Tag Archives: antinociception

Pharmacological studies of A. leiocarpa leaves

J. Pharm. Pharmacogn. Res., vol. 10, no. 3, pp. 459-468, May-June 2022.

Original Article

Pharmacological studies of anti-inflammatory, anti-nociceptive and anti-pyretic compounds found in chromatographic fractions of Anogeissus leiocarpa (DC). Guill. & Perr. leaves

[Estudios farmacológicos de compuestos antiinflamatorios, antinociceptivos y antipiréticos encontrados en fracciones cromatográficas de hojas de Anogeissus leiocarpa (DC). Guill. & Perr.]

Precious A. Idakwoji1*, Francis O. Atanu1, Paul C. Nweje-Anyalowu2,9, Theophilus B. Momoh3, Wisdom O. Oniwon1, Sara T. Elazab4, Souty M.Z. Sharkawi5,10, Rania M. Waheed6, Amal Youssef7, Gaber El-Saber Batiha8

1Department of Biochemistry, Faculty of Natural Science, Kogi State University, P.M.B. 1008, Anyigba, Kogi State, Nigeria.

2Biochemistry Programme, Department of Chemical Sciences, Faculty of Science, Clifford University, Owerrinta, Abia State, Nigeria.

3Department of Plant Science and Biotechnology, Faculty of Natural Science, Kogi State University, P.M.B. 1008, Anyigba, Kogi State, Nigeria.

4Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.

5Department of Pharmacology and Toxicology, Faculty of Pharmacy, Nahda University, Egypt.

6Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt.

7Medical Pharmacology Department, Faculty of Medicine, Cairo University, Egypt.

8Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Albeheira, Egypt.

9Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.

10Department of Pharmacology and Toxicology, Faculty of Pharmacy, Beni-Suef University, Egypt.

*E-mail: precious.ia@ksu.edu.ng


Context: The anti-nociceptive and anti-pyretic properties of the crude aqueous extract of Anogeissus leiocarpa (DC). Guill. & Perr. leaves have been reported.

Aims: To investigate the anti-inflammatory, anti-nociceptive, and anti-pyretic properties of aqueous extract of A. leiocarpa leaves (AEAL) and its fractions to identify the bioactive compound(s).

Methods: AEAL was fractionated successively and eluted in gradients of solvent mixture (n-hexane, ethylacetate, and methanol). Eluates, which showed similar TLC profiles were pooled to afford 5 fractions (F1-F5). AEAL and the fractions were subjected to phytochemical analysis and investigated for anti-inflammatory, anti-nociceptive, and anti-pyretic effects using carrageenan-induced paw edema and Brewer’s yeast-induced pyrexia in rats, and acetic acid-induced writhing in mouse models, respectively.

Results: Phytochemical analysis indicated presence of terpenoids, saponins, steroids, glycosides, flavonoids, tannins and alkaloids. At 200 mg/kg b.w, AEAL and F1-F5 recorded significant (p<0.05) improvements in paw edema, abdominal constrictions and pyrexia, which is comparable to the standard (ketoprofen or Aspirin), relative to the control groups, with F4 observed to be the most potent fraction. GC-MS analysis of F4 reveal the presence of hexadecenoic acid, 15-methyl-, methylester, 9-oxabicyclo (6.1.0) nonane, cis-, cholest-4-en-3-one, cholesterol and 3-diazo-1-methyl-1, 3-dihydro-indol-2-one.

Conclusions: The results indicate that identified compounds, particularly 3-dihydro-indol-2-one, could be responsible for the anti-inflammatory, anti-nociceptive, and anti-pyretic properties of the extract.

Keywords: Anogeissus leiocarpa; anti-inflammatory; antinociception; antipyretic; inflammation; NSAID; pyrexia.


Contexto: Se han reportado las propiedades anti-nociceptivas y antipiréticas del extracto acuoso crudo de hojas de Anogeissus leiocarpa (DC). Guill. & Perr.

Objetivos: Investigar las propiedades antiinflamatorias, anti-nociceptivas y antipiréticas del extracto acuoso de hojas de A. leiocarpa (AEAL) y sus fracciones para identificar los compuestos bioactivos.

Métodos: AEAL se fraccionó sucesivamente y se eluyó en gradientes de mezcla de solventes (n-hexano, acetato de etilo y metanol). Los eluatos que mostraron perfiles de TLC similares se agruparon para proporcionar 5 fracciones (F1-F5). AEAL y las fracciones se sometieron a análisis fitoquímicos y se investigaron los efectos antiinflamatorios, anti-nociceptivos y antipiréticos utilizando edema de la pata inducido por carragenina y pirexia inducida por levadura de Brewer en ratas, y retorcimiento inducido por ácido acético en modelos de ratón, respectivamente.

Resultados: El análisis fitoquímico indicó presencia de terpenoides, saponinas, esteroides, glucósidos, flavonoides, taninos y alcaloides. A 200 mg/kg p.c., AEAL y F1-F5 registraron mejoras significativas (p<0,05) en el edema de la pata, constricciones abdominales y pirexia que es comparable al estándar (ketoprofeno o Aspirina), en relación con los grupos de control, y se observó que F4 era la fracción más potente. El análisis GC-MS de F4 revela la presencia de ácido hexadecenoico, 15-metil-, éster metílico, 9-oxabiciclo (6.1.0) nonano, cis-, colest-4-en-3-ona, colesterol y 3-diazo-1 -metil-1,3-dihidro-indol-2-ona.

Conclusiones: Los resultados indican que los compuestos identificados, particularmente la 3-dihidro-indol-2-ona, podrían ser responsables de las propiedades anti-inflamatorias, anti-nociceptivas y anti-piréticas del extracto.

Palabras Clave: AINE; Anogeissus leiocarpa; anti-inflamatorio; anti-nocicepción; antipirético; inflamación; pirexia.

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Citation Format: Idakwoji PA, Atanu FO, Nweje-Anyalowu PC, Momoh TB, Oniwon WO, Elazab ST, Sharkawi SMZ, Waheed RM, Youssef A, Batiha GE-S (2022) Pharmacological studies of anti-inflammatory, anti-nociceptive and anti-pyretic compounds found in chromatographic fractions of Anogeissus leiocarpa (DC). Guill. & Perr. leaves. J Pharm Pharmacogn Res 10(3): 459–468.

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