J. Pharm. Pharmacogn. Res., vol. 14, no. 2, Article ID 2467, Mar-Apr 2026.

DOI: https://doi.org/10.56499/jppres_14.2.2467

Original article

Salacca zalacca peel modulates uricemia and IL-6 in rats

 

[La cáscara de Salacca zalacca modula la uricemia y la IL-6 en ratas]

 

Danis Pertiwi^1*, Dimar K. Puspaningrum¹, Fanny A. Mardhiani²

 

¹Department of Clinical Pathology, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Central Java, 50112, Indonesia.

²Department of Biology, Faculty of Science and Mathematics, Diponegoro University, Semarang, Central Java, 50275, Indonesia.

*E-mail: danispertiwi@unissula.ac.id

 

Abstract

Context: Hyperuricemia promotes oxidative stress and inflammation. Ethanolic salak (Salacca zalacca) peel extract (ESP) contains flavonoids with antioxidant potential.

Aims: To evaluate the effects of ESP on serum uric acid (UA), interleukin-6 (IL-6), and glutathione peroxidase (GPx) protein levels in hyperuricemic rats.

Methods: Male Wistar rats (n=30) were allocated to five groups (normal control; hyperuricemic control; allopurinol 9 mg/kg; ESP 210 or 420 mg/kg). Hyperuricemia was induced using a high-purine chicken-liver regimen. UA was measured pre- and post-treatment by a uricase-peroxidase colorimetric assay (520 nm). IL-6 and GPx protein levels were determined by enzyme-linked immunosorbent assay (ELISA) (450 nm).

Results: ESP 420 mg/kg reduced UA to 2.22 ± 0.06 mg/dL (Δ5.78 ± 0.10), lowered IL-6 to 32.96 ± 1.51 pg/mL, and increased GPx protein levels to 51.19 ± 0.72 pg/mL. Group differences were significant (ANOVA, p<0.001), with a dose-response trend for ESP.

Conclusions: ESP modulated uric acid, IL-6, and GPx in hyperuricemic rats, supporting its potential as a natural adjuvant for the management of hyperuricemia.

Keywords: glutathione peroxidase; hyperuricemia, interleukin-6; Salacca zalacca; uric acid.

 

 

Resumen

Contexto: La hiperuricemia promueve el estrés oxidativo y la inflamación. El extracto etanólico de cáscara de salak (Salacca zalacca) (ESP) contiene flavonoides con potencial antioxidante.

Objetivos: Evaluar los efectos del ESP sobre los niveles séricos de ácido úrico (AU), interleucina-6 (IL-6) y glutatión peroxidasa (GPx) en ratas hiperuricémicas.

Métodos: Se asignaron ratas Wistar macho (n = 30) a cinco grupos (control normal; control hiperuricémico; alopurinol 9 mg/kg; ESP 210 o 420 mg/kg). La hiperuricemia se indujo mediante una dieta rica en purinas a base de hígado de pollo. El AU se midió antes y después del tratamiento mediante un ensayo colorimétrico de uricasa-peroxidasa (520 nm). Los niveles de IL-6 y GPx se determinaron mediante ensayo inmunoenzimático (ELISA) (450 nm).

Resultados: ESP 420 mg/kg redujo el ácido úrico a 2,22 ± 0,06 mg/dL (Δ5,78 ± 0,10), disminuyó la IL-6 a 32,96 ± 1,51 pg/mL e incrementó los niveles de proteína GPx a 51,19 ± 0,72 pg/mL. Las diferencias entre grupos fueron significativas (ANOVA, p<0,001), observándose una relación dosis-respuesta para ESP.

Conclusiones: ESP moduló el ácido úrico, la IL-6 y la GPx en ratas hiperuricémicas, lo que respalda su potencial como coadyuvante natural para el tratamiento de la hiperuricemia.

Palabras Clave: ácido úrico; glutatión peroxidasa; hiperuricemia; interleucina-6; Salacca zalacca.

 

 

https://jppres.com/jppres/pdf/vol14/jppres_14.2.2467.pdf

 

 

Citation Format: Pertiwi D, Puspaningrum DK, Mardhiani FA (2026) Salacca zalacca peel modulates uricemia and IL-6 in rats. J Pharm Pharmacogn Res 14(2): 2467. https://doi.org/10.56499/jppres_14.2.2467

 

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