Tag Archives: oxidative status

Antidiabetic potential of Ecobolium linneanum leaf extracts

J. Pharm. Pharmacogn. Res., vol. 10, no. 3, pp. 496-507, May-June 2022.

Original Article

Antidiabetic potential of leaf extracts of Ecobolium linneanum Kurz in streptozotocin-induced diabetic rats

[Potencial antidiabético de extractos de hojas de Ecobolium linneanum Kurz en ratas diabéticas inducidas por estreptozotocina]

Srilakshmi Nallapaty1, Narender Malothu1*, Rajeswari Pasupula2,Alekhya Ketha3

1Department of Pharmaceutical Chemistry, KL College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, AP, 522 502, India.

2Department of Pharmacology, KL College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, AP, 522 502, India.

3Department of Pharmaceutical Chemistry, AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, AP, India.

*E-mail: mnarender@kluniversity.in, narendermalothu@gmail.com


Context: In a general context, the importance’s of several medicinal plants has been explored in treating diabetes mellitus. In this connection, the antidiabetic potentiality needs to be established for one of the medicinally emerging plants Ecbolium linneanum Kurz.

Aims: To evaluate the antidiabetic activity of petroleum ether, ethyl acetate, and methanol extracts of E. linneanum leaves in streptozotocin-induced diabetic rats.

Methods: Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg BW) in male Wistar rats. Diabetic rats were treated orally with petroleum ether, ethyl acetate and methanol extracts of E. linneanum leaves at two doses (100 and 200 mg/kg BW/day) for 14 days. The blood glucose, lipid profile, serum enzyme levels, glycosylated haemoglobin (HbA1c), creatinine and serum urea levels were monitored. Glibenclamide (10 mg/kg BW) was used as a standard hypoglycaemic drug.

Results: The methanolic leaf extract at 200 mg/kg BW showed increasingly substantial (p<0.001) antidiabetic activity on day 14 and also significantly reduced (p<0.001) SGOT, SGPT, TG, TC, VLDL, LDL, HbA1c and antioxidant enzymes (SOD, CAT and GSH levels) increased significantly (p<0.001) compared to diabetic group. Renal parameters also decreased significantly (p<0.001) at both doses relative to diabetic control, but petroleum leaf extract and ethyl acetate leaf extract exhibited a less significant effect (p<0.05) compared to the methanol extract. All the extracts of leaves showed a protective effect on the oxidative status of the liver at tested concentrations (100 and 200 mg/kg BW).

Conclusions: The present research findings revealed that E. linneanum leaf possesses significant anti-hyperglycaemic activity and encourages conventional use in the treatment of diabetes mellitus under the experimental conditions exposed in this study.

Keywords: antidiabetic activity; Ecbolium linneanum; oxidative status; phytochemical screening; streptozotocin.

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Contexto: Se ha explorado la importancia de varias plantas medicinales en el tratamiento de la diabetes mellitus. A este respecto, es necesario establecer la potencialidad antidiabética de una de las plantas medicinales emergentes Ecbolium linneanum Kurz.

Objetivos: Evaluar la actividad antidiabética de extractos de éter de petróleo, acetato de etilo y metanol de hojas de E. linneanum en ratas diabéticas inducidas por estreptozotocina.

Métodos: Se indujo diabetes mediante inyección intraperitoneal de estreptozotocina (60 mg/kg de peso corporal) en ratas Wistar macho. Ratas diabéticas fueron tratadas por vía oral con extractos de éter de petróleo, acetato de etilo y metanol de hojas de E. linneanum en dos dosis (100 y 200 mg/kg PC/día) durante 14 días. Se monitorizaron los niveles de glucosa en sangre, perfil lipídico, enzimas séricas, hemoglobina glicosilada (HbA1c), creatinina y urea sérica. Se utilizó glibenclamida (10 mg/kg de peso corporal) como fármaco hipoglucemiante estándar.

Resultados: El extracto de hoja metanólico a 200 mg/kg de peso corporal mostró una actividad antidiabética cada vez más sustancial (p<0,001) en el día 14 y también redujo significativamente (p<0,001) SGOT, SGPT, TG, TC, VLDL, LDL, HbA1c y enzimas antioxidantes (niveles de SOD, CAT y GSH) aumentaron significativamente (p<0,001) en comparación con el grupo de diabéticos. Los parámetros renales también disminuyeron significativamente (p<0,001) en ambas dosis en relación con el control diabético, pero el extracto de hoja de petróleo y el extracto de hoja de acetato de etilo exhibieron un efecto menos significativo (p<0,05) en comparación con el extracto metanólico. Todos los extractos de hojas mostraron un efecto protector sobre el estado oxidativo del hígado a las concentraciones probadas (100 y 200 mg/kg de peso corporal).

Conclusiones: Los hallazgos de la presente investigación revelaron que la hoja de E. linneanum posee una importante actividad antihiperglucémica y fomenta el uso convencional en el tratamiento de la diabetes mellitus en las condiciones experimentales expuestas en este estudio.

Palabras Clave: actividad antidiabética; Ecbolium linneanum; estado oxidativo; estreptozotocina; tamizaje fitoquímico.

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Citation Format: Srilakshmi N, Narender M, Rajeswari P, Alekhya K (2022) Antidiabetic potential of leaf extracts of Ecobolium linneanum Kurz in streptozotocin-induced diabetic rats. J Pharm Pharmacogn Res 10(3): 496–507.

Adeneya AA, Olagunju J (2009) Preliminary hypoglycemic and hypolipidemic activities of the aqueous seed extract of Carica papaya Linn. in Wistar rats. Biol Med 1: 1-10.

Arkkelin D (2014) Using SPSS to Understand Research and Data Analysis. Psy Curr Mat 1: 1-195.

Baquer NZ, Kumar P, Taha A, Kale R, Cowsik S, McLean P (2011) Metabolic and molecular action of Trigonella foenum-graecum (fenugreek) and trace metals in experimental diabetic tissues. J Biosci 36(2): 383–396.

Bartoli E, Fra GP, Schianca GC (2011) The oral glucose tolerance test (OGTT) revisited. Eur J Intern Med 22(1): 8-12.

Chen X, Fang M (2018) Oxidative stress mediated mitochondrial damage plays roles in pathogenesis of diabetic nephropathy rat. Eur Rev Med Pharmacol Sci 22 (16): 5248-5254.

Cressey R, Kumsaiyai W, Mangklabruks A (2014) Daily consumption of banana marginally improves blood glucose and lipid profile in hypercholesterolemic subjects and increases serum adiponectin in type 2 diabetic patients. Indian J Exp Biol 52: 1173-1181.

Czeczot H, Scibior D, Skrzycki M, Podsiad M (2006) Activity of antioxidant enzymes in patients with liver cirrhosis.Wiadi Lek 59(11-12): 762-766.

Diyya K, Priya GV, Rao BG (2014) Pharmacognosy of Ecbolium viride. A review. Int J Pharmacogn Phytochem 6(1): 45-52.

Diyya K, Rao B (2018) Stability indicating method development and validation for the simultaneous estimation of orientin, vitexin and luteolin in Ecbolium viride (Forssk) Alston. Int J Pharm Res 10(2): 27-33.

Dumala N, Mangalampalli B, Kamal SSK, Grover P (2018) Biochemical alterations induced by nickel oxide nanoparticles in female Wistar albino rats after acute oral exposure. J Biomark 23: 33-43

Ekrem S, Mustafa A, Erdem Y, Shigeru I (2005) Hypoglycaemic activity of Gentiana olivieri and isolation of the active constituent through bioassay-directed fractionation techniques. Life Sci 76: 1223–1238.

Folin O, Wu H (1920) A simplified and improved method for determination of sugar. J Biol Chem 41: 367–374.

Ghani A (1998) Medicinal plants of Bangladesh: chemical constituents and uses. 2nd ed. Asiatic Society of Bangladesh.

Ghosh T, Kumar MT, Sengupta P, Dash DK, Bose A (2008) Antidiabetic and in vivo antioxidant activity of ethanolic extract of Bacopa monnieri Linn. aerial parts: a possible mechanism of action. Iran J Pharm Res 7: 61-68.

Lenzen S (2008) The mechanisms of alloxan-and streptozotocin-induced diabetes. Diabetologia 51(2): 216-226.

Lopa AF, Jannat K, Hamid A, Rahmatullah M (2018) Oral glucose tolerance tests with methanolic extract of fruits of Musa textilis Nee. J Med Plants Stud 6(5): 130-132.

Mojani MS, Sarmadi VH, Vellasamy S, Sandrasaigaran P, Rahmat A, Peng LS, Ramasamy R (2014) Evaluation of metabolic and immunological changes in streptozotocin-nicotinamide induced diabetic rats. Cell Immunol 289(1-2): 145-149.

Morsy MD, Hassan WN, Zalat SI (2010) Improvement of renal oxidative stress markers after ozone administration in diabetic nephropathy in rats. Diabetol Metab Syndr 2(1): 29.

Murea M, Ma L, Freedman BI (2012) Genetic and environmental factors associated with type 2 diabetes and diabetic vascular complications. Rev Diabet Stud 9(1): 6.

Neelutpal G, Biman B (2017) In vivo antidiabetic activity evaluation of the bark of Cascabela thevetia L. in streptozotocin induced diabetic rats. Int J Pharm Pharm Sci 9: 48-53.

OECD (2002) Test No. 420: Acute Oral Toxicity – Fixed Dose Procedure. OECD Guidelines for the Testing of Chemicals, Section 4, Paris: OECD Publishing.

Rao GM, Morghom LO, Kabur MN, Ben Mohmad BM, Ashiboni K (1989) Serum glutamic oxaloacetic transaminase (GOT) and glutamic pyruvate transaminase (GPT) levels in diabetes mellitus. Indian J Med Sci 43: 118-122.

Rathor RB, Rao DR, Rao P (2013) Phytochemical sreening and antidiabetic antioxidant effect of Ecbolium ligustrinum flowers extracts. Indian J Res Pharm Biotechnol 1(4): 575-580.

Sachan NK, Kumar Y, Pushkar S, Thakur RN, Gangwar SS (2009) Antidiabetic potential of alcoholic and aqueous extracts of Ficus. Int J Pharm Sci Res 1: 24-27.

Sharifi-Rad M, Nazaruk J, Polito L, Morais-Braga MFB, Rocha JE, Coutinho HDM, Salehi B, Tabanelli G, Montanari C, del Mar Contreras M, Yousaf Z (2018) Matricaria genus as a source of antimicrobial agents: from farm to pharmacy and food applications. Microbiol Res 215: 76-88.

Sharma R, Sharma HK (2010) Ethnomedicine of Sonarpur, Kamrup district, Assam. Ind J Trad Know 9: 163-165.

Sherwani SI, Khan HA, Ekhzaimy A, Masood A, Sakharkar MK (2016) Significance of HbA1c test in diagnosis and prognosis of diabetic patients. Biomark Insights 11: 95-104.

Srilakshmi N, Umasankar K, Rao GSNK, Panda SP, Reddy AR (2019) Antidiabetic activity of Chrozophora rottleri leaves extracts in streptozotocin-induced diabetic rats. Int J Pharm Res 11(4): 978-988.

The Wealth of India (2006) Raw Material. New Delhi, CSIR 3: 123.

Trease GE, Evans WC (1989) Introduction and General Methods in Pharmacognosy. 13th Eds, United Kingdom: Cambridge University Press.

Viet Hieu HO, Tatipamula VB, Killari KN, Koneru ST, Srilakshmi N, Ranajith SK (2020) HPTLC analysis, antioxidant and antidiabetic activities of ethanol extract of moss Fissidens grandiflora. Indian J Pharm Sci 8(3): 449-455.

Zhong Y, Fan H, Zhang R, Wang H, Zhang W, Tian T (2019) Associations between HbA1c variability index and risk of chronic complications of diabetes mellitus. Chin Gen Pra 23(3): 276-288.

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