Spray-drying Perilla frutescens extract optimization

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J. Pharm. Pharmacogn. Res., vol. 12, no. 6, pp. 1056-1066, Nov-Dec 2024. DOI: https://doi.org/10.56499/jppres24.2009_12.6.1056 Original Article Optimization of spray-drying conditions and quality assessment of dry extract from Perilla frutescens (L.) Britton leaves [Optimización de las condiciones de secado por atomización y evaluación de la calidad del extracto seco de hojas de Perilla frutescens (L.) Britton] … Continue reading Spray-drying Perilla frutescens extract optimization

J. Pharm. Pharmacogn. Res., vol. 12, no. 6, pp. 1056-1066, Nov-Dec 2024.

DOI: https://doi.org/10.56499/jppres24.2009_12.6.1056

Original Article

Optimization of spray-drying conditions and quality assessment of dry extract from Perilla frutescens (L.) Britton leaves

[Optimización de las condiciones de secado por atomización y evaluación de la calidad del extracto seco de hojas de Perilla frutescens (L.) Britton]

Nguyen Thi Linh Tuyen1*, Nguyen Thi Thuy Linh1, Pham Thi To Lien1, Do Quang Duong2

1Department of Pharmacognosy, Faculty of Pharmacy, Can Tho University of Medicine and Pharmacy, 179 Nguyen Van Cu Street, Can Tho City, Vietnam.

2Department of Pharmaceutical Information Technology, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, 41 Dinh Tien Hoang Street, District 1, Ho Chi Minh City, Vietnam.

*E-mail: ntltuyen@ctump.edu.vn

Abstract

Context: Perilla frutescens and its dried extract have been employed in food and pharmaceutical manufacturing due to the plant's biological activities, which include antibacterial, anti-allergic, anticancer, antiviral, antioxidant, and antidepressant properties. Despite numerous studies on preparing dry extracts from various medicinal herbs, no research has been conducted to optimize spray-drying conditions specifically for P. frutescens.

Aims: To optimize the spray-drying parameters using BCPharSoft software and evaluate the quality of P. frutescens dry extract.

Methods: D-optimal design based on four independent variables carrier type (Glucidex, Glucidex: Arabic gum 9:1, Glucidex: Arabic gum 8:2), carrier mass (10, 15, and 20 g), inlet air temperature (160, and 180℃) and flow rate (12, and 14 rpm/min) was applied to investigate the cause-effect relations and optimized preparation process. The drying performance, moisture content, total phenol, and total flavonoid content were investigated as four dependent variables.

Results: All independent variables had significant effects on the dependent variables. The optimal parameters of the preparation process included using carrier Glucidex: Arabic gum at a ratio of 9:1, carrier mass of 19 g, inlet air temperature of 161°C, and flow rate of 12 rpm/min. The drying performance, moisture content, total phenol, and flavonoid content of the optimized P. frutescens dry extract were found to be 10.42%, 4.80%, and 90.57 mg GA/g, and 53.55 mg QE/g, respectively.

Conclusions: Dried P. frutescens extract has been efficiently produced using the spray-drying technique, establishing a foundation for future manufacturing of pharmaceuticals and functional foods derived from P. frutescens.

Keywords: BCPharSoft software; dry extract; Perilla frutescens; spray-drying.

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Resumen

Contexto: La Perilla frutescens y su extracto seco se han utilizado en la fabricación de alimentos y productos farmacéuticos debido a las actividades biológicas de la planta, que incluyen propiedades antibacterianas, antialérgicas, anticancerígenas, antivirales, antioxidantes y antidepresivas. A pesar de numerosos estudios sobre la preparación de extractos secos de varias hierbas medicinales, no se ha realizado ninguna investigación para optimizar las condiciones de secado por pulverización específicamente para la P. frutescens.

Objetivos: Optimizar los parámetros de secado por pulverización utilizando el software BCPharSoft y evaluar la calidad del extracto seco de P. frutescens.

Métodos: Se aplicó un diseño D-óptimo basado en cuatro variables independientes: tipo de portador (Glucidex, Glucidex: goma arábiga 9:1, Glucidex: goma arábiga 8:2), masa del portador (10, 15 y 20 g), temperatura del aire de entrada (160 y 180℃) y velocidad de flujo (12 y 14 rpm/min) para investigar las relaciones causa-efecto y optimizar el proceso de preparación. El rendimiento de secado, el contenido de humedad, el contenido total de fenoles y el contenido total de flavonoides fueron investigados como cuatro variables dependientes.

Resultados: Todas las variables independientes tuvieron efectos significativos en las variables dependientes. Los parámetros óptimos del proceso de preparación incluyeron el uso de un portador Glucidex: goma arábiga en una proporción de 9:1, una masa de portador de 19 g, una temperatura del aire de entrada de 161°C y una velocidad de flujo de 12 rpm/min. El rendimiento de secado, el contenido de humedad, el contenido total de fenoles y el contenido total de flavonoides del extracto seco de P. frutescens optimizado fueron de 10,42%; 4,80% y 90,57 mg de ácido gálico/g y 53,55 mg de equivalente de quercetina/g, respectivamente.

Conclusiones: El extracto seco de P. frutescens se ha producido de manera eficiente mediante la técnica de secado por aspersión, estableciendo una base para la futura fabricación de productos farmacéuticos y alimentos funcionales derivados de P. frutescens.

Palabras Clave: extracto seco; Perilla frutescens; secado por pulverización; software BCPharSoft.

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Citation Format: Tuyen NTL, Linh NTT, Lien PTT, Duong DQ (2024) Optimization of spray-drying conditions and quality assessment of dry extract from Perilla frutescens (L.) Britton leaves. J Pharm Pharmacogn Res 12(6): 1056–1066. https://doi.org/10.56499/jppres24.2009_12.6.1056
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