J Pharm Pharmacogn Res 3(suppl. 1): S164, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
PMO-71: ANTIOXIDANT EFFECTS FROM THE SEAWEED Sargassum SPP. EXTRACT IN UVB-RADIATED MURINE SKIN: POTENTIAL DERMOPROTECTIVE ACTION
Morales R1, Rodríguez M1, Menéndez R1, García T1, González K1, Hernández Y1, Valdés O1, Fernández M1, Nuñez R1, Cáceres L2, Govin L2.
Introduction: Ultraviolet (UV) radiation is a potent initiator of photooxidative mechanisms exerting detrimental effects on human skin by inducing oxidative stress responses mediated by reactive oxygen species. Thus, one approach to protecting humans from the harmful effects of UV irradiation is to use antioxidant substances, which can be added to preparations for topical application. The aim of this research was to determine in vivo antioxidant capacity of the seaweed Sargassum spp extract on mice exposed to acute UVB irradiation. Material and Methods: Male albino mice (Balb⁄C, 22-24g) were used. Prior to the assay, mice were depilated, irradiated for an interval of 6 min and treated with the dose 500, 250 y 125 µg of extract per cm² of skin. One side was shielded from irradiation to be used as control. The effects of Sargassum spp extract on markers of skin oxidative stress namely reduced glutathione (GSH) and superoxide dismutase (SOD) activity was studied. In parallel a group of treated and non-treated animals were observed to detect macroscopic dermal alterations. Results: Biochemical assays revealed a significant enhancement in the levels of skin GSH and SOD at doses 250 and 500 µg of extract per cm². Results of dermal alteration were consistent with favorable modification of oxidative stress parameters. Conclusions: Thus, our results suggest that the extract may contribute to skin repair by attenuating oxidative stress due to its in vivo antioxidant activity. However, further studies are now in progress in order to confirm these results by mean of histological assessment.
Citation Format: Morales R, Rodríguez M, Menéndez R, García T, González K, Hernández Y, Valdés O, Fernández M, Núñez R, Cáceres L, Govin L (2015) Antioxidant effects from the seaweed Sargassum spp. extract in UVB-radiated murine skin: potential dermoprotective action. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S164. Abstract nr PMO-71.