un conocido esteroide β-sitosterol (1) y 1-nonadecanol (2). La evaluación de la actividad antibacteriana de extracto crudo mediante el método de difusión en disco frente a Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae y Salmonella typhi mostraron susceptibilidad moderada a estas cepas de bacterias Gram-positivas y Gram-negativas seleccionadas, comparable a la actividad del antibiótico (gentamicina).

Conclusiones: El compuesto (2) (1-nonadecanol) fue aislado por primera vez de C. incana. Estos resultados demostraron que los extractos polares de las semillas y las vainas de C. incana poseen actividad antibacteriana.

Palabras Clave: Antibacteriana; Crotalaria; esteroide; Fabaceae; 1-nonadecanol.

Download the PDF file .

Citation Format: Mathewos Anza Alemu, Habtamu Gelaw Mekonnen, Milkyas Endale Annisa (2015) Phytochemical analysis and antibacterial activity on seed and pod extracts ofCrotalaria incana. J Pharm Pharmacogn Res 3(4): 100-108. DOI: https://doi.org/10.56499/jppres15.078_3.4.100

© 2015 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

Periodontal disease in people with HIV infection
J. Pharm. Pharmacogn. Res., vol. 13, no. 1, pp. 27-45, Jan-Feb 2025. DOI: https://doi.org/10.56499/jppres24.1941_13.1.27 Review Research trends in periodontal disease in people with HIV infection: A bibliometric analysis [Tendencias de investigación en enfermedad periodontal en personas con infección por VIH: Un análisis bibliométrico] Roberto Ojeda-Gómez1*, Julio C. Romero-Gamboa1, Melissa Pineda-Vega1, Pablo A. Millones-Gómez1, John E. … Continue reading Periodontal disease in people with HIV infection
Anti-dormant mycobacterial of marine-derived fungi
J. Pharm. Pharmacogn. Res., vol. 13, no. 1, pp. 16-26, Jan-Feb 2025. DOI: https://doi.org/10.56499/jppres24.1953_13.1.16 Original Article Activity of ethyl acetate extracts of marine-derived fungi against active and hypoxia-induced dormant Mycobacterium [Actividad de extractos de acetato de etilo de hongos de origen marino contra Mycobacterium latente activa e inducida por hipoxia] Muhammad Azhari1, Atik Pereztia Litanjuasari1, … Continue reading Anti-dormant mycobacterial of marine-derived fungi
Rift Valley fever virus RdRp inhibition by RNA polymerase inhibitors
J. Pharm. Pharmacogn. Res., vol. 13, no. 1, pp. 1-15, Jan-Feb 2025. DOI: https://doi.org/10.56499/jppres24.1967_13.1.1 Original Article In silico study of RNA polymerase inhibitor drugs for Rift Valley fever virus using RdRp protein as the target [Estudio in silico de fármacos inhibidores de la ARN polimerasa para el virus de la fiebre del valle del Rift … Continue reading Rift Valley fever virus RdRp inhibition by RNA polymerase inhibitors

© 2013-2020 by the authors; licensee JPPRes, Antofagasta, Chile. This journal is an open-access journal distributed under the terms and conditions of the Creative Commons Attribution license-Non Commercial 4.0 international. The content on this site is intended for health professionals. If you are not a health professional, please talk to your doctor about any doubts or concerns regarding your health

Made with ♥ by AVAGAX Studio