Tag Archives: histopathology

Acute toxicity of interruptin-rich extract in rats

J. Pharm. Pharmacogn. Res., vol. 10, no. 5, pp. 800-811, September-October 2022.

DOI: https://doi.org/10.56499/jppres22.1392_10.5.800

Original Article

Acute oral toxicological evaluation in Wistar rats of interruptin-rich extract from Cyclosorus terminans and its in vitro antidiabetic potential

[Evaluación toxicológica oral aguda en ratas Wistar del extracto de Cyclosorus terminans rico en interruptina y su potencial antidiabético in vitro]

Sujinda Songtrai1,2, Kwanchanok Dejyong3, Sireewan Kaewsuwan1,2*

1Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, 90110, Thailand.

2Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Songkhla, 90110, Thailand.

3Laboratory Animal Facility Unit, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand.

*E-mail: songsri.k@psu.ac.th

Abstract

Context: Interruptins A and B derivatives from edible fern Cyclosorus terminans have been reported properties including anti-bacterial, anti-cancer, anti-oxidation, anti-inflammatory, and antidiabetic activities. Unfortunately, studies on its safety are still scarce.

Aims: To evaluate the acute oral toxicity of interruptin-rich extract (IRE) from C. terminans in Wistar rats and examine the antidiabetic potential of IRE in vitro.

Methods: IRE was evaluated cytotoxicity by MTT assay and potency of glucose consumption in hepatocyte and skeletal muscle cells. IRE was evaluated for acute toxicity in Wistar rats by following OECD 420 guidelines. Wistar rats were single oral administrated of 2000 mg/kg IRE and further observed for 14 days. LCMS was assessed for verifying IRE absorption into the bloodstream. Hematological, biochemical parameters and microscopic examination of heart, liver and kidney were evaluated.

Results: IRE demonstrated no cytotoxicity toward hepatocytes and skeletal muscle cells and facilitated glucose consumption into cells. In the acute toxicity study, on day 14, after a single oral administration of 2000 mg/kg IRE, no mortality and behavioral alterations. There was no change in metabolic parameters. Histopathology of heart, liver and kidney showed normal architecture.

Conclusions: Thus, LD50 of IRE was considered superior to 2000 mg/kg. Hence the extract can be utilized safely and could provide a capability for diabetic control.

Keywords: acute toxicity; antidiabetic; Cyclosorus terminans; histopathology; Wistar rats.

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Resumen

Contexto: Se han informado propiedades de los derivados de las interruptinas A y B del helecho comestible Cyclosorus terminans, que incluyen actividades antibacterianas, anticancerígenas, antioxidantes, antiinflamatorias y antidiabéticas. Desafortunadamente, los estudios sobre su seguridad aún son escasos.

Objetivos: Evaluar la toxicidad oral aguda del extracto rico en interruptina (IRE) de C. terminans en ratas Wistar y examinar el potencial antidiabético de IRE in vitro.

Métodos: Se evaluó la citotoxicidad de IRE mediante ensayo MTT y la potencia de consumo de glucosa en hepatocitos y células de músculo esquelético. Se evaluó la toxicidad aguda de IRE en ratas Wistar siguiendo las directrices de la OCDE 420. A ratas Wistar se les administró por vía oral una sola dosis de 2000 mg/kg de IRE y se observaron durante 14 días. Se evaluó mediante LCMS para verificar la absorción de IRE en el torrente sanguíneo. Se evaluaron parámetros hematológicos, bioquímicos y examen microscópico de corazón, hígado y riñón.

Resultados: IRE no demostró citotoxicidad hacia los hepatocitos y las células del músculo esquelético y facilitó el consumo de glucosa en las células. En el estudio de toxicidad aguda, el día 14, tras una única administración oral de 2000 mg/kg IRE, no hubo mortalidad ni alteraciones del comportamiento. No hubo cambios en los parámetros metabólicos. La histopatología de corazón, hígado y riñón mostró una arquitectura normal.

Conclusiones: Así, la DL50 de IRE se consideró superior a 2000 mg/kg. Por lo tanto, el extracto se puede utilizar de forma segura y proporcionaría una capacidad para el control de la diabetes.

Palabras Clave: toxicidad aguda; antidiabético; Cyclosorus terminans; histopatología; ratas Wistar.

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Citation Format: Songtrai S, Dejyong K, Kaewsuwan K (2022) Acute oral toxicological evaluation in Wistar rats of interruptin-rich extract from Cyclosorus terminans and its in vitro antidiabetic potential. J Pharm Pharmacogn Res 10(5): 800–811. https://doi.org/10.56499/jppres22.1392_10.5.800
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