Tag Archives: antidiabetic activity

Antidiabetic potential of Ecobolium linneanum leaf extracts

J. Pharm. Pharmacogn. Res., vol. 10, no. 3, pp. 496-507, May-June 2022.

Original Article

Antidiabetic potential of leaf extracts of Ecobolium linneanum Kurz in streptozotocin-induced diabetic rats

[Potencial antidiabético de extractos de hojas de Ecobolium linneanum Kurz en ratas diabéticas inducidas por estreptozotocina]

Srilakshmi Nallapaty1, Narender Malothu1*, Rajeswari Pasupula2,Alekhya Ketha3

1Department of Pharmaceutical Chemistry, KL College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, AP, 522 502, India.

2Department of Pharmacology, KL College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, AP, 522 502, India.

3Department of Pharmaceutical Chemistry, AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, AP, India.

*E-mail: mnarender@kluniversity.in, narendermalothu@gmail.com

Abstract

Context: In a general context, the importance’s of several medicinal plants has been explored in treating diabetes mellitus. In this connection, the antidiabetic potentiality needs to be established for one of the medicinally emerging plants Ecbolium linneanum Kurz.

Aims: To evaluate the antidiabetic activity of petroleum ether, ethyl acetate, and methanol extracts of E. linneanum leaves in streptozotocin-induced diabetic rats.

Methods: Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg BW) in male Wistar rats. Diabetic rats were treated orally with petroleum ether, ethyl acetate and methanol extracts of E. linneanum leaves at two doses (100 and 200 mg/kg BW/day) for 14 days. The blood glucose, lipid profile, serum enzyme levels, glycosylated haemoglobin (HbA1c), creatinine and serum urea levels were monitored. Glibenclamide (10 mg/kg BW) was used as a standard hypoglycaemic drug.

Results: The methanolic leaf extract at 200 mg/kg BW showed increasingly substantial (p<0.001) antidiabetic activity on day 14 and also significantly reduced (p<0.001) SGOT, SGPT, TG, TC, VLDL, LDL, HbA1c and antioxidant enzymes (SOD, CAT and GSH levels) increased significantly (p<0.001) compared to diabetic group. Renal parameters also decreased significantly (p<0.001) at both doses relative to diabetic control, but petroleum leaf extract and ethyl acetate leaf extract exhibited a less significant effect (p<0.05) compared to the methanol extract. All the extracts of leaves showed a protective effect on the oxidative status of the liver at tested concentrations (100 and 200 mg/kg BW).

Conclusions: The present research findings revealed that E. linneanum leaf possesses significant anti-hyperglycaemic activity and encourages conventional use in the treatment of diabetes mellitus under the experimental conditions exposed in this study.

Keywords: antidiabetic activity; Ecbolium linneanum; oxidative status; phytochemical screening; streptozotocin.

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Resumen

Contexto: Se ha explorado la importancia de varias plantas medicinales en el tratamiento de la diabetes mellitus. A este respecto, es necesario establecer la potencialidad antidiabética de una de las plantas medicinales emergentes Ecbolium linneanum Kurz.

Objetivos: Evaluar la actividad antidiabética de extractos de éter de petróleo, acetato de etilo y metanol de hojas de E. linneanum en ratas diabéticas inducidas por estreptozotocina.

Métodos: Se indujo diabetes mediante inyección intraperitoneal de estreptozotocina (60 mg/kg de peso corporal) en ratas Wistar macho. Ratas diabéticas fueron tratadas por vía oral con extractos de éter de petróleo, acetato de etilo y metanol de hojas de E. linneanum en dos dosis (100 y 200 mg/kg PC/día) durante 14 días. Se monitorizaron los niveles de glucosa en sangre, perfil lipídico, enzimas séricas, hemoglobina glicosilada (HbA1c), creatinina y urea sérica. Se utilizó glibenclamida (10 mg/kg de peso corporal) como fármaco hipoglucemiante estándar.

Resultados: El extracto de hoja metanólico a 200 mg/kg de peso corporal mostró una actividad antidiabética cada vez más sustancial (p<0,001) en el día 14 y también redujo significativamente (p<0,001) SGOT, SGPT, TG, TC, VLDL, LDL, HbA1c y enzimas antioxidantes (niveles de SOD, CAT y GSH) aumentaron significativamente (p<0,001) en comparación con el grupo de diabéticos. Los parámetros renales también disminuyeron significativamente (p<0,001) en ambas dosis en relación con el control diabético, pero el extracto de hoja de petróleo y el extracto de hoja de acetato de etilo exhibieron un efecto menos significativo (p<0,05) en comparación con el extracto metanólico. Todos los extractos de hojas mostraron un efecto protector sobre el estado oxidativo del hígado a las concentraciones probadas (100 y 200 mg/kg de peso corporal).

Conclusiones: Los hallazgos de la presente investigación revelaron que la hoja de E. linneanum posee una importante actividad antihiperglucémica y fomenta el uso convencional en el tratamiento de la diabetes mellitus en las condiciones experimentales expuestas en este estudio.

Palabras Clave: actividad antidiabética; Ecbolium linneanum; estado oxidativo; estreptozotocina; tamizaje fitoquímico.

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Citation Format: Srilakshmi N, Narender M, Rajeswari P, Alekhya K (2022) Antidiabetic potential of leaf extracts of Ecobolium linneanum Kurz in streptozotocin-induced diabetic rats. J Pharm Pharmacogn Res 10(3): 496–507.
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© 2022 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

Calotropis procera antidiabetic activity according extraction method



J Pharm Pharmacogn Res 10(1): 30-38, 2022.

Original Article

Impact of extraction mode on phytoconstituents and antidiabetic activity of extracts of Calotropis procera (Aiton) Dryand.

[Impacto del modo de extracción en los fitoconstituyentes y la actividad antidiabética de los extractos de Calotropis procera (Aiton) Dryand.]

Sadaf Iftikhar1*, Khalid Hussain1, Muhammad Saleem2, Naureen Shehzadi1, Farhat Saghir1, Ayisha Shaukat1, Surriya Naheed 1, Sidra Siddique1

1Department of Pharmaceutical Chemistry, Punjab University College of Pharmacy, University of the Punjab, Lahore, Pakistan.

2 Department of Pharmacology, Punjab University College of Pharmacy, University of the Punjab, Lahore, Pakistan.

*E-mail: principal.pharmacy@pu.edu.pk, sadafahsan67@gmail.com

Abstract

Context: The extraction mode plays a key role in extracting a particular type of pharmacologically active compounds that can be isolated as leads to develop novel therapeutic molecules.

Aims: To evaluate the impact of extraction mode on extract yield, phytochemicals constituents, and antidiabetic activity of different parts of the Calotropis procera.

Methods: The different parts extracts were prepared by maceration and Soxhlet extraction. The resulting extracts were subjected to determining phytochemical constituents and antidiabetic activity using in vitro models (inhibition of alpha-amylase, glucose uptake by yeast cell, and inhibition of non-enzymatic Hb-glycation).

Result: The yield of extracts was higher in Soxhlet extraction in both solvents compared to maceration. Likewise, methanol extract of all the parts prepared by Soxhlet extraction exhibited higher contents of total polyphenols and tannins (430.2 ± 0.99 and 228.3 ± 3.8, respectively). On the other hand, total flavonoids and terpenoids were more in all parts prepared by maceration. The extract of flower and stem prepared by maceration exhibited 64.87 ± 0.720%, 70.32 ± 0.75%, and 56.86 ± 0.13% antidiabetic activity in alpha-amylase enzyme, non – enzymatic Hb glycation inhibition, and glucose enhancement in yeast, respectively (p<0.05) as compared to standards. The IC50 of alpha-amylase inhibitory assay and non-enzymatic Hb-glycation was 600 µg/mL and 420 µg/mL, respectively, and EC50 for glucose uptake assay was 400 µg/mL.

Conclusions: This study revealed that maceration extracts with higher flavonoid and terpenoid content have higher antidiabetic action than Soxhlet extracts. This study will assist in the future for the isolation of antidiabetic compounds from the plant.

Keywords: antidiabetic activity; Calotropis procera; maceration; phytochemical constituents; Soxhlet extraction.

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Resumen

Contexto: El modo de extracción juega un papel clave en la extracción de un tipo particular de compuestos farmacológicamente activos que pueden aislarse como conductos para desarrollar nuevas moléculas terapéuticas.

Objetivos: Evaluar el impacto del modo de extracción en el rendimiento del extracto, los componentes fitoquímicos y la actividad antidiabética de diferentes partes de Calotropis procera.

Métodos: Los extractos de las diferentes partes se prepararon mediante maceración y extracción Soxhlet. Los extractos resultantes se sometieron a la determinación de constituyentes fitoquímicos y actividad antidiabética utilizando modelos in vitro (inhibición de alfa amilasa, captación de glucosa por células de levadura e inhibición de glicación de Hb no enzimática).

Resultados: Se encontró que el rendimiento de extractos fue mayor en la extracción Soxhlet en ambos solventes en comparación con la maceración. Asimismo, el extracto metanólico de todas las partes elaborado por extracción Soxhlet presentó mayores contenidos de polifenoles totales y taninos (430.2 ± 0.99 and 228.3 ± 3.8, respectivamente). Por otro lado, los flavonoides totales y terpenoides fueron más en todas las partes, preparados por maceración. El extracto de flor y tallo preparado por maceración exhibió 64,87 ± 0,720%, 70,32 ± 0,75% y 56,86 ± 0,13% de actividad antidiabética en la enzima alfa amilasa, inhibición de la glucosilación de Hb no enzimática y mejora de la glucosa en levadura respectivamente (p<0,05) en comparación a los estándares.

Conclusiones: Este estudio reveló que los extractos de maceración con mayor contenido de flavonoides y terpenoides tienen mayor acción antidiabética que los extractos de Soxhlet. Este estudio ayudará en el futuro a aislar compuestos antidiabéticos de plantas.

Palabras Clave: actividad antidiabética; Calotropis procera; constituyentes fitoquímicos; extracción Soxhlet; maceración.

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Citation Format: Iftikhar S, Hussain K, Saleem M, Shehzadi N, Saghir F, Shaukat A, Naheed S, Siddique S (2022) Impact of extraction mode on phytoconstituents and antidiabetic activity of extracts of Calotropis procera (Aiton) Dryand. J Pharm Pharmacogn Res 10(1): 30–38.

© 2022 Journal of Pharmacy & Pharmacognosy Research (JPPRes)