Tag Archives: phenolic compounds

Antioxidant and antimicrobial activity of Senecio nutans

J. Pharm. Pharmacogn. Res., vol. 10, no. 6, pp. 1026-1036, November-December 2022.

DOI: https://doi.org/10.56499/jppres22.1471_10.6.1026

Original Article

In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae)

[Propiedades antioxidantes in vitro y actividad antimicrobiana del extracto etanólico de Senecio nutans Sch. Beep. (Asteraceae)]

Felipe Surco-Laos1, Jorge A. Garcia1, María R. Bendezú1, Doris Laos-Anchante1, Juan F. Panay-Centeno1, Manuel Valle-Campos1, Juan J. Palomino-Jhong1, Paulina Eliades Yarasca-Carlos2, Berta Loja3, Angel T. Alvarado4*

1Faculty of Pharmacy and Biochemistry, San Luis Gonzaga National University of Ica, 11004, Ica, Peru.

2Biological Sciences Faculty, San Luis Gonzaga National University of Ica, 11004, Ica, Peru.

3Environmental Engineering, San Ignacio de Loyola University, La Molina 15024, Lima, Peru.

4International Research Network of Pharmacology and Precision Medicine (REDIFMEP), San Ignacio de Loyola University, La Molina 15024, Lima, Peru.

*E-mail: angel.alvarado@usil.pe

Abstract

Context: Senecio nutans Sch. Beep. is used by the Andean population of Ayacucho-Peru as traditional medicine for various health problems.

Aims: To evaluate the antioxidant and antimicrobial activity of the ethanolic extract of leaves of S. nutans against the enteropathogen Escherichia coli and the filamentous fungus Aspergillus niger.

Methods: The antioxidant capacity of the ethanolic extract was evaluated by ABTS, DPPH, and FRAP assays; the antimicrobial activity was evaluated by the agar diffusion method, and determining the minimum inhibitory concentration (MIC), minimum bactericidal (MBC), and fungicidal concentration (MFC).

Results: Polyphenols, terpenes, and tannins were identified; the total polyphenolic content was 67.91 ± 0.29 mg GAE/g. The ethanolic extract at 20 mg/mL showed moderate antibacterial activity (79.14 ± 0.02% inhibition, MIC and MBC >400 μg/mL on E. coli), and antifungal (88.90 ± 0.17% inhibition, MIC and MCF >400 μg/mL on A. niger); radical scavenging capacity (ABTS 10.31 ± 0.09 mM TE/g; DPPH 8.28 ± 0.07 IC50 µg/mL) and iron reducing power (FRAP 17.72 ± 0.11 mM TE/g). S. nutans turned out to be a potential antimicrobial and antioxidant species associated with the presence of its bioactive components, for which further investigation is warranted.

Conclusions: The ethanolic extract of leaves S. nutans showed moderate activity against pathogenic bacteria (E. coli) and filamentous fungus (A. niger), as well as antioxidant activity in three in vitro methods.

Keywords: antimicrobial activity; chachacoma; in vitro antioxidant activity; phenolic compounds; Senecio nutans.

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Resumen

Contexto: Senecio nutans Sch. Beep. es utilizada por la población andina de Ayacucho-Perú como medicina tradicional en diversos problemas de salud.

Objetivos: Evaluar la actividad antioxidante y antimicrobiana del extracto etanólico de hojas de S. nutans frente al enteropatógeno Escherichia coli y el hongo filamentoso Aspergillus niger.

Métodos: La capacidad antioxidante del extracto etanólico fue evaluada mediante los ensayos de ABTS, DPPH y FRAP; la actividad antimicrobiana se evaluó mediante el método de difusión en agar, y determinando la concentración inhibitoria mínima (MIC), concentración bactericida (MBC) y fungicida mínimo (MCF).

Resultados: Se identificó polifenoles, terpenos y taninos; el contenido polifenólico total fue de 67,91 ± 0,29 mg GAE/g. El extracto etanólico a 20 mg/mL mostró actividad antibacteriana moderada (79,14 ± 0,02% de inhibición, MIC y MBC >400 μg/mL sobre E. coli), y antifúngica (88,90 ± 0,17 % de inhibición, MIC y MCF >400 μg/mL sobre A. niger); capacidad secuestradora de radicales (ABTS 10,31 ± 0,09 mM TE/g; DPPH 8,28 ± 0,07 IC50 µg/mL) y poder reductor del hierro (FRAP 17,72 ± 0,11 mM TE/g). S. nutans resultó ser una especie potencial como antimicrobiana y antioxidante, asociado a la presencia de sus componentes bioactivos, por lo que amerita mayor investigación.

Conclusiones: El extracto etanólico de las hojas de S. nutans mostró actividad moderada frente a la bacteria patógena (E. coli) y hongo filamentoso (A. niger), a la vez actividad antioxidante en tres métodos in vitro.

Palabras Clave: actividad antimicrobiana; actividad antioxidante in vitro; chachacoma; compuestos fenólicos; Senecio nutans.

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Citation Format: Surco F, García JA, Bendezú MR, Laos D, Panay JF, Valle M, Palomino JJ, Yarasca PE, Loja B, Alvarado AT (2022) In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae). J Pharm Pharmacogn Res 10(6): 1026–1036. https://doi.org/10.56499/jppres22.1471_10.6.1026
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© 2022 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

Evaluación farmacognóstica y antioxidante de zarzaparrilla

J. Pharm. Pharmacogn. Res., vol. 10, no. 3, pp. 387-396, May-June 2022.

DOI: https://doi.org/10.56499/jppres21.1303_10.3.387

Original Article

Estudio farmacognóstico y capacidad antioxidante de la especie Smilax purhampuy Ruiz que crece en Ecuador

[Pharmacognostic study and antioxidant capacity of the Smilax purhampuy Ruiz species that grows in Ecuador]

Pilar A. Soledispa Cañarte1*, Raisa Mangas Marín2, Viviana García Mir3, Migdalia Miranda Martínez4†, Carolina F. Matute Jimbo1, Arlu I. Tello Mayorga1

1Departamento de Farmacia. Facultad de Ciencias Químicas. Universidad de Guayaquil. Ciudadela Universitaria “Salvador Allende”. Ave. Kennedy S/N y Av. Delta. Guayaquil, Ecuador.

2Departamento de Farmacia, Instituto de Farmacia y Alimentos, Universidad de La Habana, La Coronela, La Lisa, Habana 13600, Cuba.

3Universidad Técnica de Machala. Av. Panamericana Km 5 1/2 Vía a Pasaje. Machala. Ecuador.

4Departamento de Ciencias Químicas y Ambientales. Facultad de Ciencias Naturales y Matemáticas. Escuela Superior Politécnica del Litoral, ESPOL. Campus Gustavo Galindo Km. 30.5 Vía Perimetral, P.O. Box 09-01-5863. Guayaquil, Ecuador.

*E-mail: pilar.soledispac@ug.edu.ec

Abstract

Context: Smilax purhampuy Ruiz (sarsaparilla) is traditionally used to treat various ailments such as sudorific, diuretic, purifying, hypoglycemic, arthritis, dermatological infections, intestinal, stomach and prostate inflammations, vaginitis and viral diseases. However, the investigations that justify the use of this species are scarce.

Aims: To evaluate pharmacognostically and chemically leaves and rhizomes of sarsaparilla, and their antioxidant capacity.

Methods: The pharmacognostic analysis of both plant organs was carried out from the determination of their physicochemical parameters, the phytochemical screening, the quantification of phenols and total flavonoids and the evaluation of the antioxidant capacity by three in vitro methods (FRAP, DPPH and ABTS).

Results: The leaves and rhizomes of the species showed a similar chemical composition, although some metabolites seem to be found at different concentrations. Although the two organs studied presented antioxidant activity, it was higher in the leaves, which could be associated with the higher content of phenols and flavonoids.

Conclusions: The results obtained made it possible to report the quality parameters for the first time and suggest the chemical composition of both organs, showing some differences between them. The evaluation of the antioxidant capacity demonstrated the potential of the species to be used as a natural remedy.

Keywords: antioxidant; leaves; phenolic compounds; rhizomes; sarsaparilla.

Resumen

Contexto: Smilax purhampuy Ruiz (zarzaparrilla) se usa tradicionalmente para tratar diversas dolencias como sudorífica, diurético, depurativa, hipoglucemiante, artritis, infecciones dermatológicas, inflamaciones intestinales, estomacales y de la próstata, vaginitis y enfermedades virales. Sin embargo, las investigaciones que justifiquen el empleo de esta especie son escasas.

Objetivos: Evaluar farmacognóstica y químicamente las hojas y rizomas de zarzaparrilla y su capacidad antioxidante.

Métodos: Se realizó el análisis farmacognóstico de ambos órganos vegetales a partir de la determinación de sus parámetros fisicoquímicos, el tamizaje fitoquímico, la cuantificación de fenoles y flavonoides totales y la evaluación de la capacidad antioxidante por tres métodos in vitro (FRAP, DPPH y ABTS).

Resultados: Las hojas y rizomas de la especie mostraron una composición química similar, aunque algunos metabolitos parecen encontrarse a diferentes concentraciones. Aunque los dos órganos estudiados presentaron actividad antioxidante, fue superior en las hojas, lo que pudiera asociarse al mayor contenido de fenoles y flavonoides.

Conclusiones: Los resultados obtenidos permitieron informar por primera vez los parámetros de calidad y sugerir la composición química de ambos órganos evidenciándose algunas diferencias entre ellos. La evaluación de la capacidad antioxidante demostró las potencialidades de la especie para ser utilizada como remedio natural.

Palabras Clave: diabetes mellitus; hiperglucemantioxidante; compuestos fenólicos; hojas; rizomas; zarzaparrilla.

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Citation Format: Soledispa PA, Mangas R, García V, Miranda M, Matute CF, Tello AI (2022) Estudio farmacognóstico y capacidad antioxidante de la especie Smilax purhampuy Ruiz que crece en Ecuador. [Pharmacognostic study and antioxidant capacity of the Smilax purhampuy Ruiz species that grows in Ecuador]. J Pharm Pharmacogn Res 10(3): 387–396. https://doi.org/10.56499/jppres21.1303_10.3.387
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