J Pharm Pharmacogn Res 3(suppl. 1): S136, 2015
Proceedings of the 4th International Symposium on Pharmacology of Natural Products FAPRONATURA 2015 September 21st-25th, 2015; Cuban Society of Pharmacology. Topes de Collantes, Sancti Spiritus, Cuba.
PTS-44: ACUTE ORAL TOXICITY AND ANTIINFLAMMATORY ACTIVITY OF THE ETHANOLIC EXTRACT AND FRACTIONS OF LEAVES OF Eugenia clarensis (BRITTON & P.WILSON)
Nguyen N1, Vicet-Muro L1, Siverio-Mota D1, Jorge-Rodríguez ME1, Coba-Sánchez Y1, González-Mosquera D1, Montenegro N2.
Introduction: Eugenia clarensis (Britton & P.Wilson) is an endangered plant endemic to Villa Clara, Cuba due to lack of knowledge of its uses. Objective: This study was aimed at evaluating acute toxicity and anti-inflammatory activity of the ethanol extract of leaves of E. clarensis and their fractions. Methods: The dried powder of leaves of E. clarensis was exhaustively extracted with ethanol and the resulting crude ethanol extract (CEE) was successively fractionated with n-hexane (HF), chloroform (CHF) and ethyl acetate (EAF) and lastly aqueous (AQF) fraction remained. Acute oral toxicity study was performed following OECD test guidelines 420 that the CEE was administered at 2000mg/kg b.wt. p.o and animals were observed for toxic signs at 0, 0.5, 1, 4, 24 h and for the next 14 days. The anti-inflammatory effects of CEE and their fractions were investigated in the model of acute 12-O-tetradecanoylphorbol-13-acetate (TPA) induced ear edema in mouse. Results: No adverse effects were observed in the acute CEE toxicity study. All the products showed strong anti-inflammatory activities in relation to the standard. The initial screening with CEE proved that this extract significantly inhibited (p≤0.05) ear edema in mice by 88.84%. The fractions of CEE were evaluated and showed that inhibition effect of CHF (96.53%), HF (95.36%) and EAF (91.93%) were statistically comparable with the indomethacin (97.65%). Acute study reveals that the LD50 of CEE is greater than 2000 mg/kg, b.wt. in female rats and can be classified as Category 5. Conclusions: Our results suggest that CHF, HF and EAF of leaves of Eugenia clarensis (Britton & P.Wilson) possess significant anti-inflammatory properties related to the presence of phenolic compounds (condensed tannins, flavonoids), coumarins y terpenes. Leaves of E. clarensis may therefore be considered as a good source for natural antiinflammatories and a possible pharmaceutical drug for the treatment of inflammation and related disorders.
Citation Format: Nguyen N, Vicet-Muro L, Siverio-Mota D, Jorge-Rodríguez ME, Coba-Sánchez Y, González-Mosquera D, Montenegro N (2015) Acute oral toxicity and antiinflammatory activity of the ethanolic extract and fractions of leaves of Eugenia clarensis (Britton & P.Wilson). [Abstract]. In: Proceedings of the FAPRONATURA 2015; 2015 Sep 21-25; Topes de Collantes, Sancti Spiritus: CSF. J Pharm Pharmacogn Res 3(Suppl. 1): S136. Abstract nr PTS-44.