Phytochemistry and tyrosinase inhibition of Salvia officinalis extracts

J. Pharm. Pharmacogn. Res., vol. 10, no. 4, pp. 605-615, July-August 2022.

Original Article

Phytochemical characterization and mushroom tyrosinase inhibition of different extracts from Salvia officinalis L. leaves

[Caracterización fitoquímica e inhibición de la tirosinasa de hongos de diferentes extractos de hojas de Salvia officinalis L.]

Lana Y.M. Juee

Department of Pharmacognosy, Pharmacy College, Hawler Medical University, Erbil, Kurdistan Region, Iraq.

*E-mail: lana.juee@hmu.edu.krd

Abstract

Context: Sage (Salvia officinalis) is an ancient valuable plant used in the treatment of variant health issues.

Aims: To evaluate the depigmentation activity of S. officinalis leaf chloroformic (SOCF) and ethanolic (SOMF) extracts via its efficacy to inhibit tyrosinase enzyme using in vitro model and bioassay-guided identification and quantification of the main active constituents.

Methods: Plant extracts efficacy as a depigmentation agent has been studied via mushroom tyrosinase inhibition using in vitro model at two concentrations (100 and 200 µg/mL). Extracts were analyzed for phenolic compounds that could be responsible for the biological activity using LC-MS/MS analysis.

Results: Significant potency at a high concentration of 200 µg/mL for the methanolic extract were recorded (p≤0.05). The LC-MS/MS analysis of S. officinalis leaf extracts revealed the presence of eight and fourteen analytes of origin of thirty-seven analytes in both SOCF and SOMF, respectively. Analytes’ quantification recorded the highest amount for rosmarinic acid (46 016 µg/g) in SOMF and the lowest was hesperidin (0.6 µg/g) in SOCF.

Conclusions: S. officinalis extracts recorded significant tyrosinase inhibition potency could control the melanin synthesis process and exhibit beneficiary effect in hyperpigmentation issues.

Keywords: hesperidin; hyperpigmentation; LC-MS/MS spectroscopy; rosmarinic acid; sage; tyrosinase inhibitors.

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Resumen

Contexto: La salvia (Salvia officinalis) es una planta antigua y valiosa utilizada en el tratamiento de problemas de salud variantes.

Objetivos: Evaluar la actividad despigmentante de los extractos chorofórmico (SOCF) y etanólico (SOMF) de hojas de S. officinalis a través de su eficacia para inhibir la enzima tirosinasa utilizando un modelo in vitro y la identificación y cuantificación guiada por bioensayos de los principales componentes activos.

Métodos: La eficacia de los extractos de plantas como agente despigmentante se ha estudiado mediante la inhibición de la tirosinasa de hongos utilizando un modelo in vitro a dos concentraciones (100 y 200 µg/mL). Los extractos se analizaron en busca de compuestos fenólicos que pudieran ser responsables de la actividad biológica mediante análisis LC-MS/MS.

Resultados: Se registró potencia significativa a alta concentración 200 µg/mL para el extracto metanólico (p≤0.05). El análisis LC-MS/MS de extractos de hojas de salvia reveló la presencia de ocho y catorce analitos de origen de treinta y siete analitos tanto en SOCF como en SOMF, respectivamente. La cuantificación de los analitos registró la mayor cantidad de ácido rosmarínico (46 016 µg/g) en SOMF y la menor de hesperidina (0,6 µg/g) en SOCF.

Conclusiones: Los extractos de S. officinalis registraron una potencia significativa de inhibición de la tirosinasa que podría controlar el proceso de síntesis de melanina y exhibir un efecto beneficioso en los problemas de hiperpigmentación.

Palabras Clave: ácido rosmarínico; espectroscopia LC-MS/MS; hesperidina; hiperpigmentación; inhibidores de tirosinasa; salvia.

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Citation Format: Juee LYM (2022) Phytochemical characterization and mushroom tyrosinase inhibition of different extracts from Salvia officinalis L. leaves. J Pharm Pharmacogn Res 10(4): 605–615.
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