Sida spp. antioxidant and metabolite profiling

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J. Pharm. Pharmacogn. Res., vol. 11, no. 3, pp. 401-413, May-June 2023. DOI: https://doi.org/10.56499/jppres22.1551_11.3.401 Original Article Metabolite profiling, in vitro antioxidant and xanthine oxidase inhibitory properties of six Sumatran sidaguri (Sida L.) [Perfil de metabolitos, propiedades antioxidantes e inhibidoras de la xantina oxidasa in vitro de seis sidaguri (Sida L.) de Sumatra] Ema Ratna Sari1,2, Netty … Continue reading Sida spp. antioxidant and metabolite profiling

J. Pharm. Pharmacogn. Res., vol. 11, no. 3, pp. 401-413, May-June 2023.

DOI: https://doi.org/10.56499/jppres22.1551_11.3.401

Original Article

Metabolite profiling, in vitro antioxidant and xanthine oxidase inhibitory properties of six Sumatran sidaguri (Sida L.)

[Perfil de metabolitos, propiedades antioxidantes e inhibidoras de la xantina oxidasa in vitro de seis sidaguri (Sida L.) de Sumatra]

Ema Ratna Sari1,2, Netty Suharti1, Friardi Ismed1,3, Deddi Prima Putra1,3*

1Faculty of Pharmacy, Andalas University, Padang 25163, Indonesia.

2STIFI Bhakti Pertiwi Palembang, 30128, Indonesia.

3Laboratory of Biota Sumatera, Andalas University, Padang 25163, Indonesia.

*E-mail: deddiprima@phar.unand.ac.id

Abstract

Context: The Sida genus, part of the Malvaceae family, is commonly used in traditional medicine due to its many beneficial properties, such as antigout, anti-rheumatic, anti-inflammatory, antitumor, and hepatoprotective effects. Various species of Sida are found in Indonesia, particularly on the island of Sumatra, and may hold potential for future drug development.

Aims: To assess and compare the methanol leaf extracts of three Sida species (S. acuta, S. rhombifolia, and S. cordifolia) from West Sumatra and South Sumatra, Indonesia, for their chemical content in metabolite profiling, in vitro antioxidant capacity, and xanthine oxidase inhibitory activity.

Methods: The LC-MS/MS method was used to determine metabolite profiling, while spectrophotometry was used to assess total phenolic content, flavonoid content, antioxidant capacity, and xanthine oxidase inhibitor activity.

Results: The results showed that S. rhombifolia (WS) had the highest total phenolic (64.872 ± 1.525 mg GAE/g) and flavonoid contents (13.890 ± 0.816 mg QE/g), S. acuta (SS) exhibited the highest antioxidant activity (IC50 147.295 ± 3.364 µg/mL), and S. cordifolia (WS) showed the highest xanthine oxidase inhibitor activity (IC50 110.087 ± 15.637 µg/mL).

Conclusions: These findings suggest that S. acuta, S. rhombifolia, and S. cordifolia may be potential sources of polyphenols and antioxidants and could also serve as promising natural plant sources for developing antigout remedies.

Keywords: antioxidant capacity; metabolite profiling; Sida L.; sidaguri; xanthine oxidase inhibitor.

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Resumen

Contexto: El género Sida, perteneciente a la familia Malvaceae, se utiliza habitualmente en medicina tradicional por sus numerosas propiedades beneficiosas, como efectos antigotosos, antirreumáticos, antiinflamatorios, antitumorales y hepatoprotectores. En Indonesia, sobre todo en la isla de Sumatra, se encuentran varias especies de Sida que pueden tener potencial para el desarrollo futuro de fármacos.

Objetivos: Evaluar y comparar los extractos metanólicos de las hojas de tres especies de Sida (S. acuta, S. rhombifolia y S. cordifolia) de Sumatra occidental y Sumatra meridional, Indonesia, para determinar su contenido químico en perfiles de metabolitos, su capacidad antioxidante in vitro y su actividad inhibidora de la xantina oxidasa.

Métodos: El método LC-MS/MS se utilizó para determinar el perfil de metabolitos, mientras que la espectrofotometría se utilizó para evaluar el contenido fenólico total, el contenido de flavonoides, la capacidad antioxidante y la actividad inhibidora de la xantina oxidasa.

Resultados: Los resultados mostraron que S. rhombifolia (WS) tenía el mayor contenido fenólico total (64,872 ± 1,525 mg GAE/g) y de flavonoides (13,890 ± 0,816 mg QE/g), S. acuta (SS) exhibió la mayor actividad antioxidante (IC50 147,295 ± 3,364 µg/mL), y S. cordifolia (WS) mostró la mayor actividad inhibidora de la xantina oxidasa (IC50 110,087 ± 15,637 µg/mL).

Conclusiones: Estos resultados sugieren que S. acuta, S. rhombifolia y S. cordifolia pueden ser fuentes potenciales de polifenoles y antioxidantes, y también podrían servir como fuentes de plantas naturales prometedoras para desarrollar remedios contra la gota.

Palabras Clave: capacidad antioxidante; inhibidor de la xantina oxidasa; perfil de metabolitos; Sida L.; sidaguri.

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Citation Format: Sari ER, Suharti N, Ismed F, Putra DP (2023) Metabolite profiling, in vitro antioxidant and xanthine oxidase inhibitory properties of six Sumatran sidaguri (Sida L.). J Pharm Pharmacogn Res 11(3): 401–413. https://doi.org/10.56499/jppres22.1551_11.3.401
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