J Pharm Pharmacogn Res 3(suppl. 1): S103, 2015

Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015  September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.



Hernández-Barreto E, Pérez-Donato JA, Ribalta V, Torres LA, Sueiro M, Armas Y.

Pharmacy Department, Chemistry and Pharmacy Faculty. Central University of Las Villas. Road to Camajuaní km 5½. Santa Clara, Villa Clara, Cuba. Phone numbers: 042-211825(26); 042-227944. E-mail: enoelh@uclv.edu.cu


Introduction: The objective of this study was to determinate the possible voltage-operated-calcium channels (VOCCs) antagonism of Kigelia pinnata flowers extracts. Methods: Plant material: Fresh flowers of K. pinnata were collected in the Botanical Garden of the Central University of Las Villas. Plant sample was identified as Kigelia pinnata DC. (Bignoniaceae) by Orestes R. Méndez a taxonomic expert of above Institution. Phytochemical procedure: Etanolic (EF), Ethylacetate (EAF) and butanolic (BF) fractions from flowers were obtained by following method: 30 g of dry powder were subject to extraction in soxhlet apparatus with EtOH (90%w/v) for 6 hours. The initial ethanolic extract was re-suspended in bi-distilled water and subject to sequential liquid-liquid extraction procedure with n-hexane (discard), ethylacetate and n-butanol in order to obtain the respective fraction. The organic solvents were removed under vacuum at 40°C and solids residues were kept at -4°C for further studies. Isolated tissue studies: Aortic rings (5 mm) from Wistar rats 250-300 g were suspended in 10 mL organ bath filled with Krebs-Henseleit solution (pH=7.4), 37°C and continuously aerated with carbogen gas. Two grams of basal tension and equilibrate period of 1 h were applied before any drug exposure. KCl (30 mM) was used for elicited contraction and to check the tissue viability. KCl (50 mM) was used for VOCCs activation and after K. pinnata fractions were added (62 – 2000 µg/mL) in cumulative manner. Verapamil (0.01-10 µM) was used as reference. Statistics: Values are expressed as means ± S.D. One-way Anova test was used for statistical analysis, with p < 0.05 considered significant. Results: The relaxant potency order was: EAF, BF and EF. A direct relation with Phenols and Flavonoids contents was observed. EAF at 1000 µg/mL decreased contractile response about 50%. Conclusions: These results reveal at first time the vasorelaxant properties of K. pinnata flowers.


Citation Format: Hernández-Barreto E, Pérez-Donato JA, Ribalta V, Torres LA, Sueiro M, Armas Y (2015) Effects of Kigelia pinnata flowers extracts on voltage-operated-calcium channels. [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S103. Abstract nr PPP-11.