Quercus ilex and Pinus halepensis biological effects

Excerpt:


J Pharm Pharmacogn Res 7(4): 260-272, 2019. Original Article | Artículo Original Phytochemical analysis, and antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex L. and Pinus halepensis Mill. methanolic extracts [Análisis fitoquímico y efectos antioxidante, antihemolítico y genoprotector de extractos metanólicos de Quercus ilex L. y Pinus halepensis Mill.] Hicham Meziti1*, Hamama Bouriche1, Seoussen Kada1, Ibrahim Demirtas2, Murat Kizil3, … Continue reading Quercus ilex and Pinus halepensis biological effects

J Pharm Pharmacogn Res 7(4): 260-272, 2019.

Original Article | Artículo Original

Phytochemical analysis, and antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex L. and Pinus halepensis Mill. methanolic extracts

[Análisis fitoquímico y efectos antioxidante, antihemolítico y genoprotector de extractos metanólicos de Quercus ilex L. y Pinus halepensis Mill.]

Hicham Meziti1*, Hamama Bouriche1, Seoussen Kada1, Ibrahim Demirtas2, Murat Kizil3, Abderrahmane Senator1

1Laboratory of Applied Biochemistry, Faculty of Natural Sciences and Life, University Ferhat Abbas, Sétif 1, Algeria.
2Laboratory of Plant Research, Department of Chemistry, Faculty of Science, Uluyazi Campus, Cankiri Karatekin University, Cankiri, Turkey.
3Department of Chemistry, Faculty of Science and Arts, University of Dicle, Diyarbakır, Turkey.

*E-mail: hicham.meziti@univ-setif.dz, hicham.meziti@yahoo.com

Abstract

Context: Quercus ilex and Pinus halepensis are largely used in Algerian folk medicine as a remedy for different health problems.

Aims: To determine the phytochemical composition as well as to evaluate the antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex root bark extract (QIE) and Pinus halepensis young ovulate cones extract (PHE).

Methods: Methanolic extracts were prepared by maceration. Phenolic compounds and flavonoids were quantified spectrophotometrically and identified by HPLC analysis. In vitro antioxidant activity of the extracts was assessed by determining the DPPH and ABTS radicals scavenging activities and ferric reducing/antioxidant power (FRAP). Protective effect of the extracts against AAPH-induced erythrocyte hemolysis was assessed. pBluescript M13 (+) plasmid DNA was used as oxidation target to evaluate DNA protective effect of the extracts.

Results: Considerable phenolic compounds and flavonoids contents were found in the studied extracts. Catechin and phenolic acids (4-hydoxybenzoic, caffeic, coumaric, ferulic and gentisic acids) were identified in QIE, while catechin and cinnamic acid were identified in PHE. Both extracts scavenge DPPH radical with IC50 values of 5.67 µg/mL and 18.87 µg/mL, respectively. Antioxidant capacity against ABTS radical was 4.13 and 1.15 mM Trolox equivalent/mg extract. The two extracts also showed a considerable ferric reducing ability with 1.98 and 8.55 mM FeSO4/mg extract, respectively. On the other hand, both extracts exerted a significant protective effect against AAPH-induced erythrocyte hemolysis and DNA protective activities were noticed.

Conclusions: Quercus ilex and Pinus halepensis extracts are important sources of bioactive compounds possessing important antioxidants, antihemolytic and genoprotective effects.

Keywords: anti-hemolytic; antioxidant; genoprotection; Pinus halepensis; Quercus ilex.

Resumen

Contexto: Quercus ilex y Pinus halepensis se usan en la medicina popular argelina como un remedio para diferentes problemas de salud.

Objetivos: Determinar la composición fitoquímica, así como evaluar los efectos antioxidantes, antihemolíticos y genoprotectores del extracto de corteza de raíz de Quercus ilex (QIE) y el extracto de conos ovulares de Pinus halepensis (PHE).

Métodos: Los extractos metanólicos se prepararon por maceración. Los compuestos fenólicos y los flavonoides se cuantificaron espectro-fotométricamente y se identificaron mediante análisis de HPLC. La actividad antioxidante in vitro se evaluó mediante la determinación de las actividades de captación de radicales DPPH y ABTS y la potencia férrica reductora/antioxidante (FRAP). Se evaluó el efecto protector de los extractos contra la hemólisis eritrocítica inducida por AAPH. El ADN plasmídico pBluescript M13 (+) se usó como diana de oxidación para evaluar el efecto protector de los extractos al ADN.

Resultados: En los extractos estudiados se encontraron considerables contenidos de compuestos fenólicos y de flavonoides. La catequina y los ácidos fenólicos (4-hidroxibenzoico, cafeico, cumárico, ferúlico y gentísico) se identificaron en QIE, mientras que la catequina y el ácido cinámico se identificaron en PHE. Ambos extractos eliminaron el radical DPPH con valores de CI50 de 5,67 µg/mL y 18,87 µg/mL, respectivamente. La capacidad antioxidante contra el radical ABTS fue 4,13 y 1,15 mM de equivalente de Trolox/mg de extracto. Los dos extractos también mostraron una considerable capacidad de reducción férrica con 1,98 y 8,55 mM FeSO4/mg, respectivamente. Por otro lado, ambos extractos ejercieron un efecto protector significativo contra la hemólisis eritrocítica inducida por AAPH y se observaron actividades protectoras al ADN.

Conclusiones: Los extractos de Quercus ilex y Pinus halepensis son fuentes importantes de compuestos bioactivos que poseen efectos antioxidantes, antihemolíticos y genoprotectores.

Palabras Clave: antihemolítico; antioxidante; genoprotección; Pinus halepensis; Quercus ilex.

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Citation Format: Meziti H, Bouriche H, Kada S, Demirtas I, Kizil M, Senator A (2019) Phytochemical analysis, and antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex L. and Pinus halepensis Mill. methanolic extracts. J Pharm Pharmacogn Res 7(4): 260–272.

© 2019 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

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