Rhomyrtus tomentosa and fibroblast growth factor expression

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J. Pharm. Pharmacogn. Res., vol. 12, no. 4, pp. 800-813, Jul-Aug 2024. DOI: https://doi.org/10.56499/jppres23.1810_12.4.800 Original Article Role of Rhodomyrtus tomentosa (Aiton) Hassk. in regulating the expression of fibroblast growth factor family in the liver of rat in a breast cancer model [Papel de Rhodomyrtus tomentosa (Aiton) Hassk. en la regulación de la expresión de la … Continue reading Rhomyrtus tomentosa and fibroblast growth factor expression

J. Pharm. Pharmacogn. Res., vol. 12, no. 4, pp. 800-813, Jul-Aug 2024.

DOI: https://doi.org/10.56499/jppres23.1810_12.4.800

Original Article

Role of Rhodomyrtus tomentosa (Aiton) Hassk. in regulating the expression of fibroblast growth factor family in the liver of rat in a breast cancer model

[Papel de Rhodomyrtus tomentosa (Aiton) Hassk. en la regulación de la expresión de la familia del factor de crecimiento de fibroblastos en el hígado de rata en un modelo de cáncer de mama]

Putri Cahaya Situmorang1*, Syafruddin Ilyas1, Rony Abdi Syahpura2, Reka Mustika Sari3, Alexander Patera Nugraha4, Alek Ibrahim5, Cheryl Grace Pratiwi Rumahorbo1

1Study Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia.

2Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

3Research Center for Food Technology and Processing, National Research and Innovation Agency (BRIN), Yogyakarta, Indonesia.

4Department Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.

5Research Center for Animal Husbandry, National Research and Innovation Agency (BRIN), West Java-Bogor, Indonesia.

*E-mail: putri.cahatya@usu.ac.id

Abstract

Context: Cellular abnormalities in the ducts and breast tissue cause breast cancer to invade nearby tissues like the liver. The fibroblast growth factor (FGF) signals are sent to its receptor and regulate various cellular processes to maintain liver homeostasis. However, this condition has few and inefficient treatments. Breast cancer is characterized by the development of cellular abnormalities within the ducts and breast tissue, leading to the invasion of adjacent tissues, such as the liver.

Aims: To examine the impact of Rhodomyrtus tomentosa administration on the expression of the FGF family in cancer animal models, with a specific focus on the liver.

Methods: Cancer model rats were administered at dosages of 100, 200, and 300 mg/kg BW for 30 days. Liver tissue and blood serum samples were extracted from the rat. Liver tissue was stained for immunohistochemistry using FGF1, FGF15, FGF19, and FGF21, and blood samples were collected for ELISA analysis.

Results: The study found that DMBA in hepatocyte cells degrades parenchyma, making it hydrophilic and necrosating. Hepatocyte cell function improved with the greatest dose of R. tomentosa. The elevation was associated with an area around the portal vein where recently dividing hepatocytes form clusters.

Conclusions: The promise of R. tomentosa as a candidate for the development of hepatoprotective medicines in cancer treatment arises from its ability to influence the expression of crucial liver health markers, including FGF1, FGF15, FGF19, and FGF21.

Keywords: fibroblast growth factor 1; fibroblast growth factor 15; fibroblast growth factor 19; fibroblast growth factor 21; Rhodomyrtus tomentosa.

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Resumen

Contexto: Las anomalías celulares en los conductos y el tejido mamario hacen que el cáncer de mama invada tejidos cercanos como el hígado. Las señales del factor de crecimiento de fibroblastos (FGF) se envían a su receptor y regulan diversos procesos celulares para mantener la homeostasis hepática. Sin embargo, esta enfermedad tiene pocos e ineficaces tratamientos. El cáncer de mama se caracteriza por el desarrollo de anomalías celulares dentro de los conductos y el tejido mamario, que conducen a la invasión de tejidos adyacentes, como el hígado.

Objetivos: Examinar el impacto de la administración de Rhodomyrtus tomentosa sobre la expresión de la familia del FGF en modelos animales de cáncer, con especial atención al hígado.

Métodos: Se administraron dosis de 100, 200 y 300 mg/kg de peso corporal a ratas modelo de cáncer durante 30 días. Se extrajeron muestras de tejido hepático y suero sanguíneo de la rata. El tejido hepático se tiñó para inmunohistoquímica con FGF1, FGF15, FGF19 y FGF21, y se recogieron muestras de sangre para análisis ELISA.

Resultados: El estudio encontró que el DMBA en células de hepatocitos degrada el parénquima, haciéndolo hidrofílico y necrosante. La función de las células hepatocitarias mejoró con la mayor dosis de R. tomentosa. La elevación se asoció con un área alrededor de la vena porta donde los hepatocitos en división reciente forman grupos.

Conclusiones: La promesa de R. tomentosa como candidato para el desarrollo de medicamentos hepatoprotectores en el tratamiento del cáncer surge de su capacidad para influir en la expresión de marcadores cruciales para la salud hepática, incluyendo FGF1, FGF15, FGF19 y FGF21.

Palabras Clave: factor de crecimiento de fibroblastos 1; factor de crecimiento de fibroblastos 15; factor de crecimiento de fibroblastos 19; factor de crecimiento de fibroblastos 21; Rhodomyrtus tomentosa.

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Citation Format: Situmorang PC, Ilyas S, Syahputra RA, Sari RM, Nugraha AP, Ibrahim A, Rumahorbo CGP (2024) Role of Rhodomyrtus tomentosa (Aiton) Hassk. in regulating the expression of fibroblast growth factor family in the liver of rat in a breast cancer model. J Pharm Pharmacogn Res 12(4): 800–813. https://doi.org/10.56499/jppres23.1810_12.4.800
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