J Pharm Pharmacogn Res 3(suppl. 1): S121, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
PNA-29: EVALUATION OF ANTIOXIDANT ACTIVITY AND ACUTE TOXICITY OF AQUEOUS FRACTION OF Dichrostachys cinerea (L.) WIGHT & ARN. (Mimosaceae) LEAVES
Ribalta V1, Torres L2, Hernández E1, Sueiro M1, Armas Y1, Ruz V1.
Introduction: The aim of this study was to evaluate the in vitro antioxidant properties and the acute oral toxicity of the aqueous fraction of the D. cinerea leaves (AFDC) in Sprague-Dawley rats. Material and Methods: Plant material: Leaves were collected in the Botanical Garden of the university. Plant sample was identified as Dichrostachys cinerea by a taxonomic expert of the above institution. Phytochemical procedure: Dry powder (30 g) was extracted with methanol using Soxhlet apparatus. Methanol was removed under reduced pressure at 40°C to obtain a residue. It was suspended in water and partitioned with n-hexane to degrease. AFDC was collected and store at 2-8 °C until use. Antioxidant properties of D. cinerea: The ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl dihydrazyl (DPPH) and Fe2+-chelating assays were used. The total phenolic (TPC) and total flavonoids contents (TFC) were estimated by Folin-Ciocalteu and AlCl3 methods respectively, using galic acid (GA) and quercetin (Q) as standards. Acute Oral Toxicity: Test was carried out according to the single doses method on Sprague-Dawley rats at 2000 mg/kg body weight. Results and Discussion: Positive results were found for flavonoids, tannins and saponins. Antioxidant activity: AFDC had effective antioxidant properties giving IC50 values of 5.83 ± 0.03 µg/mL by ferric ions (Fe3+)- reducing power, 3.00 ± 0.00 µg/mL by DPPH assay and 8.28 ± 1.04 µg/mL by ferrous ions (Fe 2+) chelating activity. The total phenolic content (TPC) and total flavonoids content (TFC) were 243 ± 8.71 µg GAE/mg and 20 ± 0.48 µg QE/mg, respectively. Acute Toxicity: There was no mortality or behavioral abnormality in rats treated orally with a dose of 2000 mg/kg body weight. Conclusions: The results obtained suggest that the aqueous fraction of D. cinerea leaves possess significant in vitro antioxidant activity and can be considered as safe up to 2000 mg/kg body weight.
Citation Format: Ribalta V, Torres L, Hernández E, Sueiro M, Armas Y, Ruz V (2015) Evaluation of antioxidant activity and acute toxicity of aqueous fraction of Dichrostachys cinerea (L.) Wight & Arn. (Mimosaceae) leaves. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S121. Abstract nr PNA-29.