J. Pharm. Pharmacogn. Res., vol. 10, no. 3, pp. 387-396, May-June 2022.
DOI: https://doi.org/10.56499/jppres21.1303_10.3.387
Original Article
Estudio farmacognóstico y capacidad antioxidante de la especie Smilax purhampuy Ruiz que crece en Ecuador
[Pharmacognostic study and antioxidant capacity of the Smilax purhampuy Ruiz species that grows in Ecuador]
Pilar A. Soledispa Cañarte1*, Raisa Mangas Marín2, Viviana García Mir3, Migdalia Miranda Martínez4†, Carolina F. Matute Jimbo1, Arlu I. Tello Mayorga1
1Departamento de Farmacia. Facultad de Ciencias Químicas. Universidad de Guayaquil. Ciudadela Universitaria “Salvador Allende”. Ave. Kennedy S/N y Av. Delta. Guayaquil, Ecuador.
2Departamento de Farmacia, Instituto de Farmacia y Alimentos, Universidad de La Habana, La Coronela, La Lisa, Habana 13600, Cuba.
3Universidad Técnica de Machala. Av. Panamericana Km 5 1/2 Vía a Pasaje. Machala. Ecuador.
4Departamento de Ciencias Químicas y Ambientales. Facultad de Ciencias Naturales y Matemáticas. Escuela Superior Politécnica del Litoral, ESPOL. Campus Gustavo Galindo Km. 30.5 Vía Perimetral, P.O. Box 09-01-5863. Guayaquil, Ecuador.
*E-mail: pilar.soledispac@ug.edu.ec
Abstract
Context: Smilax purhampuy Ruiz (sarsaparilla) is traditionally used to treat various ailments such as sudorific, diuretic, purifying, hypoglycemic, arthritis, dermatological infections, intestinal, stomach and prostate inflammations, vaginitis and viral diseases. However, the investigations that justify the use of this species are scarce.
Aims: To evaluate pharmacognostically and chemically leaves and rhizomes of sarsaparilla, and their antioxidant capacity.
Methods: The pharmacognostic analysis of both plant organs was carried out from the determination of their physicochemical parameters, the phytochemical screening, the quantification of phenols and total flavonoids and the evaluation of the antioxidant capacity by three in vitro methods (FRAP, DPPH and ABTS).
Results: The leaves and rhizomes of the species showed a similar chemical composition, although some metabolites seem to be found at different concentrations. Although the two organs studied presented antioxidant activity, it was higher in the leaves, which could be associated with the higher content of phenols and flavonoids.
Conclusions: The results obtained made it possible to report the quality parameters for the first time and suggest the chemical composition of both organs, showing some differences between them. The evaluation of the antioxidant capacity demonstrated the potential of the species to be used as a natural remedy.
Keywords: antioxidant; leaves; phenolic compounds; rhizomes; sarsaparilla.
Resumen
Contexto: Smilax purhampuy Ruiz (zarzaparrilla) se usa tradicionalmente para tratar diversas dolencias como sudorífica, diurético, depurativa, hipoglucemiante, artritis, infecciones dermatológicas, inflamaciones intestinales, estomacales y de la próstata, vaginitis y enfermedades virales. Sin embargo, las investigaciones que justifiquen el empleo de esta especie son escasas.
Objetivos: Evaluar farmacognóstica y químicamente las hojas y rizomas de zarzaparrilla y su capacidad antioxidante.
Métodos: Se realizó el análisis farmacognóstico de ambos órganos vegetales a partir de la determinación de sus parámetros fisicoquímicos, el tamizaje fitoquímico, la cuantificación de fenoles y flavonoides totales y la evaluación de la capacidad antioxidante por tres métodos in vitro (FRAP, DPPH y ABTS).
Resultados: Las hojas y rizomas de la especie mostraron una composición química similar, aunque algunos metabolitos parecen encontrarse a diferentes concentraciones. Aunque los dos órganos estudiados presentaron actividad antioxidante, fue superior en las hojas, lo que pudiera asociarse al mayor contenido de fenoles y flavonoides.
Conclusiones: Los resultados obtenidos permitieron informar por primera vez los parámetros de calidad y sugerir la composición química de ambos órganos evidenciándose algunas diferencias entre ellos. La evaluación de la capacidad antioxidante demostró las potencialidades de la especie para ser utilizada como remedio natural.
Palabras Clave: diabetes mellitus; hiperglucemantioxidante; compuestos fenólicos; hojas; rizomas; zarzaparrilla.
Citation Format: Soledispa PA, Mangas R, García V, Miranda M, Matute CF, Tello AI (2022) Estudio farmacognóstico y capacidad antioxidante de la especie Smilax purhampuy Ruiz que crece en Ecuador. [Pharmacognostic study and antioxidant capacity of the Smilax purhampuy Ruiz species that grows in Ecuador]. J Pharm Pharmacogn Res 10(3): 387–396. https://doi.org/10.56499/jppres21.1303_10.3.387
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