Tag Archives: streptozotocin

Antidiabetic potential of Ecobolium linneanum leaf extracts

J. Pharm. Pharmacogn. Res., vol. 10, no. 3, pp. 496-507, May-June 2022.

Original Article

Antidiabetic potential of leaf extracts of Ecobolium linneanum Kurz in streptozotocin-induced diabetic rats

[Potencial antidiabético de extractos de hojas de Ecobolium linneanum Kurz en ratas diabéticas inducidas por estreptozotocina]

Srilakshmi Nallapaty1, Narender Malothu1*, Rajeswari Pasupula2,Alekhya Ketha3

1Department of Pharmaceutical Chemistry, KL College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, AP, 522 502, India.

2Department of Pharmacology, KL College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, AP, 522 502, India.

3Department of Pharmaceutical Chemistry, AU College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, AP, India.

*E-mail: mnarender@kluniversity.in, narendermalothu@gmail.com

Abstract

Context: In a general context, the importance’s of several medicinal plants has been explored in treating diabetes mellitus. In this connection, the antidiabetic potentiality needs to be established for one of the medicinally emerging plants Ecbolium linneanum Kurz.

Aims: To evaluate the antidiabetic activity of petroleum ether, ethyl acetate, and methanol extracts of E. linneanum leaves in streptozotocin-induced diabetic rats.

Methods: Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg BW) in male Wistar rats. Diabetic rats were treated orally with petroleum ether, ethyl acetate and methanol extracts of E. linneanum leaves at two doses (100 and 200 mg/kg BW/day) for 14 days. The blood glucose, lipid profile, serum enzyme levels, glycosylated haemoglobin (HbA1c), creatinine and serum urea levels were monitored. Glibenclamide (10 mg/kg BW) was used as a standard hypoglycaemic drug.

Results: The methanolic leaf extract at 200 mg/kg BW showed increasingly substantial (p<0.001) antidiabetic activity on day 14 and also significantly reduced (p<0.001) SGOT, SGPT, TG, TC, VLDL, LDL, HbA1c and antioxidant enzymes (SOD, CAT and GSH levels) increased significantly (p<0.001) compared to diabetic group. Renal parameters also decreased significantly (p<0.001) at both doses relative to diabetic control, but petroleum leaf extract and ethyl acetate leaf extract exhibited a less significant effect (p<0.05) compared to the methanol extract. All the extracts of leaves showed a protective effect on the oxidative status of the liver at tested concentrations (100 and 200 mg/kg BW).

Conclusions: The present research findings revealed that E. linneanum leaf possesses significant anti-hyperglycaemic activity and encourages conventional use in the treatment of diabetes mellitus under the experimental conditions exposed in this study.

Keywords: antidiabetic activity; Ecbolium linneanum; oxidative status; phytochemical screening; streptozotocin.

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Resumen

Contexto: Se ha explorado la importancia de varias plantas medicinales en el tratamiento de la diabetes mellitus. A este respecto, es necesario establecer la potencialidad antidiabética de una de las plantas medicinales emergentes Ecbolium linneanum Kurz.

Objetivos: Evaluar la actividad antidiabética de extractos de éter de petróleo, acetato de etilo y metanol de hojas de E. linneanum en ratas diabéticas inducidas por estreptozotocina.

Métodos: Se indujo diabetes mediante inyección intraperitoneal de estreptozotocina (60 mg/kg de peso corporal) en ratas Wistar macho. Ratas diabéticas fueron tratadas por vía oral con extractos de éter de petróleo, acetato de etilo y metanol de hojas de E. linneanum en dos dosis (100 y 200 mg/kg PC/día) durante 14 días. Se monitorizaron los niveles de glucosa en sangre, perfil lipídico, enzimas séricas, hemoglobina glicosilada (HbA1c), creatinina y urea sérica. Se utilizó glibenclamida (10 mg/kg de peso corporal) como fármaco hipoglucemiante estándar.

Resultados: El extracto de hoja metanólico a 200 mg/kg de peso corporal mostró una actividad antidiabética cada vez más sustancial (p<0,001) en el día 14 y también redujo significativamente (p<0,001) SGOT, SGPT, TG, TC, VLDL, LDL, HbA1c y enzimas antioxidantes (niveles de SOD, CAT y GSH) aumentaron significativamente (p<0,001) en comparación con el grupo de diabéticos. Los parámetros renales también disminuyeron significativamente (p<0,001) en ambas dosis en relación con el control diabético, pero el extracto de hoja de petróleo y el extracto de hoja de acetato de etilo exhibieron un efecto menos significativo (p<0,05) en comparación con el extracto metanólico. Todos los extractos de hojas mostraron un efecto protector sobre el estado oxidativo del hígado a las concentraciones probadas (100 y 200 mg/kg de peso corporal).

Conclusiones: Los hallazgos de la presente investigación revelaron que la hoja de E. linneanum posee una importante actividad antihiperglucémica y fomenta el uso convencional en el tratamiento de la diabetes mellitus en las condiciones experimentales expuestas en este estudio.

Palabras Clave: actividad antidiabética; Ecbolium linneanum; estado oxidativo; estreptozotocina; tamizaje fitoquímico.

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Citation Format: Srilakshmi N, Narender M, Rajeswari P, Alekhya K (2022) Antidiabetic potential of leaf extracts of Ecobolium linneanum Kurz in streptozotocin-induced diabetic rats. J Pharm Pharmacogn Res 10(3): 496–507.
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© 2022 Journal of Pharmacy & Pharmacognosy Research (JPPRes)

Hibiscus surattensis: Insulin secretion and pancreatic tissue improvement



J Pharm Pharmacogn Res 9(4): 454-464, 2021.

Original article

Insulin secretion and repairing pancreatic tissue damage on diabetic mice treated with the extract and active fraction of Hibiscus surattensis L. leaves

[Secreción de insulina y reparación del daño del tejido pancreático en ratones diabéticos tratados con el extracto y la fracción activa de hojas de Hibiscus surattensis L.]

Yuliet1*, Elin Yulinah Sukandar2, Nur Atik3, I Ketut Adnyana2

1Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University, Palu 94148, Central Sulawesi, Indonesia.

2Department of Pharmacology and Toxicology, School of Pharmacy, Bandung Institute of Technology, Bandung 40132, West Java, Indonesia.

3Department of Biomedical Sciences, Faculty of Medicine, Padjadjaran University, Jatinangor 45363, West Java, Indonesia.

*E-mail: yuliet@untad.ac.id; yuliet_susanto@yahoo.com

Abstract

Context: Diabetes is a chronic health condition with insulin disturbance. Hibiscus surattensis L. is a medicinal plant and plays a pivotal role in diabetes.

Aims: To evaluate the effect of H. surattensis leaves extract to increase insulin secretion and repair pancreatic tissue damage in streptozotocin (STZ)‑induced diabetic mice.

Methods: This research used male mice (Swiss Webster) administrated by STZ 100 mg/kg BW to induce diabetes. Animals were divided into eight groups (n = 6) consisting of normal control, diabetic control, glibenclamide, ethanol extract 50, ethyl acetate fraction, and water fraction with doses of 25 and 50 mg/kg BW, respectively. Treatment was given for 28 days. The evaluation was carried out on blood glucose levels, insulin levels, HOMA-β, and the pancreatic islets by the morphometric analysis were evaluated. All data were analyzed using SPSS 16.0.

Results: Extract and active fraction of H. surattensis leaves, especially ethyl acetate fractions (25 and 50 mg/kg BW) significantly (p<0.05) reduced fasting blood glucose levels, insulin levels, and the strength of pancreatic β cells (HOMA-β) increased in STZ induced diabetic mice. Histopathological results in pancreatic confirmed the effect of H. surattensis to increase the number of islets significantly in Langerhans and pancreatic β-cells compared to the diabetic control group (p<0.05).

Conclusions: Extract, and active fraction of H. surattensis leaves affect the insulin secretion and repairing the pancreatic tissue damage on diabetic mice, the most optimal therapy on the administration of ethyl acetate fraction.

Keywords: antidiabetic; Hibiscus surattensis L.; insulin; pancreas; streptozotocin.

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Resumen

Contexto: La diabetes es una enfermedad crónica con alteración de la insulina. Hibiscus surattensis L. es una planta medicinal y juega un papel fundamental en la diabetes.

Objetivos: Evaluar el efecto del extracto de hojas de H. surattensis para aumentar la secreción de insulina y reparar el daño del tejido pancreático en ratones diabéticos inducidos por estreptozotocina (STZ).

Métodos: Esta investigación utilizó ratones macho (Swiss Webster) administrados por STZ 100 mg/kg BW para inducir diabetes. Los animales se dividieron en ocho grupos (n = 6) que consistían en control normal, control diabético, glibenclamida, extracto de etanol 50, fracción de acetato de etilo y fracción de agua con dosis de 25 y 50 mg/kg de peso corporal, respectivamente. El tratamiento se administró durante 28 días. La evaluación se realizó sobre niveles de glucosa en sangre, niveles de insulina, HOMA-β, y se evaluaron los islotes pancreáticos mediante análisis morfométrico. Todos los datos se analizaron con SPSS 16.0.

Resultados: El extracto y la fracción activa de hojas de H. surattensis, especialmente las fracciones de acetato de etilo (25 y 50 mg/kg de peso corporal) redujeron significativamente (p<0,05) los niveles de glucosa en sangre en ayunas, los niveles de insulina y la fuerza de las células β pancreáticas (HOMA-β) aumentó en ratones diabéticos inducidos por STZ. Los resultados histopatológicos en páncreas confirmaron el efecto de H. surattensis para aumentar significativamente el número de islotes en Langerhans y células β pancreáticas en comparación con el grupo de control diabético (p<0,05).

Conclusiones: El extracto y fracción activa de hojas de H. surattensis afectan la secreción de insulina y reparan el daño del tejido pancreático en ratones diabéticos, la terapia más óptima sobre la administración de fracción de acetato de etilo.

Palabras Clave: antidiabético; estreptozotocina; Hibiscus surattensis L.; insulina; páncreas.

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Citation Format: Yuliet, Sukandar EY, Atik N, Adnyana IK (2021) Insulin secretion and repairing pancreatic tissue damage on diabetic mice treated with the extract and active fraction of Hibiscus surattensis L. leaves. J Pharm Pharmacogn Res 9(4): 454–464.

© 2021 Journal of Pharmacy & Pharmacognosy Research (JPPRes)