Tag Archives: caffeic acid phenethyl ester

TNF-alpha and NF-kB expressions during OTM post-administration of CAPE

J. Pharm. Pharmacogn. Res., vol. 10, no. 6, pp. 1037-1045, November-December 2022.

DOI: https://doi.org/10.56499/jppres22.1479_10.6.1037

Original Article

Anti-inflammatory effect of caffeic acid phenethyl ester supplementation on TNF-α and NF-κB expressions throughout experimental tooth movement in vivo

[Efecto antiinflamatorio de la suplementación con éster fenetílico de ácido cafeico en las expresiones de TNF-α y NF-κB a través del movimiento dental experimental in vivo]

Kirana Salikha1, Ida Bagus Narmada1*, Alida1, Alexander Patera Nugraha1,2, Annisa Fitria Sari2, Wibi Riawan3, Tengku Natasha Eleena Binti Tengku Ahmad Noor4

1Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.

2Graduate Student of Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.

3Biomolecular Biochemistry, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia.

4Membership of Faculty of Dental Surgery, Royal College of Surgeon, Edinburgh University, United Kingdom.

*E-mail: ida-b-n@fkg.unair.ac.id


Context: Orthodontic tooth movement (OTM) changes the periodontal tissue and increases the incidence of root resorption (OIRR). Caffeic acid phenethyl ester (CAPE), an antioxidant and anti-inflammatory chemical generated from honey propolis, might be useful in controlling inflammation during OTM and so reducing the risk of OIRR.

Aims: To evaluate if CAPE supplementation has an anti-inflammatory impact on tumor necrosis factor-α (TNF-α) and nuclear transcription factor κB (NF-κB) during experimental OTM in male Wistar rats (Rattus novergicus).

Methods: Forty-eight healthy male Wistar rats were divided into positive control group (OTM 10 g/mm2 force application) and experimental group (OTM application and CAPE administration). Each groups were observed for 3, 7, 14 days. A nickel-titanium closed coil spring that was 8.0 mm long, thick was inserted between the upper left first molar and upper central incisor in order to move the molar mesially. A 20 mg/kg body weight dose of CAPE was taken orally. Using immunohistochemistry, the expression of TNF-α and NF-κB was examined on the compression side of the OTM. Both the Tukey’s honest significant difference test and the one-way analysis of variance test were applied (p<0.05).

Results: TNF-α and NF-κB expression in the compression side differed considerably across groups (p<0.05). Daily administration of CAPE significantly downregulates TNF-α and NF-κB expression on the compression side.

Conclusions: Administration of CAPE throughout OTM can successfully reduce the number of TNF-α and NF-κB expressions in the compression side in vivo.

Keywords: caffeic acid phenethyl ester; experimental tooth movement; medicine; nuclear transcription factor-κB; tumor necrosis factor-α.



Contexto: El movimiento dental ortodóncico (OTM) cambia el tejido periodontal y aumenta la incidencia de reabsorción radicular (OIRR). El éster fenetílico del ácido cafeico (CAPE), un químico antioxidante y antiinflamatorio generado a partir del propóleo de la miel, podría ser útil para controlar la inflamación durante la OTM y así reducir el riesgo de OIRR.

Objetivos: Evaluar si la suplementación con CAPE tiene un impacto antiinflamatorio sobre el factor de necrosis tumoral-α (TNF-α) y el factor de transcripción nuclear κB (NF-κB) durante OTM experimental en ratas Wistar macho (Rattus novergicus).

Métodos: Cuarenta y ocho ratas Wistar macho sanas se dividieron en un grupo de control positivo (aplicación de fuerza de 10 g/mm2 de OTM) y un grupo experimental (aplicación de OTM y administración de CAPE). Cada grupo se observó durante 3, 7, 14 días. Se insertó un resorte helicoidal cerrado de níquel-titanio de 8,0 mm de largo y espesor entre el primer molar superior izquierdo y el incisivo central superior para mover el molar mesialmente. Se tomó por vía oral una dosis de 20 mg/kg de peso corporal de CAPE. Usando inmunohistoquímica, se examinó la expresión de TNF-α y NF-κB en el lado de compresión del OTM. Se aplicaron tanto la prueba de diferencia significativa honesta de Tukey como la prueba de análisis de varianza de una vía (p<0,05).

Resultados: La expresión de TNF-α y NF-κB en el lado de compresión difirió considerablemente entre los grupos (p<0,05). La administración diaria de CAPE reguló significativamente a la baja la expresión de TNF-α y NF-κB en el lado de la compresión.

Conclusiones: La administración de CAPE a través de OTM puede reducir con éxito las expresiones de TNF-α y NF-κB en el lado de compresión in vivo.

Palabras Clave: éster fenetílico del ácido cafeico; factor de necrosis tumoral-α; factor de transcripción nuclear-κB; medicamento; movimiento dental experimental.

Citation Format: Salikha K, Narmada IB, Alida, Nugraha AP, Sari AF, Riawan W, Noor TNEBTA (2022) Anti-inflammatory effect of caffeic acid phenethyl ester supplementation on TNF-α and NF-κB expressions throughout experimental tooth movement in vivo. J Pharm Pharmacogn Res 10(6): 1037–1045. https://doi.org/10.56499/jppres22.1479_10.6.1037

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