PNAI-35: ANTIMICROBIAL ACTIVITY OF THE TOTAL EXTRACT, FRACTIONS AND PURE COMPOUNDS OF THE LEAVES OF Excoecaria lucida SW.

FAPRONATURA, Natural Products, Pharmacology

J Pharm Pharmacogn Res 3(suppl. 1): S127, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.

Poster

PNAI-35: ANTIMICROBIAL ACTIVITY OF THE TOTAL EXTRACT, FRACTIONS AND PURE COMPOUNDS OF THE LEAVES OF Excoecaria lucida SW.

Gross C1, Munyewu B2, Ochoa A2, Escalona JC2, Camacho M3, Fechine J4, Sobral M4.

1Instituto Superior de Ciencias Médicas, Facultad 1, Avenida de las Américas, Santiago de Cuba.
2Departamento de Farmacia, Facultad de Ciencias Naturales, Universidad de Oriente. Patricio Lumumba s/n, Santiago de Cuba, Cuba. E-mail: aochoa@cnt.uo.edu.cu
3Centro de Estudios de Biotecnología Industrial, Facultad de Ciencias Naturales, Universidad de Oriente. Patricio Lumumba s/n, Santiago de Cuba, Cuba.
4Laboratorio de Tecnología Farmacéutica, Post-graduación en Productos Naturales y Sintéticos Bioactivos, Instituto de Ciencias de la Salud, Universidad Federal de Paraíba, Brasil.

 

Introduction: The Cuban population utilizes Excoecaria lucida Sw. (Aité) (Euphorbiaceae) for medicinal purposes (antimicrobial, antiasthmatic, toothache, callus destruction); however, the available scientific information is insufficient. Considering this background a study of its phytochemical and pharmacologic potential to support its terapeutic use is required. Methods: Seven samples obtained from the leaves of this plant species were  tested for the antimicrobial  activity [Total extract (10 and 100 mg/mL); ethyl acetate and butanolic fraction (5 and 100 mg/mL, respectively); Ellagic acid (1 and 20 mg/mL); 3,3´,4´-Tri-O-methylellagic acid; 3,3´,4´-Tri-O-methylellagic-4-O-B-D-glucopyranoside and Corilagin (1 mg/mL)]. The diffusion in disc method (Kirby-Bauer) was used for the six bacteria (Staphylococcus aureus; Escherichia coli; Enterococcus faecalis; Pseudomonas aeruginosa; Bacillus cereus; Bacillus megaterium), four fungi (Aspergillus niger; Alternaria alternata; Fusarium solani; Aspergillus versicolor) and a yeast (Candida albicans). The Minimum Concentration Inhibitory (MCI) and Minimum Concentration Bactericide (MCB) were determined using the broth dilution method. Results: The positive results were for the Total extract (100 mg/mL, sample 1) and ethyl acetate fraction (100 mg/mL, sample 2) that presented activity against Staphylococcus aureus, Enterococcus faecalis, Bacillus cereus and Bacillus megaterium, also butanolic fraction (100 mg/mL) had activity against Bacillus cereus and Bacillus megaterium. The sample had no antifungal activity. Conclusions: The total extract and ethyl acetate fraction had a large spectrum of antibacterial action as compared to the butanolic fraction. Ethyl acetate fraction demonstrated more antibacterial activity against the bacteria tested. The MCI and MCB were determined for the samples 1 and 2, with this confirming the highest antibacterial activity of the ethyl acetate fraction.

Citation Format: Gross C, Munyewu B, Ochoa A, Escalona JC, Camacho M, Fechine J, Sobral M (2015) Antimicrobial activity of the total extract, fractions and pure compounds of the leaves of Excoecaria lucida Sw. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S127. Abstract nr PNAI-35.

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